Western blot | Western blotting protocol | Application of Western Blot | Limitations of Western blot
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- เผยแพร่เมื่อ 27 พ.ค. 2024
- This video describes the concepts behind the western blot and its usage in biomedical sciences.
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Thank u so much! It was really helpful and interesting to watch!
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Great video! Straightforward and to the point!
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I personally want to thank you, you saved my biology grade.
Please share my channel link with your friends and help me to reach big audience....lots of love from india
Gotta love the super intelligent Indian guy who explains everything infinitely better than teachers
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th-cam.com/video/gCSKpNdJkZo/w-d-xo.html also like my video entry in a global video making contest to support me
Thank you very much for the video, really made everything clear about the Western blotting method
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Why do we have to have to separate proteins by weight in the first place if we are just gonna use antibodies to find the protein of interest?
Imagine the proteins aren’t separated and they are at a single band. Try to probe for them, you’ll just get a large blob of any-protein. You won’t know what protein is what. Separating proteins of interest by weight allows for the probing of specific proteins of interest, so long as the protein on one gel is not the same size as another protein of interest you would like to probe for. You would run and probe for that second protein of interest on another gel. Hope this helped, lmk if anything isn’t clear or if I said anything incorrect
@@ala.9810 yes you are correct. If the proteins are not separated at first place then the enzyme or the antibody will not be able to bind properly, so you cannot detect the protein of interest. Other proteins if not separated might inhibit the attachment of antibody. Thank you .
Thank you sir ,this video help me to understand the concept of western blot test 🙏😊
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Thank you! Your explanations are more understandable than a lot of university professors
Thanks a lot for your compliments
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very helpful, thanks arpan!
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Your vedio is very informative but while explaining things maintain a continuous flow , don't interrupt a lot....you have done a great job!!
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Good work! Thank you 🤓
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I didn't understand half of it, but I'm appreciative that I got at least some of an idea. Cheers mate.
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Good job... It actually helped to understand the basic idea.. However sound quality was definitely an issue with this video.
I agree with you...when I made this video I did not have a nice microphone. Now I am improvising on that. Please share my channel link with your friends and help me to reach big audience.
Can you do subtitles pls.
Missed how the antibody attaches to the paper and how the wash through works.
But overall, really good quality.
This is just the overview later on detailed videos would be available
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thank you so much! only video that got me to understand
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How does one sequence the variable regions of antibodies to create binding at the molecular level
Appears no one is able to answer your question,
You explained it clearly. Thank uh🙂
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Hi, nice video. well explained. Friend can you tell me what software or webpage you use to make the animations? I'd love to make such videos for my school classes, thank you.
I use adobe illustrator and procreate
Great video bro! Thank you and keep making more please
Keep on watching my playlist
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what will we do with confirming that we could extract this desired protein?
Excellent work Sir
No sir.....Only Arpan is fine......let us get rid of this unnecessary hierarchy...we are all learners
always thumbs up, even not start watching ;)
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@@animatedbiologywitharpan yeees)
Very good!
Mephistopheles please share among your friends
@@animatedbiologywitharpan sir i got all bands are negative but positive serum control band in western blot test results... What does it mean sir...
You really explained so much in such a small vid
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Very helpful video!!
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Sir,,,, your projects based on molecular biology???
Gaurav gupta molecular biology is just a toolbox that everyone use ....I am not exception as well
@@animatedbiologywitharpan yaa bt your most videos are related to molecular biology.
Great video! Please add subtitles
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heyy very nice thanku so much 👍👍👍👍👍
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Thank you for the video! you explained in 6:31 what my lecturers couldn't explain in an hour. the one question I still have is: what is the point of SDS in western blot? surely you can skip SDS because you're using Antibodies, why do you still need to separate the proteins based off molecular weight? thank you
Good question....I will answer you tomorrow morning ( it's midnight in India)
I think you have to separate the proteins because if you didn’t there would be no way to identify your protein of interest, it would just be one band. Separating them by size gives you something to compare your protein ladder to, which has a standardized banding pattern with known sizes for each band
@@animatedbiologywitharpan and your tomorrow morning never came 😂
Thank-you! Very useful! x
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hats off
Thanks a lot for explanation!
Glad to know it was useful. You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me
Great video!!!
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very well explained
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you sir ,,, are amazing
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well done boy
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Hello sir I am a bsc biotech graduate currently preparing for iit jam 2020 can you suggest some online test series which will help my rank and also some previous year question papers book's author I can't find a good one
Honestly I don’t know cause I only did self study
By the way, I am not sir .. just Arpan is fine
@@animatedbiologywitharpan thank you Arpan 😄😄😄 I just wanted to improve my rank so that I can pursue my studies in IISC Bangalore
Ya if you want we can talk further about it
Yaa sure 😊😊😊
Hi I'm from India...... And I desperately want to order western blot... But the Drs here are not educated Enough about hsv Western blot.... I was diagnosed on a basis of a lip Infection I had and , I'm still negative on the blood test I want confirmation...is there a way I can order it in India from the University of Washington
I have no clue
thank u so much
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what is actually housekeeping protein in this context?
Joshi Joe generally people use beta actin
sorry my question was not so clear.. so what is the significance of it then,, i mean of Beta actin or housekeeping protein
great
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Kitna time lagega saara process complete hone keliye lab mei(western test for hiv)....
Atleast 6 hours
Thank u
my nigga is spitting some knowledge!!!
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Subscribed!!!!!!!!
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Thank you!
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U r da best
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Thanks! :-)
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how to extract protein from a suspected patient?
Thanks sir
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Brother please give us more information about any process
I did not get the point ....could you please elaborate
Thanks
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Grandioso video! Para qué más se usa el Western Blot, aparte de pacientes con SIDA?
Could you please write in English, I am unable to understand what are you saying
Translation: ‘’Great video! What else can you do with western blot, aside from AIDS patients?’’
Your videos are nice but just keep your accent simple and natural. That would make it more interesting.
Otherwise 👍 nice videos and explanation.
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👍🏻👍🏻💯
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Why SDS page electrophoresis is vertical ?
vertical gel electrophoresis, the gel is vertically oriented and the buffer system is discontinuous which is important for the stacking gel and running gel.this is not possible with a horizontal setup
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i love you thanks
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I don't really understand the use of this technic. Why use SDS-Page ? We could just only use the antibodies technic ?
I believe using SDS-page separates a sample into proteins based on their molecular weight (kDa). This allows for protein isolation, especially after the gel is moved to a membrane that can withstand the washing during antibody binding.
If we used just the antibody technique, they likely wouldn’t bind to anything since antibodies are highly specific to a certain sequence of DNA. A sample may contain material clumped up into various secondary, tertiary, quarternary structures with the sequence of interest covered by all the other DNA.
thank you!
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I think he missed basicly every important information about the blotting process itself.
Thanks for your feedback but its just a overview for undergraduate students and that is why all the details are not included.
@@animatedbiologywitharpan Are you working on another video for them so clever. I'm not on this level yet. But always interested to learn more. Do you know has any one in the entire world yet isolated 2019n-CoV and come up with any real images and not simply animation? I have been searching from the time it was announced, back in early 2020. However, Dr Peter Daszak reiterated that, "until an infectious disease crisis is very real present, and at an emergency threshold, it is often largely ignored. To sustain the funding base beyond the crisis, he said, we need to increase public understanding of the need for MCMs such as a pan-influenza or pan-corona virus vaccine. A key driver is the media, and the economics follow the hype. We need to use that hype to our advantage to get to the real issues. Investors will respond if they see profit at the end of process, Daszak stated.” Dr. David Martin, shared that quote, and also this one;
"The SPIKE PROTEIN in the injection is a bioweapon defined under U.S. Law
18 U.S. Code § 175 - Prohibitions with respect to biological weapons
(a) IN GENERAL. -
Whoever knowingly develops, produces, stockpiles, transfers, acquires, retains, or possesses any biological agent, toxin, or delivery system for use as a weapon, or knowingly assists a foreign state or any organization to do so, or attempts, threatens, or conspires to do the same, shall be fined under this title or imprisoned for life or any term of years, or both. There is extraterritorial Federal jurisdiction over an offense under this section committed by or against a national of the United States."
Why beta actin is use as control ??
Because beta actin levels do not change normally and it's a housekeeping gene.
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Cool, but needs better audio.
Thanks for your feedback....I would improve the audio quality eventually
Everything is good but the sound quality is very poor
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Voice not audible
So sorry for the inconvenience
Seek Christ Jesus YHVH.
Hey you said you explain biology in less than 5 minutes, but this video is 6:31. How about you stop lying?
I think 1 extra minute does not harm
@@animatedbiologywitharpan haha I was just joking
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Thanks
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