you're amazing! I studied my last exam from your videos and I got 17 out of 20, the teacher marked my down 3 marks for 3 questions that I didn't answer correctly because simply you didn't mention it in your videos, all thanks to you I wouldn't pass the test
Thank you for this very informative video. There is one thing I noticed though: the image on the bottom right shows the green detection antibody binding to the primary antibody with its Fc region, when actually it would bind with its Fab region. The Fab region is what gives the antibody its specificity against a certain antigen, whereas the Fc region is necessary for recognition by immune cells. Also, primary antibodies binding to two epitopes (antigens) simultaneously is dependent on the size of the epitope and (especially for IgG antibodies) not quite common. I hope I didn't create any confusion. Cheers
Yeah I was super confused by his drawing, thanks for clarifying! Also would each of the enzyme linked antibodies bind to two separate antibodies of interest because there are two Fab domains?
Love it. Thank you so much for being a kind person and explaining things for others to understand and not to show off how much smarter you are and rest so stupid. It is a rare quality in teachers to actually want to teach.
Thank you from Belgium! You are very good! PCR, Plasmids, Polyclonal Antibodies, Monoclonal Antibodies, ... all those videos are amazing! I'm not an English speaker and you talk so clear I can understand :) ... Thank you !!
That took a process that I was having difficulty understanding and made it totally understandable. Thank you so much for this and your Western Blotting video!!!!!!! My GPA thanks you as well!
Great lecture series 👍. Very clear. A minor point, in the indirect Elisa, the orientation of the enzyme linked Ab should be the same as that in the sandwich assay. It may be upside down.
Is it possible that when we 'wash' the wells, there is a chance that the bond between antigen-antibody or antibody-antibody w/ enzyme, break apart, giving us a false negative?
Excuse me, may I ask some question, if for direct detection and direct adsorption of the antigen on the wall of ELISA we still test for the presence of antibody in the blood serum or we are looking for the presence of antigen?
Great explanation of the process, but it would have been better if u added more informations about the Enzyme-linked-Antibody and the relation between the substrats catalyzation and the bond between the 2 antibodies. thank you
can the manufuctured antibody, with enzyme(green in n particular) cause change in colour incase a substrate is added to it, just in case the antibody is in its reagent bottle alone?
In terms of Indirect ELISA, how does the enzyme linked antibody bind with the antibody that is connected to the antigen? Are these antibodies able to bind through their Fc regions?
Hi Grace. Basically, WB identify proteins which are separated by molecular size and ELISA a solution of whole proteins. Also, WB could be more specific method then ELISA test which is important for confirmatory diagnostic procedures. Best regards.
Sir what is that added substrate which cause colour change..?? How does it can be wash or what is the process of remove unbound antigen or antibody..?? Plzzzz reply me sir..
Sandwich ELISA starts at 7:08
Just another big thank you from a biochemistry major for all your hard work! You're a talented teacher.
Very clear explanation and straight to the point. Thank you so much!
no worries, I worked really hard T.T.H Pham
you're amazing! I studied my last exam from your videos and I got 17 out of 20, the teacher marked my down 3 marks for 3 questions that I didn't answer correctly because simply you didn't mention it in your videos, all thanks to you I wouldn't pass the test
Sir sir sir..U r saviour of a biochemist.. Profound Respect from Pakistan...
Thank you for this very informative video. There is one thing I noticed though: the image on the bottom right shows the green detection antibody binding to the primary antibody with its Fc region, when actually it would bind with its Fab region.
The Fab region is what gives the antibody its specificity against a certain antigen, whereas the Fc region is necessary for recognition by immune cells.
Also, primary antibodies binding to two epitopes (antigens) simultaneously is dependent on the size of the epitope and (especially for IgG antibodies) not quite common.
I hope I didn't create any confusion. Cheers
good catch!
Yeah I was super confused by his drawing, thanks for clarifying! Also would each of the enzyme linked antibodies bind to two separate antibodies of interest because there are two Fab domains?
Love it. Thank you so much for being a kind person and explaining things for others to understand and not to show off how much smarter you are and rest so stupid. It is a rare quality in teachers to actually want to teach.
OMG I cant thank you enough ♡
+YulYul Addict You're welcome! :)
Lots of thanks .. you always boos our confidence. I always get excited to switch on your lectures
Thanks very much Dr, My immunology course is a always easy to understand when I listen from you!
Thank you from Belgium!
You are very good!
PCR, Plasmids, Polyclonal Antibodies, Monoclonal Antibodies, ... all those videos are amazing! I'm not an English speaker and you talk so clear I can understand :) ... Thank you !!
You are a gift. Thank you for your awesome, clear, concise and colorful explanations on these methods. :D
no you are are gift kim.
I appreciate this guy so much. Saving my grade.
The best explanations require no follow up questions. Thank you!
Thank you for this video! It must have taken a lot of effort to draw the board etc. very very much appreciated!
Great!! Very helpful to an Organic Chemist developing Biopharmaceuticals... God Bless You With More Energy!!!
I love your Teaching approach. I passed my Exams because of this online class❤❤❤❤
Just want to say that this video is SO extremely helpful. Please post more.. you explain everything so clearly! THANK YOU!
You are a living legend. Respect!
saving my gpa like always
thank you so much
Very clear! I didn't understand the Sandwich Elisa really well, but now I do! Thanks!
U did such a good job in explaining this method! Thank you so much for that explanation! (Y)
That took a process that I was having difficulty understanding and made it totally understandable. Thank you so much for this and your Western Blotting video!!!!!!! My GPA thanks you as well!
Excellent. For medical students this is a Godsend
Absolutely beautiful explanation on Sandwich ELISA! Thank you so much, this will help me with my lab reports! 🥰🥰
i love you AK lectures
Why are you such a legend?
These videos are great! You deserve more views and likes
I love your videos. They are so clearly explained. Thank you.
You're seriously underrated! Thank you so much!
the opinion of the scientific community is that he is appropriately rated
AK lectures are the best....best explanation to every topic...thank you for making such helpful videos...can't thank you enough😊
Great lecture series 👍. Very clear. A minor point, in the indirect Elisa, the orientation of the enzyme linked Ab should be the same as that in the sandwich assay. It may be upside down.
Is it possible that when we 'wash' the wells, there is a chance that the bond between antigen-antibody or antibody-antibody w/ enzyme, break apart, giving us a false negative?
Watch this on the day of your exam….I see you!
Staying up late trying to understand this
Keep going!
THANK YOU , THANK YOU , THANK YOU!!! GOD BLESS YOU!!
Excuse me, may I ask some question, if for direct detection and direct adsorption of the antigen on the wall of ELISA we still test for the presence of antibody in the blood serum or we are looking for the presence of antigen?
Anyone know if all his biochem videos cover all the MCAT biochem topics? Please help!
Thank you so much! I loved the way you explained all the concepts
Wow you are such a good explainer and teacher.... Keep up the good work... Kudos to your explanation
I love you. You're videos are so helpful. I wish i could just take your class
he might not be able to wear that sexy athletic-cut v-neck in his class though, so you may be better off!!
You're amazing! Thank you for making this simpler.
thank you so much all the other videos I watched were so confusing but you made it so understandable!
Great explanation of the process, but it would have been better if u added more informations about the Enzyme-linked-Antibody and the relation between the substrats catalyzation and the bond between the 2 antibodies.
thank you
Thank you for uploading this! Very concise and helpful!
can the manufuctured antibody, with enzyme(green in n particular) cause change in colour incase a substrate is added to it, just in case the antibody is in its reagent bottle alone?
Thank you very much for this explicit lesson!
Omg im so glad i found your channel. Thank you!
Thank you so much for this clear explanation!
Thank you very much, so well explained and understandable.
Thank you so much for this perfect explanation
In terms of Indirect ELISA, how does the enzyme linked antibody bind with the antibody that is connected to the antigen? Are these antibodies able to bind through their Fc regions?
how is the first antigen or antibody we added at the first not removed when we wash off after each step ??
thanks be to AK lecture ,sir you forgot to add stop solution especially HCL acid .but actually explanation was very fine.
So clear and concise! Thank you!!!
very helpful and clear as usual one question though I'm not seeing Direct and Competitive ELIZA did I miss it?
Killed it bro! Thanks !
I spent the whole trying to understand this concept . just after I watched your video everything became clear ,thank you so much
Wow ! This was very informative thank you
the best explanation ever! thank you
Awesome explanation!
Thank you so much it helped me for exams
This video is just what i needed! thank you
Excellent lecturer!
U r the best 💜🇮🇶🥀
i love your lectures so much thank you!!!!
If I have the following solution: 3x capture antigen (dilute with 1x PBS). Does this mean that I have to dilute it or is it already diluted?
one of the best Videos
Brilliant!! Thank you. So clear.
O my Goa! Really you are agreat lecturer!!!
Isn't antibodies is present on the eliminated body
Thank you for your help!!
Thank you ❤
Great explanation now it’s clicking
Thanks very much for this helpful video.
i’m taking A level biology, and this was confusing but thank you for this video!!!
taking this again in med school, and i came back to your video… thank you so much!
That's very helpful, thank you so much. Could you please explain about competitive ELIZA🙏🏻
Excellent
Jazak ALLAH sir❤️❤️❤️
Simple way thanks
But what about direct and competetive eliza
You are the best! Thank you sooooooo much
Saved me again. Now im ready for my exam tomorrow!
😂😂
Sir your oldest student, I passt my many tests bcz of u
So what exactly is the difference of ELIZA and Western Blotting if theyre both used to identify proteins?
Hi Grace. Basically, WB identify proteins which are separated by molecular size and ELISA a solution of whole proteins. Also, WB could be more specific method then ELISA test which is important for confirmatory diagnostic procedures. Best regards.
I finally got it right! Thanks!
You are the best really🦋
Thank you so much for your videos!
You sir are a legend. Thanks a lot.
no, you madam are a legend
thanks for the explanation helpd me alot !!
Sir, YOU ARE AMAZING, thank you so much! :)
thanksss ! very useful and detail explanation
Sir what is that added substrate which cause colour change..?? How does it can be wash or what is the process of remove unbound antigen or antibody..?? Plzzzz reply me sir..
Amazing explanation
Thank you so much for this very informative video :)
Thanks a lot.. Very very helpful..❤
Very clear explanation ☺
Thanks a lot! You are the BEST ever! I just donated:-)
elisa for s sorbent !!! u wrote eliza .. :) luv ur videoss................thankuu
ربنا يكرمك يا حاج والله 😂❤❤❤
you've helped me very much ,, thank you
Very clear , thank you a lot !