A line is drawn with a pencil so all samples start at the same place. One dot of the actual sample is deposited on the line. As the solvent travels up the plate the sample moves with the solvent. If there is more than one component in the sample it will separate into separate dots (sometimes the dots are elongated).
@@chemicalthinkinglaboratory735 Thank you for your answer. But my question is "the sample is applied as a band rather than as a spot". What do you think about it? And how to apply sample as a band?
They said that "the sample is applied as a band rather than as a spot" in this boos www.amazon.com/High-Performance-Thin-Layer-Chromatography-Analysis-Medicinal/dp/1588904091
That is HPTLC and this video is for TLC. High performance versus non high performance thin layer chromatography. They are 2 different techniques. Excellent question. HPTLC is performed using a lot of very expensive instruments. You can find youtube videos if you are interested.
Could you explain this to me? What is better, put a dot sample or a line sample on a thin layer? Thank you
A line is drawn with a pencil so all samples start at the same place. One dot of the actual sample is deposited on the line. As the solvent travels up the plate the sample moves with the solvent. If there is more than one component in the sample it will separate into separate dots (sometimes the dots are elongated).
@@chemicalthinkinglaboratory735 Thank you for your answer. But my question is "the
sample is applied as a band rather than as a spot". What do you think about it? And how to apply sample as a band?
@@ThuPham-tv3kt The sample is cannot be applied as a band. Only as a spot.
They said that "the sample is applied as a band rather than as a spot" in this boos
www.amazon.com/High-Performance-Thin-Layer-Chromatography-Analysis-Medicinal/dp/1588904091
That is HPTLC and this video is for TLC. High performance versus non high performance thin layer chromatography. They are 2 different techniques. Excellent question. HPTLC is performed using a lot of very expensive instruments. You can find youtube videos if you are interested.