thank you for this video, very interesting! One thing seems odd to me: Shouldn't the SNARE Complex and the vesicle at 2:29 be located within the axon/at the presynaptic membrane?
@@quinattasneemrafique536 No, their drawing at 2:29 is confusingly wrong, and there IS no such thing as a 'post synaptic CLEFT'. There is a PRE-synaptic NEURON (the important part of which is the axon terminal containing the vesicles), the synaptic CLEFT (the gap between the two neurons), and the POST-synaptic NEURON (the important part of which would be a LIGAND-GATED RECEPTOR PORE(not shown, which the diffused neurotransmitters bind to and cause to open). I'd say that what's wrong with the drawing there is the presence of the GREEN 'object' in the drawing, because it LOOKS like an Axon Terminal, within which the fusion should be shown to occur, and that green object's presence makes their drawing confusing because it seems to show the fusing action taking place OUTSIDE the axon terminal (= bouton). It seems to me that what they are TRYING to show, (if you ignore and remove the green shape), is that the YELLOW line is supposed to be the target membrane of the pre-synaptic neuron, and the CREME colored area below it is actually the synaptic CLEFT itself, into which the neurotransmitters should be expressed upon fusion of the vesicle to the pre-synaptic neuron's membrane. And then the drawing's words at the bottom left should NOT be 'target membrane', but rather SYNAPTIC CLEFT. If you make those changes to the drawing (or if you were to see the actions with the SNARE proteins happening WITHIN the green object, as if it were in fact the axon terminal that it looks like), then what they've shown at 2: 29 would be correct and useful.
Best illustration about the cellular process...Your videos helps students for easy imagination while reading....Thank you for this wonderful job...We need more of it 😀😀All the best for your growth 😄
Hello, thanks for the clip, very graphic and explanatory. I have a question though, you said something which confused me. In minute 2:42, you say "SNAP-25, forms coils with another t-SNARE called synaptobrevin, and the v-SNARE syntaxin". Isnt the other way around? t-SNARE called syntaxin and v-SNARE called synaptobrevin? Im sorry for the detail, but Im having a hard time figuring this process out. Thanks
@@CellClips thank you so much for the clarification! This subjects are so complex to me, that trying to follow is really hard, so I gotta go step by step. And that kind of took me by surprise! But your video reaaly helped me a lot in understanding this better. Bests!
Very informative and well-produced video, thank you! One question: if the Rab's are specific for different membranes, how is that specificity granted? Are the GEFs on the membranes selective for a corresponding Rab? If so, how does a target membrane get rid of the now incorporated Rab-GEFs after fusing with a vesicle that contained them to recruit their corresponding Rab? Thank you in advance for any answers!
these videos really help me considering that my class does not have lectures at all and only requires us to read -.-
thank you for this video, very interesting! One thing seems odd to me: Shouldn't the SNARE Complex and the vesicle at 2:29 be located within the axon/at the presynaptic membrane?
indeed, they located the vesicle outside the presynaptic nerve terminal... this is unfortunately not usable for teaching
oops.
I believe there is nothing wrong... The SNARE complex is in the post synaptic cleft.
@@quinattasneemrafique536 No, their drawing at 2:29 is confusingly wrong, and there IS no such thing as a 'post synaptic CLEFT'. There is a PRE-synaptic NEURON (the important part of which is the axon terminal containing the vesicles), the synaptic CLEFT (the gap between the two neurons), and the POST-synaptic NEURON (the important part of which would be a LIGAND-GATED RECEPTOR PORE(not shown, which the diffused neurotransmitters bind to and cause to open).
I'd say that what's wrong with the drawing there is the presence of the GREEN 'object' in the drawing, because it LOOKS like an Axon Terminal, within which the fusion should be shown to occur, and that green object's presence makes their drawing confusing because it seems to show the fusing action taking place OUTSIDE the axon terminal (= bouton).
It seems to me that what they are TRYING to show, (if you ignore and remove the green shape), is that the YELLOW line is supposed to be the target membrane of the pre-synaptic neuron, and the CREME colored area below it is actually the synaptic CLEFT itself, into which the neurotransmitters should be expressed upon fusion of the vesicle to the pre-synaptic neuron's membrane.
And then the drawing's words at the bottom left should NOT be 'target membrane', but rather SYNAPTIC CLEFT.
If you make those changes to the drawing (or if you were to see the actions with the SNARE proteins happening WITHIN the green object, as if it were in fact the axon terminal that it looks like), then what they've shown at 2: 29 would be correct and useful.
Best illustration about the cellular process...Your videos helps students for easy imagination while reading....Thank you for this wonderful job...We need more of it 😀😀All the best for your growth 😄
wow, excellent video. thank you for the clear explanation and accompanying illustrations!
best videos ever
Hello, thanks for the clip, very graphic and explanatory. I have a question though, you said something which confused me. In minute 2:42, you say "SNAP-25, forms coils with another t-SNARE called synaptobrevin, and the v-SNARE syntaxin". Isnt the other way around? t-SNARE called syntaxin and v-SNARE called synaptobrevin? Im sorry for the detail, but Im having a hard time figuring this process out. Thanks
That is correct, I must have said it backward in the video. Sorry!
@@CellClips thank you so much for the clarification! This subjects are so complex to me, that trying to follow is really hard, so I gotta go step by step. And that kind of took me by surprise! But your video reaaly helped me a lot in understanding this better. Bests!
Very informative and well-produced video, thank you! One question: if the Rab's are specific for different membranes, how is that specificity granted? Are the GEFs on the membranes selective for a corresponding Rab? If so, how does a target membrane get rid of the now incorporated Rab-GEFs after fusing with a vesicle that contained them to recruit their corresponding Rab?
Thank you in advance for any answers!
wow i love ur content
thanks! very helpful
very good
Isn't synaptobrevin a V-Snare? (not a T)
everybody makes mistakes, everybody has those days (Miley C. quote)
@@saarahv11 thats rude indeed
I may have messed that up, sorry.
omg i realised ur voice sounds like kevin spacey's !
I can't believe I understood this
Really interesting video but Syntaxin is a t-SNARE and not a v-SNARE, and Synaptobrevin is a v-SNARE not a t-SNARE.
that's rude people do make mistakes
@@saarahv11 Nobody's perfect!
I gotta work it!
@@saarahv11 its critic, nothing rude here....
Guy sounds like James Woods