Direct ELISA (FL-Immuno/70)

[@emanothman9665]

Itis exam night and those vid series really help

[@FrankLectures]

Glad to know this. Thank you for your feedback.

[@annettematadi5513]

Loving this. Well explained!

[@ibrahimlawan8056]

Simple and clear explanation thank you

[@FrankLectures]

Thank you.

[@parinaazfatimah1710]

Getting addicted to ur videos .... gr8 job team

[@FrankLectures]

Thanks

[@swaroopsinghjakhar3971]

Awesome presentation about immunology reaction....

[@FrankLectures]

Thanks

[@swaroopsinghjakhar3971]

The Elisa test requires
A) know Antibody
B) know Antigen
C) Patient Antibody
d) substrate
Please answer with reference
One of Rajasthan health university of science is claiming it's answer B while as per your video it's answer is A

[@FrankLectures]

I find this question incomplete. This is because ELISA test can be used to detect both antigens and antibodies.
In case, we want to detect the presence of specific antigens in the patient's serum, we should have known antibodies for this antigen (DIRECT and SANDWICH ELISA).
And in case, we want to detect the presence of specific antibodies in the patient's serum, we should have known antigen (INDIRECT ELISA).
Now, because most common application of ELISA test which we study is the HIV screening test. So, according to that, answer should be B. Because in HIV screening test, we detect antibodies (INDIRECT ELISA). Known antigens are immobilized in the wells.

[@swaroopsinghjakhar3971]

Please provide me standard reference.... Because I was participated in in exam and examiner authority declaired answer is know Antigen

[@lethabomoses7654]

Nice video. Um my question is how do we get to immobilize the antigens since they are our analyte provided that we are not sure if they are present or not in the patient's sample?

[@sulaimanolaide2036]

Yeah! How do we immobilised the Ag?
I hope we are not using Ab to immobilise it or else what will differentiate the Direct And Sandwich ELISA.
Thanks

[@zoief4088]

Very nice video. Thanks.

[@FrankLectures]

+Zoie F thank you for your feedback

[@nagwaabou-soliman2621]

thanks so much for this video

[@jasonclintespinoza1343]

so much better thanks!

[@asmaabbasi3277]

in direct ELISA we always measure antigen..??

[@donajor8]

By Elisa there are many positive igg as ebv hhv6 cmv But with negative igm. And negative pcr? What do this mean with clinical symptoms?

[@FrankLectures]

Please elaborate your question. I am unable to understand what you are asking?

[@donajor8]

On the Elisa test for ebv, cmv, hv6 the igg are positive and the igm are normal. Also the pcr for these viruses are normal. And the person is have symptoms. What to make out of this situation.

[@FrankLectures]

Sorry buddy ! Your question is out of the scope of the syllabus I have covered. Need to study more for this.

[@donajor8]

Thanks still

[@lukasbab2148]

Great video, but the Voice is awful. It is hard to keep on listening

[@mohammedelshaer6884]

does this method used for quantitative assay or just qualitative as shown ?

[@mohammedelshaer6884]

and thank you for the series.

[@FrankLectures]

ELISA is used both as a quantitative and qualitative assay. But Direct ELISA is not the preferred method. Indirect and sandwich ELISA are preferred.

[@FrankLectures]

You're Welcome.

[@mohammedelshaer6884]

how it can be used in quantitative assay if we lose some of serum (antibod or antigen) when rinsing ?

[@FrankLectures]

I don't have detailed information on this. More deep study about ELISA protocol is needed for this. Sorry, unable to answer right now.

[@asmaabbasi3277]

and in indirect ELISA we always measure Antibody..??

[@kawtarkawtar4920]

well explained but i hate the robotic voice

[@sanjaykumar-ew6mb]

Nice presentation dear madam ❤🙏🙏

[@akanequeen]

why is all biotech videos with a robot voice

[@bluegirl8058]

What about the other two types?

[@FrankLectures]

+Blue Girl other two types will be uploaded soon.

[@alokkumarsrivastava2959]

What is substrate in this?

[@inshaishrat4371]

It is the molecule or compound specific to the enzyme used to conjugate the antibody. It (complex of enz and substrate) is chosen in such a way that it gives colour after substrate is added to the mixture/ well containing the enzyme conjugated-Ab. Some examples are HRP enzyme with TMB substrate to give green colour.

[@lewispeck2236]

Is the direct ELISA technique used to detect HIV?

[@FrankLectures]

No, Indirect ELISA is used to detect HIV.

[@vasandhanm8920]

@@FrankLectures by using IgG will find out hiv?

[@samialhajaj8477]

Thank you so much

[@deryaprl9735]

Great!

[@zoisms3443]

very nice

[@FrankLectures]

Thanks

[@kelvinkisabuli7808]

THANKS

[@swaroopsinghjakhar3971]

With reference sir please

[@FrankLectures]

References for ELISA test...
1. Prescott's Microbiology
2. Microbiology, An Introduction, Tortora

[@newtonkamicha3999]

thanks

[@FrankLectures]

Thanks

[@nirnayanayak4346]

Sab marba

[@زهرهالكاظمي]

.

[@warood9690]

Thanks

[@FrankLectures]

You're Welcome

[@MedSheba]

Thanks

[@FrankLectures]

You're welcome