Congratulations from México you helped me and my girlfriend to understand many of our Mollecular Biology lectures. You definitely have the gift of teaching. Thank you very mucho. "Eres un chingón" as we say here in México
I'm from Chile, and you are make me pass Molecular Genetic with your videos!! Thank you!! I appreciate that you make, sorry for the bad english, but I understand you more than my teacher jajaja.
Very helpful video.... thank u so much...for this video....it's easy to understand the DNA replication....I was not so interested to understand this because it's very difficult but due to your video I have much more interest in it.....😍😍😍😍😍
Thankew sir...u helped me a lot through all your molecular lectures...for only u I clearly understood the mechanism of central dogma...and I am able to do many questions from central dogma...thankew a lot...
Sir jo apne pehle vale lecture mei btaya tha ki polymerase alpha primer bhi add krta hai and some deoxyribonucleotides bhi....?sir yha pe doubt hai please make it clear....
Sir what about the tau protein there is no information given by you in the lacture.... Sir please tell me about it. You can tell me in in the comment also...
do you have any idea why we dont need a clamp during regular pcr? Is it due to unique abilities of commercial pol enyzmes or relatively shorter length of desired pcr product?
The action of DNA pol epsilon is not thought to be used in the leading strand. DNA polymerase delta is used in both leading and lagging strand after the action of pol alpha, as it is more processive than than polymerase alpha and has proofreading activity. DNA pol epsilon is thought to only be used in the lagging strand though it's use is unclear!
@@niharikasingh5551 never trust non-science websites for scientific details as they are most likely not written by those with any science knowledge and so can be incorrect.
@@jenythakkar9319 Yes you are correct. I should not have quoted about Wikipedia... 1 year ago I didn't have much resources and I was just beginning to study genetics... Today I rechecked about DNA polymerase epsilon in the 'MOLECULAR BIOLOGY OF GENE' by JAMES D. WATSON and cleared my doubt...Chapter 9, page 278, 2nd para , 5th line....clearly mentioned about DNA POLYMERASE EPSILON Thanks... well can you suggest me some good scientific websites🙏 I have to make my own notes and complete my assignment
@@niharikasingh5551 Hey, I've heard Mr Watson somewhere and I'm glad you have somewhere you can rely upon for in depth information. Currently, if there is anything I want to study outside my syllabus, and normal websites aren't helping, then try reading the most recent articles published by pubmed or scopus. You can find a lot of in depth information there. It's a lot to read definitely and sometimes I really can't be bothered but it's definitely worth it!
Video 3, Day 3. Everyday I have planned to watch at least one video from ur channel. But I can't sir🙁. But I'll do nd learn something from your channel sir.
Hi Sir! Thank you so much for your helpful videos. I have a question regarding the replication loop. In the picture you have drawn it seems like the leading strand in the replication bubble is moving the opposite direction of the newly created replication loop, where is the second fork located and how is it connecting the leading strand with the looped lagging strand?
oe oe...i understood now both of the strands wereparental strand..but even then having confusion there and that is the new strands formed according to your structure are moving not according to the sequence of 5' to 3'
I've been trying to understand which DNA polymerase attach at which strand for hours now and wherever I go, I get different answers. You are saying that DNA polymerase epsilon goes along the leading strand and DNA polymerase delta goes along the lagging strand. However, some websites are saying it the other way around!? Whilst other websites are saying that DNA polymerase delta works on both the leading and lagging strand and that DNA polymerase epsilon acts as a proof reader. I'm so confused! What's right?
There will no folding of dna strand to continue the polymerization as in prokaryotes ? And in prokaryotes u told that polymerization will start from the end but in eukaryotes u said it will start from the bend or curve that u have drawn in diagram plz clear
Thanks for the great work mate :) it helped a lot I was reading the harper's illustration for biochemistry and your videos made it much more understandable Thanks once again
Sir, as u said few DNA sequences are added by pol alpha before pol delta is recruited.....so whether we need as many pol alpha as RNA primers?? Because as pol delta falls....it is goes to another primer with hydroxyl grp to initiate.
DNA pol alpha will act after each primer (so after every primer in the lagging strand and the first primer in the leading strand). This is because, DNA pol delta and epsilon can't synthesize new DNA straight after an RNA nucleotide in the primer. And DNA pol alpha can't continue with its DNA synthesis because it is not highly processive (it has no proof reading activity) and so is error prone and to have DNA pol alpha to synthesize long strands of DNA can be highly mutagenic. Therefore, after producing a short span of DNA, DNA pol epsilon or delta need to take over because they have good proof reading capability.
Ur strand direction in the figure is exactly opposite of what it should be... It created a lot of confusion.. regarding the primer and dna pol alpha attachment. Also the dna delta is on leading strand.. and dna epsilon is on lagging strand
Sir I have questioned that can telomeres is seen on leading strand ?because there is also 5'-3' and after removal of primer there is no 3' hydroxyl group.right or wrong sir?
@@devanggondaleeya4321 That's a very good question! I just drew out a few diagrams to explain everything and I think you are right. You do get telomeres at the lagging and leading strand. On the leading strand, you would get the telomeres at the 5' end on the lagging strand, you would also get the telomeres at the 5' end.
Sryyy sir but I couldn't understand what is the need of looping in eukaryotes if there r two different molecules for polymerization also they r not starting from the points as it was needed in prokaryotes as there was single polymerase molecule for polymerization.also in the lagging strand u told it works in back stitching manner what we hv learnt in previous classes (11th) without looping.
Sir u r superb. Bt at one place u told that DNA delta when reach at Rna primer it falls off and then again go back ...which makes clear that it terminate nd do not polym.. further at rna primer...after that u told abt DNA epsilon on leading strand... then again u say that DNA delta starts cleaving RNA primer when it reach there... so this point is confusing...
Sir, you have not upload termination part of DNA relpication in Eukaryotes. Plz upload. your explanation is very nice and simple. Thanks Sir. Plz upload Termination part.
You r d gift from Allah ...for d Kashmiri students coz here d institutions r mostly closed
Thank you so much for appreciating my efforts
4 yrs old video.. But still it is more clear than any other books... Best teacher.. Stay blessed Sir. ❤❤
Thank you so much for appreciating my efforts
You are a GEM...one of the finest lecturers in this life science field....Best wishes from a Student of Kolkata...
Thank you so much for appreciating my efforts
Welcome 😄
I understand shomu's biology. Thank you sir. God bless you!
Congratulations from México you helped me and my girlfriend to understand many of our Mollecular Biology lectures. You definitely have the gift of teaching. Thank you very mucho. "Eres un chingón" as we say here in México
Thank you very much for appreciating my efforts. Please stay tuned
That's nice
The way you explain just outstanding...
My pleasure to have a teacher like you........
You are hope for me sir.....
My concept became crystal clear due to you
You're welcome. Glad to hear that you're getting benefit from my lectures
Thank you shomu's biology u save my molecular biology paper ❤
Glad to hear that you're getting benefit from my lectures
I'm from Chile, and you are make me pass Molecular Genetic with your videos!! Thank you!! I appreciate that you make, sorry for the bad english, but I understand you more than my teacher jajaja.
Thank you very much. Glad you like my lectures. Do subscribe.
I approximentaly saw all video of him . He explain biology event with high level of information . I really appreciate you. Thank you for videos
Very clear explanation. Thank you for this vedio.
GINS is a abbreviation by taking the first letters Japanese numbers 5-1-2-3 ( Go - Ichi - Ni - San )
Very helpful video.... thank u so much...for this video....it's easy to understand the DNA replication....I was not so interested to understand this because it's very difficult but due to your video I have much more interest in it.....😍😍😍😍😍
Glad to hear that you're getting benefit from my lectures
U defnitely reach higher level
Thank you. Glad you liked my lectures
It's really the best understanding video lecture of Replication in eukaryote 👌👌👌for me.Bless me Sir For my Tomorrow exam
You're welcome. Glad to hear that you're getting benefit from my lectures
Dada thank you so much,tomar moto jeno teacher hote pari..Nd you are improving day by day..First er tumi r akhn er tumir moddhe onnek parthokko..🍁
you have an impressing way .exeptional work.God bless you
seriously sir u r great, beacuse of u I'm getting understand clearly 😇😇😇😇 keep going sir
Thabk you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures
The length of okazaki fregment is 1000-2000 in prokaryotes.nd 100-200 in eukaryotes..
U say that the fragments are long in eukaryotes..
Sorry. Made mistake. Yes you are right
Thank you sir...your videos helped me a lot nowadays... Especially Molecular biology and immunology videos...
You're welcome. Glad to hear that you're getting benefit from my lectures
u r very best teacher........... the way u explain is awesome..........
Thank you. Glad you liked my lectures.
:-)
Very nicely understand video sir thanks.....and helpful for microbiology God bless you 😊😇😇
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures
Thankew sir...u helped me a lot through all your molecular lectures...for only u I clearly understood the mechanism of central dogma...and I am able to do many questions from central dogma...thankew a lot...
Glad to hear that you are getting benefit from the videos
very well explained... helped a lot before reading any standard book
+sweet Firdaus thank you. Glad you liked my lectures
U r just awesome , ,,,,, love ur videos ,,,, best teacher ever
You're welcome
Sir jo apne pehle vale lecture mei btaya tha ki polymerase alpha primer bhi add krta hai and some deoxyribonucleotides bhi....?sir yha pe doubt hai please make it clear....
Animation videos couldn't make me understand this even after watching so many videos. But you did it 🙏 you're next to god for us science people
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures
Very very nice and
INFORMATIVE
Thank you sir😊🌟🌟🙏🙏
You're welcome. Glad to hear that you're getting benefit from my lectures
@@shomusbiologyofficial 😊
Amazing explanation😇...easy to understand ...and..one of the best video
Thank you so much for appreciating my efforts
Really sir am a huge fan of your lecture
Thank you
Sir what about the tau protein there is no information given by you in the lacture.... Sir please tell me about it. You can tell me in in the comment also...
do you have any idea why we dont need a clamp during regular pcr? Is it due to unique abilities of commercial pol enyzmes or relatively shorter length of desired pcr product?
Thank u so much sir ..this video is very helpful in understanding the concept
You're welcome. Glad to hear that you're getting benefit from my lectures
Thank you again and again sir...
You're welcome
thank u sir....the way u explain is clearly understable
+sukruti pal thank you. Glad you liked my lecture.
I appreciate your videos, I liked them all, kindly provide with termination part please, my humble request
+Chandni rathi I will add that soon
May u live long vry help ful vedio thnks
You're welcome. Glad to hear that you're getting benefit from my lectures
Thanq very much. It helped me a lot.
Uve explained so well. But How is the rna primer from leading strand removed??
Lagging strand primer removal is explained.
Plz reply
The action of DNA pol epsilon is not thought to be used in the leading strand. DNA polymerase delta is used in both leading and lagging strand after the action of pol alpha, as it is more processive than than polymerase alpha and has proofreading activity. DNA pol epsilon is thought to only be used in the lagging strand though it's use is unclear!
yeah same question is here
please do check Wikipedia...it is clearly mentioned that polymerase epsilon is used in leading strand
@@niharikasingh5551 never trust non-science websites for scientific details as they are most likely not written by those with any science knowledge and so can be incorrect.
@@jenythakkar9319 Yes you are correct. I should not have quoted about Wikipedia...
1 year ago I didn't have much resources and I was just beginning to study genetics...
Today I rechecked about DNA polymerase epsilon in the 'MOLECULAR BIOLOGY OF GENE' by JAMES D. WATSON
and cleared my doubt...Chapter 9, page 278, 2nd para , 5th line....clearly mentioned about DNA POLYMERASE EPSILON
Thanks...
well can you suggest me some good scientific websites🙏 I have to make my own notes and complete my assignment
@@niharikasingh5551 Hey, I've heard Mr Watson somewhere and I'm glad you have somewhere you can rely upon for in depth information. Currently, if there is anything I want to study outside my syllabus, and normal websites aren't helping, then try reading the most recent articles published by pubmed or scopus. You can find a lot of in depth information there. It's a lot to read definitely and sometimes I really can't be bothered but it's definitely worth it!
hi I think polymerase epsilon does not require PCNA
hi u r doing a great job. thanks I m learning a lot from videos. I m NOT able to find eukaryotic termination. can you help me please.
Video 3, Day 3.
Everyday I have planned to watch at least one video from ur channel. But I can't sir🙁. But I'll do nd learn something from your channel sir.
I am honoured
Fantastic considered sir
Hi Sir! Thank you so much for your helpful videos. I have a question regarding the replication loop. In the picture you have drawn it seems like the leading strand in the replication bubble is moving the opposite direction of the newly created replication loop, where is the second fork located and how is it connecting the leading strand with the looped lagging strand?
i have searched about the loop which you have talked about but can't find it... please help me out...provide me the data regarding this
So there is no loop formation like prokaryotic so that both leading and lagging goes in same direction?
oe oe...i understood now both of the strands wereparental strand..but even then having confusion there and that is the new strands formed according to your structure are moving not according to the sequence of 5' to 3'
u r awsome suman sir................................best explanation....
+Anam Fatima thank you. Glad you liked my lecture
let me know how the primer of leading strand removed and how that gap is filled after removal of primer?
The final video ie... termination of eukaryotic DNA replication is not there.......
Sir wonderful job..1 thing i want to clear..the okazaki fregments are more in prokaryotes than in eukaryotes..please confirm this..
Awesome sir..
Sir, what is the production of DNA flap in lagging strand during the removal of RNA primer ?
sir can u please give the link of termination of dna replication in eukaryotes....please sirrr
I've been trying to understand which DNA polymerase attach at which strand for hours now and wherever I go, I get different answers. You are saying that DNA polymerase epsilon goes along the leading strand and DNA polymerase delta goes along the lagging strand. However, some websites are saying it the other way around!? Whilst other websites are saying that DNA polymerase delta works on both the leading and lagging strand and that DNA polymerase epsilon acts as a proof reader. I'm so confused! What's right?
Which protein or enzyme cleaves the primer in leading strand?and which pol fill the gap ?
part 4 eukaryotic DNA replication is not available there
Please tell me the books and author's name from which you are reading this it'll help me a lot plzzz reply ..
There will no folding of dna strand to continue the polymerization as in prokaryotes ? And in prokaryotes u told that polymerization will start from the end but in eukaryotes u said it will start from the bend or curve that u have drawn in diagram plz clear
where is video 4
cant find it?
Thanks for the great work mate :) it helped a lot
I was reading the harper's illustration for biochemistry and your videos made it much more understandable
Thanks once again
Really super I clear all this pcna and legging strand Sir txxxx
You're welcome
Sir, as u said few DNA sequences are added by pol alpha before pol delta is recruited.....so whether we need as many pol alpha as RNA primers??
Because as pol delta falls....it is goes to another primer with hydroxyl grp to initiate.
Thankyou...!
Things made EASY.
Helped a lot...!
+Craving Curiosity thank you. Glad you liked my lectures
Thank You Sirrr......
You're welcome
Thanks a ton sir❤️🙏
DNA polymerase e is involved in DNA
synthesis on the lagging strand not on leading strand.
is PCNA ring a beta clamp?
Yes
Yes.. The 3D structure of PCNA is remarkably similar to that of beta subunit of E.coli DNA polymerase III.
Oh you are soo cute....😘😘 I love ur lectures.
+Anmol Gaimukte glad to hear that. Please subscribe and share
great explanation keep up the good work love your videos
Superb
Sir did fen 1 or dna 2 also used for leading strand..
Yess...
Sir,Will pol alpha acts along with all the primers in the lagging strand or only with the first primer?
I'm too confused abt this....
DNA pol alpha will act after each primer (so after every primer in the lagging strand and the first primer in the leading strand). This is because, DNA pol delta and epsilon can't synthesize new DNA straight after an RNA nucleotide in the primer. And DNA pol alpha can't continue with its DNA synthesis because it is not highly processive (it has no proof reading activity) and so is error prone and to have DNA pol alpha to synthesize long strands of DNA can be highly mutagenic. Therefore, after producing a short span of DNA, DNA pol epsilon or delta need to take over because they have good proof reading capability.
@@jenythakkar9319 thank you
polymerase delta is on leading strand
polymerase epsilon on lagging strand
no
so what is right answer
FDDA THOMAS bhai sahb aap wrong ko right nhi bta skte or se toh basic hi glt bta rhe h...
Pol epsilon on the leading strand and delta on lagging strand..
Pol € synthesis on leading strand
Pol delta synthesis on lagging strand
However these role can be reversed
Plz make video on structure and function of polymerase
Okay
you are talking about leading and lagging strand but you drawn the single parent and daughter strand ..m got confused here help me out..:(
can you please upload termination in DNA replication in eukaryotes
What is the direction of replication fork? Is it fixed?
Hey brother great video
I wanted to know about how the nucleotides are charged i.e. how are nucleoside triphosphate formed
where is the 4'th i.e last video (termination in eukaryotes) in this series ???? pls
Ur strand direction in the figure is exactly opposite of what it should be... It created a lot of confusion.. regarding the primer and dna pol alpha attachment. Also the dna delta is on leading strand.. and dna epsilon is on lagging strand
sir give the video of termination of replication in eukaryote
Pol-epsilon is attached at lagging strand while pol-dalta is attached at leading strand along with PNCA... Plz corrct this in ur video.
Sir in eukaryotic DNA replication, RNA primers are removed by which enzyme? FEN1 or DNA Pol delta
Both but mostly Fen1
little correction here, Pol delta is used for leading strand and Pol epsilon is used for lagging strand synthesis not the vise versa
Please look at leninger he is right
Very nice lecture sir.... But i have doubts dna pol require denov synthesis and u told.... Rna pol. Plz sir clear my doubts
No. Rna Pol can do de no o synthesis
Sir I have questioned that can telomeres is seen on leading strand ?because there is also 5'-3' and after removal of primer there is no 3' hydroxyl group.right or wrong sir?
?
@@devanggondaleeya4321 That's a very good question! I just drew out a few diagrams to explain everything and I think you are right. You do get telomeres at the lagging and leading strand. On the leading strand, you would get the telomeres at the 5' end on the lagging strand, you would also get the telomeres at the 5' end.
Sir..which enzyme break the RNA primer in leading strand?
The same fen1 which is used to remove rna primer from lagging strand...
Sryyy sir but I couldn't understand what is the need of looping in eukaryotes if there r two different molecules for polymerization also they r not starting from the points as it was needed in prokaryotes as there was single polymerase molecule for polymerization.also in the lagging strand u told it works in back stitching manner what we hv learnt in previous classes (11th) without looping.
But in some books there is mentioned that DNA polymerase Epsilon is PCNA independent ..Please sir help me in clearing this confusion...
It's a mystery
i watched 3,4 times even then didn't got that you are saying that it will moves from 5' to 3' but the direction acoording to fig is then wrong
+nadia khan there I mean 5 to 3 prime of the newly synthesized strand of the DNA. Let me know if the picture is not going with this message
Fin 1 exonuclease enzyme hai ya endonuclease??
Nirmal Singh endonuclease
Thnku so much sir
You're welcome
What is theta mode of replication
hello sir it use full for me thanks
sir i have dout when ever fon1 enzyme is bind the RNA primer and cleave total RNA primer but why not DNA cleavage
Super sir
You're welcome
Sir u r superb. Bt at one place u told that DNA delta when reach at Rna primer it falls off and then again go back ...which makes clear that it terminate nd do not polym.. further at rna primer...after that u told abt DNA epsilon on leading strand... then again u say that DNA delta starts cleaving RNA primer when it reach there... so this point is confusing...
New version of eukaryotic dna replication is coming soon.
sir could u pl clarify upstream n downstream concepts in brief?
Sir, you have not upload termination part of DNA relpication in Eukaryotes. Plz upload. your explanation is very nice and simple. Thanks Sir. Plz upload Termination part.
+Jami Nyitan yes. I get that request quite often. I will do that soon
Thank u sir