Gene expression analysis
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- เผยแพร่เมื่อ 12 ก.ย. 2024
- This molecular genetics lecture explains about gene expression analysis techniques using DNA chip technology and microarrays.
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I am a pathologist. In 3 years of residency,no faculty or book was able to explain molecular techniques in the crystal clear simple way that you do. Anyone can make a presentation super tough and intimidating. Real knowledge lies with a person who is able to come down to the simplest of the terms and explain something as alien as molecular diagnostics so easily.
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U r the only few persons in youtube who is explaining deep topic of biology at best in regular serialized manner..it helps many students...Thnx
You have got the gift of teaching sir. Not everyone has the ability.
More power to you.💚
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In 15 minutes you have helped me to understand this topic more than my university lecturer did in 1 hour. Thank you sir, sub earned
Thank you a lot. I'm psychologist and when I need to understand something about molecular biology I look for you. Congratulations! You explain very well.
I'm a pHD student from Brazil. Thank you for sharing great content, sir. I've been learning a lot w/ you.
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This is an excellent review before the midterm! Thank you very much, I had already studied the material but you made me notice some of the spots I have been missing-- I hadn't paid attention that hybridization occurred with the cDNA and the complementary cDNA strands we synthesized. It makes even more sense now. Thank you. :)
Why have I not found you before this point in my life? Thank you!
I am a data scientist trying to get my hands in biology and your videos are helping me invaluably. Thank you very much
thank you! I major in IT and about to start Bioinformatics research. This is best place to learn
This speaks about your depth in knowledge. Wonderful class
Thank you so much for appreciating my efforts
Dr. Shamus you are amazing thank you I have understood the microarray after this video. I hope you keep up doing beneficial videos especially in Plant Biotechnology field thanks
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U are awesome sir...i was struggling for this topic and not understanding at all from good books like kuby ...bt you made it so simple....
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sir .. i dont know how to thank you .. you made it very easy to understand
Shomu, you're a legend!
I'm a research scholar. My area of research is AI in Healthcare; n believe me, so far most of the concepts that I have cleared, are due to your explanation.
Thanks a lot.
:-}
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Thank you so much Shomu. You save me for my genomics courses.
Mine too😂 else i wouldn't be able to get even passing marks in genomics!
You're a legend! Keep up the good work.
Thank you so much for appreciating my efforts
One small correction: Cy3 is a red colored dye, while Cy5 is blue. When excited at a proper wavelength, Cy3 fluoresces and is seen green while Cy5 is red
Yaa dts rytt
Wow! Superb Explanation! Thank you so much and Wish you all the best!
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Such a beautiful explanation of a difficult topic like microarray analysis. Thank you for such content.
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Thank you so much. It's so helpful for an interdisciplinary student.
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Sir, I am fan of your teaching, really your teaching is wonderful, you make very complex things to be understood by even very poor person in biological sciences.
Really you are doing great service by doing such a teaching, people like us getting lot of benefits out of this.
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blue is the new YOLO XD
Amazing! how you easy explain, I was reading a scientific article. But your video saved me! know I understand it all! thank you so much!
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You help me so much for my scholarship interview
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A very good job Shomu. Gd luck
Thank you
You have lovely videos Shomu,thanks!
+Kinkini Bhaduri thank you. Glad you liked my lectures
thank you so much for your videos, I need help to understand overexpression and misexpression
Thank you so much for appreciating my efforts
@@shomusbiologyofficial can i have more explanation of over expression ?? Any reference
M a pathology pgt ... lv ur video. Thanks a lot
You're saving my life 🙂
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do you have an explanation of SAGE? Serial analysis of gene expression
my confusion have been removed Concerning to gene expression thank you my dear !
I think you may be confusing terms, gene expression correlates with levels of proteins, and not with levels of mRNA, since there are processes of sequestration or degradation of mRNAs after their synthesis and exporting from the nucleus.
I just love the content.
Thank you
Best ever explained,,, Thanks a lot
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@@shomusbiologyofficial can you explain protein level gene expression
Thank you dear Shomu. Very helpful.
Excellent job explaining it.
Thank you
In which book we get the proper note of it ..
Thanks for your amazing video,it helped me a lot,🙏🏿🙏🏿🙏🏿🙏🏿🙏🏿
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excellent sir ... u r saviour
Thank you. Glad you liked my lectures
Shomu you are awesome.
Can microarray be used to find the differential functions of a gene?
thanks shomu carry on the great job ur doing god bless u!!!!!!!!!!!!!!!
Awesome video! So one would use 3 different chips in this case? If you are using two dyes, one for the control sample and one for the three sporulation time points, would you be able to compare expression among the three sporulation times?
Please make separate video on serial analysis of gene expression
Thank u pa...😊
Use Facebook live ... you are a very good lecturer ...
+Samuel Ferrer thank you. Glad you liked my lectures
Sir! Could u pls explain about DATA MINING
is gene expression analysis is the technology behind DNA chip..???
Sir amazing explanation but you said in the yeast mRNA extraction, the cDNA is single straned. I think cDNA is double stranded please correct me....watch 8:25
I have a question. DNA chip/microarray has ssDNA embedded, however, we make cDNA from isolated RNA, as mRNA is free from introns so how this cDNA bind to its respective ssDNA present in DNA chip because the ssDNA also has intronic regions. Please explain. Thank you
Thank you for the explanation. Im mathematics student trying to understand about biological things. Anyway, when you said more control genes and less sporulation genes, how do we know what type of control genes are there (os is control genes only one type?) Also, how many percentage of each types if there is different type of control genes?
Can you explain what is protein level gene expression
not what i was looking for, but helpful nonetheless!
You explain really well! I would suggest you specialise further in biology and teaching.
Really nice top-down-thinking - never losing oversight over problem/task/exp./... at hand.
Do you have any videos that explain why measuring proteins is difficult in contrast with measuring mRNA? Is it because there are many more analytes in the case of proteins vs mRNA?
Thank you! you help me a lot. I have a question for you: after denaturation of ds DNA, we obtain two ss DNA (one that is exactly the same as the c DNA synthesized by adding reverse transcriptase to mRNA, and the other one complementary to c DNA), but if I put the two ss DNA to my DNA chip, the cDNA of my yeast expériment will hybridize only with the ssDNA that is not the same as the cDNA...so why do we put at first both ss DNA on the DNA chip? Guylaine from Canada (P.S you should teach at Harvard University)
greatly explained,,,,thanks a lot
You're welcome
Massab, it will take 1000 INR to buy a muffler-microphone or better if you get a wireless microphone and a good camera.your knowledge is very good so try to get better recording environment, even you can hire a professional on very nominal cost.
Another excellent presentation. Thank you so much!
+Deepthi Rao thank you
can you explain how dchip software works?
Excellent lecture thank you sir
You're welcome
Excellent cover topic
Thank you
I don't understand, so if you take cDNA at3 time gaps do you need to use three different chips since you are tagging all cdna red then you will now know what time point the resulting color in chip will come from.
Thank you so much sir
You're welcome
Very very nice. Thanks a lot
Discuss how you would assess whether your target gene is being translated
Can you please answer this Q🙏
you mention that c DNA is single stranded... is the cDNA is single stranded?
No, it's not!
sir .. if the cdna is not single stranded. then how it is hybridiged with its complemenary RNA in case of study in gene expresssion.. plz explain
You are confusing circular DNA with complementary DNA. cDNA is single stranded DNA which was reverse transcripted from RNA so we can sequence it. Circular DNA is what we call plasmid in bacteria, it's a double stranded DNA that is circular in shape.
Thank you sir. I want to know about locus heterogeneity.
Very nice presentation
+TIRUPATHI RAO Padi thank you very much
Literally thankyou so much
You're welcome
Sir..whats the need of isolating mRNA..why can't we take the dna of the sample cells directly..plzzz help
Thanks guru ji
You're welcome
Sir pls explain protein microarray and its types
When we put Cdna in chip so it will bind with dna from where that dna to which it will bind comes?
very helpful, thankyou
thank you so much, that is very helpful !
Thank you.
YOU'RE AWESOME! THANK YOU!!!
+Vanessa Marroquin you're welcome
what is RNA expression???
Please make one video on DArT
this video s very useful sir..
You're welcome
Thank you 😊
You're welcome
Sir plz complete one topic in one lecture bcz it takes too much time
Many thanks
Thanks a lot.
You're welcome
thank you for the video!
Glad you liked my lectures
Most amazing videos. Has always helped me through every course. Thank you so much! :)
Very articulate.
thank you so much !
You're welcome
Plssss explain in detail cap analysis, Cage
what about differential gene expression analysis?
how we can extract RNA from a single cell of yeast?
here you are comparing control yeast with the spores of yeast so why you are taking RNA from 3 spores dont you think you should take RNA fron 1 spore as genetic constitution of control and spore is same both 2n
Please explain cap analysis with diagram plssssss
Cy3 red and Cy5 is green
According to Slater
So what is true
Thank you for SMART lecture
+Tesfay Teklehaymanot glad to hear that
Plss explain the cap analysis topic plsssdddd
sir plz eplain TED and TDD algorithms
You saved me 👏
You're welcome
Sir real time pcr, SAGE aur differential display wala bhi padhao
thank you for nice sharing