I pay thousands of dollars every semester to be taught by so called "experts" in their field, but at the end of the day i'm learning most from fucking free online resources. smh.
being an expert and being able to teach is two separate topics. I feel that many of the professor's primary goal is to research rather than to teach. I attend prestigious research university and I often hear professors boasting about how they contributed to further our knowledge while giving low quality lectures. I hope there were some kind of system where they only hire dedicated and qualified full time teachers who is not necessarily the most bright expert on the cutting edgy of the knowledge.
You are the only one who could explain each step! Other youtubers and even my teacher left out how exactly a ddntp stopped the building of complementary DNA strands. Thank you!!
01:18 What particular enzyme does this? 01:45 So how to turn a "normal" 2'-deoxy nucleotide into a 2',3'-di-deoxy nucleotide in a beaker? Is there some enzyme involved as well? Or just an ordinary "inorganic" reaction? How about the fluorescent dyes used to mark them? What chemicals are they exactly? 07:00 What if there are some gaps because no ddR happened to attach at that particular site? Shouldn't there be additional row in the electrophoresis process with a DNA ladder to mark the places of all possible lengths for reference?
Quick quention, I watch other videos on the same topic however them mention that the new dideoxy bases had 3 phosphate groups attched instead of the usual one, besides that I completely understand, thanks a bunch
thank u thank thank u thank u .. it's a great explanation i totally gets it now! .. pleaaaase do more videos about different topics of molecular genetics or biochemical reactions or any other methods! that would be great and helpful .. im subscribing already! ;)
But i think you should have mentioned that this seq. we optained is a 5'-3' seq. and its only a complimentary seq. to the original template we had (3'-5').
Hi there, thanks for your video, it's really well explained. Still, i don't get how it is possible to know what's the last nucleotide. Could you provide more information? Thanks in advance!
but how can I know what is the order of the nitrogenous bases before the dideoxy nucleotide thingy?! I mean yes I can know how many nucleotides there are, but how can I identify their identity?!!!
+Sarah M.T. Well, you've got one lane on your gel that has dideoxy A and regular deoxy G, C, T. So in that lane, you will get a bunch of fragments of different lengths. Each length fragment represents a position in the DNA where A occurs (because synthesis would only end when a dideoxy A is introduced). It doesn't matter what sequence comes before that position; you just know that there's an A there. Follow that same logic for every fragment in that lane and you know the position of every A in the sequence. Follow the same logic for the other lanes and you can deduce the position of every C, every G, and every T in the sequence as well. Once you've got the positions of every A, C, G, and T, that means you can deduce the full sequence.
I pay thousands of dollars every semester to be taught by so called "experts" in their field, but at the end of the day i'm learning most from fucking free online resources. smh.
+SuperMmmm33 So true
Well said :D
You're paying for the college credit...not the knowledge :)
being an expert and being able to teach is two separate topics. I feel that many of the professor's primary goal is to research rather than to teach. I attend prestigious research university and I often hear professors boasting about how they contributed to further our knowledge while giving low quality lectures. I hope there were some kind of system where they only hire dedicated and qualified full time teachers who is not necessarily the most bright expert on the cutting edgy of the knowledge.
Lol well professors are more into their research rather than their students unfortunately 😔
you are the only one who explained in a way I could understand every step. Thanks!
I just watched a 1hour long video of this subject and you just said everything in it in 8min
THANKS
i love the stopping sound effect. Thanks for the explanation! Showing the chemical structures really helped!
You are the only one who could explain each step! Other youtubers and even my teacher left out how exactly a ddntp stopped the building of complementary DNA strands. Thank you!!
That was the absolute perfect explanation. I'm sending this video to my friends who are in the same Biology course. Much appreciated!
explains more than what I have read for hours. excellent and thanks!
This helped A TON, it was the clearest video I could find.
thanks a lot! even it's in english i had no problems with understanding - great video!
Thank you sooooooooooo much! The thorough explanation really helps with understanding the experimental process/results. Can't thank you enough!
Thanks! Much clearer now! Other videos on youtube do not give this step by step explanation, so it was really helpful!
i swear you are better than our professor !!
Such a good video, got my biology exam tomorrow!
I'm tired of having my teachers not being able to teach as well as online videos teach me
education of the future my dude
Your teacher would be just as good if it was one-to-one and you had a pause/rewind button.
seriously best video ! there was a link to it in our lecture ! THANK GOD
To the guy who made this beautiful video: YOU ARE AMAZING
Thank you for the teaching of Dideoxy DNA Sequencing.
Bless you.
Big Thank you Dr.Johnny ,,you save my life
great video!!! I read my book and didnt understand it. this video made it so clear!!! thanks
THANK YOU YOU MADE THIS CLEAR AS AN INVISIBILITY CLOAK!!!
This is the only video that made it click. Thank you
This explains it so much better than my book, thank you!
the best vid here regarding this topic!
this method is just genius! thanks for the video
Fantastic Video!
You are a good teacher!
01:18 What particular enzyme does this?
01:45 So how to turn a "normal" 2'-deoxy nucleotide into a 2',3'-di-deoxy nucleotide in a beaker? Is there some enzyme involved as well? Or just an ordinary "inorganic" reaction?
How about the fluorescent dyes used to mark them? What chemicals are they exactly?
07:00 What if there are some gaps because no ddR happened to attach at that particular site? Shouldn't there be additional row in the electrophoresis process with a DNA ladder to mark the places of all possible lengths for reference?
You made it so easy to understand.Thanks.
thanks for your best lecture .i really understood this from your awesome presentation.thanking you so much
Thanks for such a great educational and well-explained video.
this one so far is absolutely the best video thanks for saving my ass before my final Adv biology exam :)
Thank you. Better than my uni lecturer.
Great job explaining, it made everything very clear!
It makes so much sense now!
clearest vid i could find, thanks a tonne!!
0:27
Note that there is a double bond between the C5 and the C6 atoms in the thymine base.
Quick quention, I watch other videos on the same topic however them mention that the new dideoxy bases had 3 phosphate groups attched instead of the usual one, besides that I completely understand, thanks a bunch
thanks for the video...A good quick guide and refresher....
thank you so much for this presentation! it's explained fantastically! I finally understand it, thank you very very much!!
Finally understands this... Thank you so much!
Very clear explanation! Thanks!
thanks a lot it is the basic and simple way to understand
Now I get it. Thank you so much! Life saver!
Actually, really good explanation!
fantastic explanation!!!
this was a perfect video! subscribed!
Great explanation, thanks!
thank u thank thank u thank u .. it's a great explanation i totally gets it now! .. pleaaaase do more videos about different topics of molecular genetics or biochemical reactions or any other methods! that would be great and helpful .. im subscribing already! ;)
thanks .... very goooooood explanationn........... it was so helpful for me......
this is too awesome! thanks a bunch for clarifying so much...
HERO, thank you so so so much!!!!
thank you soo much i now get it excellent explanation. Please do you have one on pyrosequencing?
But i think you should have mentioned that this seq. we optained is a 5'-3' seq. and its only a complimentary seq. to the original template we had (3'-5').
Great video!
This made more sense than my book.
I'm ready for my test now ;D Thank you!
Brilliant video; thank you
Please what can happen if the report of ddNTP/dNTP increase or decrease ???
Hi there, thanks for your video, it's really well explained. Still, i don't get how it is possible to know what's the last nucleotide. Could you provide more information? Thanks in advance!
Awesome video
love this video..thanks a lot!
Perfect lecture!
Can you send me a copy of the powerpoint to help me teach others about this?
perfect explained
nicely done
thx
Thanks, great video
and i wish if u give us a link 2 download ur PPT show!
that was awesome loved it...u simply forced me to comment
thank you...it is very clear!!
This is amazing
thank you very much.now it is clear..
Thank you for the upload. The whole process seems like its very lengthy.
Thank you!
Thank you SO much
Exam will be easy now thank you
you earned a Subscription my friend :)
thanks great explanation
thanks, this is perfect
very helpful !
Thanks!
Thank you
but how can I know what is the order of the nitrogenous bases before the dideoxy nucleotide thingy?! I mean yes I can know how many nucleotides there are, but how can I identify their identity?!!!
+Sarah M.T. Well, you've got one lane on your gel that has dideoxy A and regular deoxy G, C, T. So in that lane, you will get a bunch of fragments of different lengths. Each length fragment represents a position in the DNA where A occurs (because synthesis would only end when a dideoxy A is introduced). It doesn't matter what sequence comes before that position; you just know that there's an A there. Follow that same logic for every fragment in that lane and you know the position of every A in the sequence. Follow the same logic for the other lanes and you can deduce the position of every C, every G, and every T in the sequence as well. Once you've got the positions of every A, C, G, and T, that means you can deduce the full sequence.
Thank you very much! :D It´s finally clear! :D
thank you!!!
buenisimo!!! thanks!
Helpfull, thank's !
THANK YOU SO MUCH :")
THANK YOU! :)
Thank you =)
Awesome
thanks dood
thanks :D
Molecular Biology rocks
thankyouuuuuuuuuuuuuuuuu
woooow you smart piece of shit, i love you soo much man
this information is too simple
I like potato!
Thank you!
Thank you so much.
Thank you
thank you