Restriction enzymes | Restriction endonuclease | How does the restriction enzymes work?
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- เผยแพร่เมื่อ 8 ก.ย. 2024
- This video describes the principles of restriction enzymes and their mechanisms.
the following questions would be answered in this video.
How does the restriction enzymes work?
What are the sources of restriction enzyme?
What is restriction enzyme and its types?
What are the types of restriction enzymes?
What are two functions of restriction enzymes?
What is a restriction enzymes in simple definition?
What do restriction enzymes do in genetic engineering?
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How does restriction enzyme scan the pallandromic sequence,what helps the chemistry of enzyme or structure ?
Really cool animations..
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Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
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How can you cut your gene of interest with a restriction enzyme? what if the restriction sequence is not flanking the gene?
Even if the restriction sites are few base pair away from the promoter it's not a problem. You need a segment that has your gene , promoter and all essential elements. Some extra DNA bases won't cause problem.
Give us presentation tutorial of this vedio
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Sir from where or which part/organelle of bacteria the restriction enzymes were formed?
It's encoded by the bacterial genome.
In the In vitro application of PNPase for polymerization, how do you control endonuclease activity?
Change the conditions: By adjusting things like temperature or pH, you might be able to make conditions better for building up DNA and worse for breaking down DNA.
Use less enzyme: If you use less PNPase, there’s less of it available to break down the DNA.
Use more DNA: If you use more of the DNA that you want to build up, it can help to shift the balance towards building up DNA and away from breaking down DNA.
Use inhibitors: These are substances that can stop the PNPase from breaking down DNA, without stopping it from building up DNA.
Change the PNPase: By making small changes to the PNPase itself, you might be able to reduce its ability to break down DNA.
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