Sanger sequencing
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- เผยแพร่เมื่อ 2 ต.ค. 2019
- This is an animated video on DNA sequencing by Sanger's method.
Sanger sequencing method is based the use of dideoxy nucleotides in the DNA polymerizing reaction. The hydroxyl group attached at the 3' end plays a very important role in DNA polymerization reaction
during DNA polymerization, There is a reaction between 3'OH group and the phosphate of the new dNTP to be added.
in 1977, Frederick Sanger had a brilliant idea. if the 3'OH group of the nucleotide is absent, then there wont be addition of new nucleotides and the reaction would stop. The incorporation of dideoxy nucleotide and termination of polymerization reaction is a random process. hence the overall mixture will have several fragements that have experienced the chain termination. If the length of each fragment is known then the location of nucleotides in the DNA can be estimated.
Classic example of some random youtube dude better explaining a concept than my professor can
funny how my professor used the video of this random dude to explain it to us
So do I 🤣
You are from which country and which college ,friend
True af😂
God bless you you're right dude...
Its crazy how things like this when taught correctly are so easy to comprehend. Professors/Teachers need to take notes from this guy!
That is 100% true. The video animation and a carefully thought-out presentation enables a good teacher to reach millions of students on TH-cam.
crazy a 6 minute video is more helpful that 1.5 hr lecture...
To be a good teacher, you also need to know how to talk. This guy failed at that, his english is so poor that you do not even know what he is saying.
tf ur talking about his english is great@@m.m.3552
Just because you have a PhD in something, DOES NOT mean you are a good teacher. Thank you so much for this my friend.
this is the best video on Sanger's Method I've seen. I finally understand it now. Thank you so much!
Me too!
i have done it at least 1000 times and i wouldn't be able to explain it better
Yes! Totally Agree! Simple and Comprehensive!
Yeah, simple best
My goodness, people really weren't joking when they said you're an amazing teacher. Aside from having my mind blown from how genius this technique is, my mind is also blown from how amazing of a teacher you are. Thank you ever so much!!!
i just want to thank you, you explained this better than most of my professors during my pharmacy school years. thank you!
Best video on TH-cam available for sanger sequencing.
This is hands down the BEST Sanger's Method video I've seen. So clear. Excellent work! Thank you
The most clear and thorough explanation of Sanger Sequencing. I couldn’t wrap my head around how the sequence of the strands are determined but it is thoroughly explained in this video
I have been struggling to understand this concept, and this video was perfect!!! Thank you very much.
I mean my professor made this topic so hard that I thought I don't have the brain cells to understand this. Tysm buddy
Saved my life, i hate not understanding something but thankfully i found this video. Great explanation!
This is one of the best science concept explanation videos I've seen on youtube in a while. Thank you for explaining this concept interestingly.
My Microbiology exams are approaching and this video is a pure Bliss to me.This video is perfectly making my bases strong along with making me understand the whole topic which my professor failed to during a whole 1 hr lecture in my native language.
This is my go to video to brush up on Sanger's method. Thank you SO much for this!!
this 6 minute tutorial saved me a full hour of time of watching my lectures, thank you.
Clear, helpful, and straight to the point presentation. Well done and Thank you!
THis makes a confusing mess out of this. Again Shomu has the best explanation on the web. This just makes something that should be simple into a mess which is something that biologists are very good at.
Very explicit! I wish I could like this video a million times. Thank you so much!
I owe you my firstborn child and my left testicle. Thank you for your services I am now enlightened
Very clear and easy for understanding! Thank you so much!
Thank you. Have been going nuts trying to understand my uni lecture and other you tube videos until I found this one.
It's insane this works for whole genomes! Excellent explanation!
This was so very well explained. Thank you!
Exactly what i was looking for. Thank you!
This is really the beeeeest video about sequencing method . very simple, very clear, wonderful!
U just killed it ...rather than watching so lengthy and complex videos ..its far better and easy way to learn
:-)
The most amazing explanation! Simple and perfect! Thanks
Thank you! Very helpful lecture and an amazing explanation as to how the procedure works.
This was amazing, especially for My MCAT prep !!! cleared up so much , thank you
had to watch it twice but this concept finally clicked for me, thank you!
thank you so much for a clear and detailed explaination! Your work is much appreaciated!!!
Very nice, simplified way of explaining. Very easy to understand. Thanks
That was amazingly Explained in a very simple manner .Thanks Dude.
your explanation is perfect thank you so much
Thank u soo much ..teachers will hurry to finish their chapters and nothin would enter my brain..thanks for this video
You are the best explainer ever ❤❤
So far, this is the best video explaining this topic!
that was so concise and to the point thank you !
Easily the best explanation of Sanger's method. Thumbs up
Phenomenal explanation! Thank you for this video it helped a lot.
This was so clear, thank you so much!
Explained so nicely, and the technique itself is so brilliant ❤
Fantastic video! The information was presented in such a clear way that it really helped!
This is called a perfect vedio. Just amazing ♥️ I'm really amazed how someone can make us understood this hard topic so easily within only 7 minutes
YOU ARE THE BEST!! thankkk yoooouu vary vary much from deep inside for this amazing video.
Absolutely fantastic explanation. The explanation of the x-ray film method and the evolution into the electrophoretic capillary method was unclear to me in the textbook, so this saved me a great deal of time. :)
Thank you so much! I found this video very informative
Thank you for your well clear explanations and well animated video.
So well explained. Thank you!
brilliant, thank you for making this video, best explanation on youtube
Thank you very much!!! The best explanation for Sanger sequencing💌
Thank you! You explained it super well!
Thank you! That was very helpful 😊
Thank you making it easier and more understanding 😌
way better than my prof explaining... thanks so much
Thanks for this video, it's the best one! Thank you so much!
Thank you for this illustrative video I totally get the concept
Exceptional presentation. I can breathe again.
Great simple explanation! thank you very much
Very well explained, thank you very much!
Never understood this except for now..... Amazing !!
You're a star thank you for explaining this so well!!!!! :D
This clarification is sooooo cool!!! Big thanks
So much Understanding God bless you
i love this video, my teacher spent 1,5 hour explaining this and i couldn't get it, now I finaly understant
The best video ❤ Soo easy to understand and all the details are covered.😇
That was super clear and helpful !
Thank you for the explanation! Easy to follow and understand! :D
I could learn this I my sleep, so easy to follow
Now this is amazing explanation.
an absolute true legend hands down.
You explained this better than my teacher. Really nice video
very clear and concise; Thanks a lot
What a great video. I understood everything
This is a fantastic short and simple video :)
that was life changing! I hope I will use all that information on my test tommorow. I want to say hi to my dear friend Dawid
I also want to thank you because i and my dearest friend Szymon will kick some asses on tomorrow exam
Hey you deserve a Nobel in education!
very clear explanation! thank you!
Such a beautiful class and good explanation, thankyou ❤
It was super clear, thank you!!
Thx a lot Mr. "I will teach you, my son".
Wow I have my biochem exams in 5 hours and this is a life saver
Exactly what I was searching for... 😍🙏👌👌👌
the best video, thank you so much man
Finally i got best explaination on this topic thank you very much ☺️
This video is so informative it sort out my problem in this topic
Your video helped a lot hope you are continuing making this kinds of videos
So cool!! Great explanation, tysm
Thank you! That was very helpful :)
What a great video! thanks a lot!
amazing presentation. thank you for this.
Bro my dev bio professor cannot explain this sh*t in even a remotely comprehensible manor lmao. Thanks.
I needed this. Thanks
Thank you so much, easiest explanation ever got 👍
Great video! Thank you
So happy to see this video..it is much informative
Thanks. This video helped me a lot.
Perfect job at explaining 👌
Very clear explanation. Thank you
thank you so much. it was so clear