Measuring + Isolating Fluorescence from a Combined Brightfield + Fluorescence image in ImageJ / FIJI

A viewer contacted me about a situation that seemed a bit different from doing color intensity measurement on a typical color image in ImageJ/FIJI. If you have a combined image where brightfield (or phase contrast or DIC) and a fluorescence channel are merged together, and if you do not have the separate original images/channels, you can do one of two approaches shown in this video to specifically measure the intensity of a selected area's fluorescence. Although the second method is more complex, you will be able to entirely separate the brightfield image from the fluorescence image. This is important as the white & grays from the brightfield image will still contribute green values to any measurements - and thus any green value measurements would not solely be from the fluorescence.

[@Vanessa12254]

Hello
how are you
sorry to bother you I am writing to you to kindly ask if you can give me your email to write to you about a question with imageJ that I have.
Thank you!

[@kruneuro]

Hi there! Sorry to make this indirect, but I avoid posting contact links on youtube in case bots auto-harvest links for spamming purposes. However, if you do a websearch for Kevin Urstadt Occidental, you'll find my profile page with my email.

[@Vanessa12254]

Don’t worry
Completely understable
Thank you so much!!

[@kruneuro]

Hi again - it occurred to me that I can give more info via a website, so I set a small one up. You can find my email and other resources there. sites.google.com/view/kru-neuro-lab/home