FIJI (ImageJ): Background Correction of Fluorescence Images

แชร์
ฝัง
  • เผยแพร่เมื่อ 7 พ.ย. 2024

ความคิดเห็น • 20

  • @Patrick-bv7jp
    @Patrick-bv7jp 24 วันที่ผ่านมา

    very helpful tutorial!

  • @mathewduguay2898
    @mathewduguay2898 ปีที่แล้ว +1

    Hello! Can you do any kind of quantitative analysis after applying these methods, as long as the methods are applied uniformly over all samples?

    • @johanna.m.dela-cruz
      @johanna.m.dela-cruz  ปีที่แล้ว +2

      Hi Matthew. When quantification is required, background correction is good.

  • @yanzheliu9092
    @yanzheliu9092 23 วันที่ผ่านมา

    Many thanks!!!!!💯

  • @tomaszuspienski4833
    @tomaszuspienski4833 2 ปีที่แล้ว

    Hi very comprehensive video, short and clear. Is an image processed in this way suitable for fluorescent measurement and for comparison with another image processed in the same way?

    • @johanna.m.dela-cruz
      @johanna.m.dela-cruz  2 ปีที่แล้ว

      Hi @Tomasz. If images are acquired the same way (same acquisition parameters), post-processing prior to measurements are best done in a similar way. Thanks for taking the time to give me your feedback.

  • @felinetropical8822
    @felinetropical8822 ปีที่แล้ว

    Can I perform deconvolution to an image if I had processed it with "background subtraction" or vice versa?

    • @johanna.m.dela-cruz
      @johanna.m.dela-cruz  ปีที่แล้ว +1

      Hi Feline. Deconvolution works best if images have high signal to noise ratio. Background subtraction does help to achieve this.

  • @asmaelaouina7047
    @asmaelaouina7047 ปีที่แล้ว +1

    Very informative video. Many thanks for your efforts!

  • @johanna.m.dela-cruz
    @johanna.m.dela-cruz  3 ปีที่แล้ว +2

    Remember, you can always do better!

  • @mathewduguay2898
    @mathewduguay2898 2 ปีที่แล้ว

    Great video! Are "background correction" and "background subtraction" synonymous? I always thought we needed those special coloured slides to image and work from.

    • @johanna.m.dela-cruz
      @johanna.m.dela-cruz  2 ปีที่แล้ว +1

      Hi @Mathew Duguay. Thanks for watching. Background correction and subtraction are used synonymously. They both mean correcting image defects mainly caused by uneven illumination in fluorescence microscopy post-acquisition. Fluorescent plastic slides are generally used during the acquisition process as a quick check on the uniformity of illumination and whether it is centered.

    • @mathewduguay2898
      @mathewduguay2898 2 ปีที่แล้ว

      @@johanna.m.dela-cruz Oh I see. So the coloured slides are not imaged or used in processing. That's part of what I was confused about, I have these coloured slides but no procedure for correction or subtraction mentioned them. Thanks for clearing that up :)

  • @huanyili9019
    @huanyili9019 2 ปีที่แล้ว

    Hey, I like your video very much. How can I find some datasets to practice ?

    • @johanna.m.dela-cruz
      @johanna.m.dela-cruz  2 ปีที่แล้ว

      Hi HuanYi Li. Fiji/ImageJ has sample images (File>Open Samples) that you can use for practice. You can also check out other resources at www.cellimagelibrary.org/home.

  • @cristhiancampob
    @cristhiancampob 2 ปีที่แล้ว

    Hii
    Omg please can u help me🥹 i cand do it. Even i cant download the ImageJ in the Mac. This week i have a presentation, please can u help me? Regards from Mexico

    • @johanna.m.dela-cruz
      @johanna.m.dela-cruz  2 ปีที่แล้ว

      Hi Cristhian. Send me an email. I’ll help you. By the way, you can download Fiji from here - fiji.sc/#. There’s a version for Mac.

    • @cristhiancampob
      @cristhiancampob 2 ปีที่แล้ว

      @@johanna.m.dela-cruz Wow. In first place Thank u very much for answer me. Wow.
      2. Please give me ur mail account

    • @johanna.m.dela-cruz
      @johanna.m.dela-cruz  2 ปีที่แล้ว

      @@cristhiancampob Check my “About” info in my channel.