Native PAGE (PolyAcrylamide Gel Electrophoresis) & Blue Native PAGE

[@DoctorJammer]

I love that you show the actual lab setting. Gives me a better frame of reference.

[@thebumblingbiochemist]

Glad you find it helpful!

[@lohanemoreira2590]

This is the best explanatory vide I've ever seen!!!!!!!!!! THANK YOU SO MUCH!!!!

[@thebumblingbiochemist]

So glad it was helpful!

[@MarianaAguilarMorales]

Hi! I have a question... I'm doing densitometric analysis of native and sds under non reducing conditions. I'm trying to figure out the nature and ideally a percentage of the type of my protein aggregates (i.e. covalent or non covalent), so I'm thinking... If I substract the band intensity of SDS-NR - native band intensity of the same protein, is it reasonable to say that that difference is my protein that's involved in non covalent assoaication?
Thank you so much!!❤️

[@thebumblingbiochemist]

Hi - I'd be worried that the different conditions might influence the staining

[@MarianaAguilarMorales]

@@thebumblingbiochemist
oh ok great point!
Thank you so much 😊

[@mengda9161]

Great post. This is just what I need.

[@thebumblingbiochemist]

Happy I could help!

[@yazallen8878]

Awesome channel! Thank you so much.

[@thebumblingbiochemist]

Thanks so much!

[@kaydenkwah5280]

OMG you're AWESOME!!!! What a lifesaver :D

[@thebumblingbiochemist]

Thank you! So glad it was helpful

[@roidedv480]

thanks for sharing！this helps me a lot

[@thebumblingbiochemist]

Glad it helped!

[@anisheshdas362]

Thank you. Reallh helpful.

[@thebumblingbiochemist]

Glad it was helpful!

[@spectator59]

Have you tried native PAGE with proteins statically bound to nucleic acids?

[@thebumblingbiochemist]

as in an EMSA? I have content on that

[@spectator59]

@@thebumblingbiochemist Yeah, EMSA's close.