Glad someone else thinks about the syringe like I do. I use it for everything! I dont have a flow hood, so I was trying to figure out all the ways I could lessen contamination and then it hit me, USE ONLY THE SYRINGE!
I do not have a flow hood either so I do my transfers in a gallon zip lock bag that has been sanitized. I sanitize the area I am working in and then place the agar dish and the dish containing my mycelium inside the bag and close it up. Did four three days ago and just checked them. Each one growing healthy, white and strong. So far no contam. I check them the same way since it is very difficult to see into the dish that I am using. I just wipe it down with alcohol, put it in the bag opening the bag as little as possible, zip it up and I have a sterile environment inside the bag with no air flow at all. I unscrew the lid and take a quick look in.
Thank you for sharing your knowledge with the world. I started watching your videos 4 years ago when you were operating out of your basement. The first ever video I watched was you talking about your disc fogger from House of Hydro. I am a huge fan and I learn something new every time I watch your content. I can't thank you enough. Much love and respect to you.
The only reason I'd ever give one of your videos a thumbs down is the musical montages 🤣 but that's not a very good reason. Love your stuff. Thanks so much for sharing your expertise!
Thanks for mentioning this. I don't have a hood and I have seen some of the uses you mentioned before on other videos but now I know I don't need another method/tool. I also use the little condiment cups rather than petri dishes and I can put the syringe thru the cup lid for some things rather than opening the lid. I out tape over the hole.
Hey Andrew, thanks for making this video! I'm new to mycology and wanted to know what conditions (temp, lighting, etc.) are necessary to grow a liquid culture? And what is the shelf life at those conditions?
Amazing! I have to get used to it but it is a wonderful technique. Most difficult part is not to press to hard on the syringe into the agar plate. Thanks a lot for sharing!!
love this idea and as someone without a flow hood i think not opening my LC jars to inoculate from agar is a great risk reducer. Have you noticed and/or tracked any time difference between inocuation and full colonization of the jar between this method and using an agar "chunk" ? It seems it would have some effect as you are only putting a very tiny amount of MYC into the LC.
Great video! Every time I watch I get more pumped about getting into this. Do you think you will ever do a video on the principals of making the liquid culture solution. Not your recipe obviously, but just somewhere for newbies like to me have a place to start?
great advice man! appreciate it from here in Australia, I wish i saw this yesterday, i just inoculated 6 liquid culture jars lol. will def be testing mossy's syringe-scalpel tek next ;)
Interesting, u got me when u said the serenge is under used, I've developed technique to completely get rid of the serenge, but I do like how u used it to transfer agat to lc.
Hello Mossy Creek, I just found your channel. I really like your video and I think your content is GREAT. However, I do have this suggestion. I can't really tell what machine is roaring in the background (a fan, an air handler, or a flow hood maybe) but it's kinda killing your sound. It's pretty hard to hear you over it, and over time that droning just becomes more & more distracting. The result is the viewer will just stop watching the video after a few minutes (although they may not really know why). Even using a lav mic at close proximity will not be able to completely overcome a white-noise sound source like that because it's also in the same frequency as your voice. A boom mic placed as close as possible, just out of frame is a secondary but still less desirable option to just cutting off the noise source while recording. If you absolutely cannot do that then perhaps try some EQ & audio post processing, or do a voice over or dialog replacement later. Regardless... I Subscribed to your channel anyway and I'm looking forward to seeing many of your other videos. The reason I am taking the time to make this comment is that I want to keep watching a bunch more of your videos, so I want them to keep getting better. Cheers! & Power to the Fungi 💪✊
Hi ! I have a question about the liquid culture! Can you use the spores from mushroom ,and if yes what result should I look for? I've been trying to grow mycelium from store bought mushrooms since March and still nothing 😖😩😩 I've been trying many things that I've seen online with zero results ! I'm newbie and obviously I'm doing something so wrong ! Can you help with any kind of tips. Thank you .
An untested idea- use bacillus thuringiensis (BT) in your fruiting block mixture. Is organic and approved for use on fruit and vegetable crops the day of harvest. I havent tried this nor do I know if it's a great idea but, having had gnat troubles before, I have envisioned using BT in my bulk substrate mixture to make the substrate inedible to gnats and thereby ward off the possibility of infestation. Thoughts?
You injected cultures into fresh water, but why? What will you then use that water for? Do you allow that culture to grow in the water? Does it not need any food source?
I had to watch it a second time. I thought the same exact thing. But I think he was only sucking the sterile water out, then grabbing some agar culture and injecting into new agar or sterile sugar water to make more liquid culture. I like it! @ 3:30 he gets agar culture and then injects it into a jar. That jar is not the sterile water, it's future liquid culture, which is sterile sugar water. That's what threw me off.
Glad someone else thinks about the syringe like I do. I use it for everything! I dont have a flow hood, so I was trying to figure out all the ways I could lessen contamination and then it hit me, USE ONLY THE SYRINGE!
I do not have a flow hood either so I do my transfers in a gallon zip lock bag that has been sanitized. I sanitize the area I am working in and then place the agar dish and the dish containing my mycelium inside the bag and close it up. Did four three days ago and just checked them. Each one growing healthy, white and strong. So far no contam. I check them the same way since it is very difficult to see into the dish that I am using. I just wipe it down with alcohol, put it in the bag opening the bag as little as possible, zip it up and I have a sterile environment inside the bag with no air flow at all. I unscrew the lid and take a quick look in.
Thank you for sharing your knowledge with the world. I started watching your videos 4 years ago when you were operating out of your basement. The first ever video I watched was you talking about your disc fogger from House of Hydro. I am a huge fan and I learn something new every time I watch your content. I can't thank you enough. Much love and respect to you.
Nothing but love for you my G👍🏽👁️🍄
The only reason I'd ever give one of your videos a thumbs down is the musical montages 🤣 but that's not a very good reason. Love your stuff. Thanks so much for sharing your expertise!
Haha. Such nice work! LCs to grain are my favourite to remove vectors of contamination. But Syringe is the next step up. Great. ❤️❤️
Thanks for mentioning this. I don't have a hood and I have seen some of the uses you mentioned before on other videos but now I know I don't need another method/tool. I also use the little condiment cups rather than petri dishes and I can put the syringe thru the cup lid for some things rather than opening the lid. I out tape over the hole.
syringes can be autoclaved for reusing them couple times cutting down the waste/cost
Hey Andrew, thanks for making this video! I'm new to mycology and wanted to know what conditions (temp, lighting, etc.) are necessary to grow a liquid culture? And what is the shelf life at those conditions?
Great video! I love those tips. How long and how often do you put your LC jars on the stir plate? How long till the LC is ready to inoculate grains?
Amazing! I have to get used to it but it is a wonderful technique.
Most difficult part is not to press to hard on the syringe into the agar plate. Thanks a lot for sharing!!
this is dope , keep on inform us of your work , bravo
The musique choice always great, share your playlist at work with us !
Revolutionary video .One question.. can't i directly take LC and take biopsy sample instead using sterile water??
love this idea and as someone without a flow hood i think not opening my LC jars to inoculate from agar is a great risk reducer. Have you noticed and/or tracked any time difference between inocuation and full colonization of the jar between this method and using an agar "chunk" ? It seems it would have some effect as you are only putting a very tiny amount of MYC into the LC.
Great stuff! Question for you @mossycreek - what do you recommend FFU or laminar? Where’d you get yours? Would love your insight!
Dude your music is always on point!
Wow - thanks for that hint! My life will be much easier from now on! The syringe will be my favourite tool too :-)
Awesome video! Can you tell me what song that is starting at 9:32!
This is a game changer!!! I've been having contam issues with my Agar to LC. This technique will be used next time.
WHAT A KIND AND NICE PERSONALITY
Thank you so much for this video. Great idea. I am going to try this very soon.
Andrew, great tip! I always enjoy watching your videos. P.s. thanks for the tip on the peptone in LC as well!
Great video! Every time I watch I get more pumped about getting into this. Do you think you will ever do a video on the principals of making the liquid culture solution. Not your recipe obviously, but just somewhere for newbies like to me have a place to start?
youtube peter mccoy
Andrew gave his recipe on one of the life streams. Don't remember which one off-hand...
Can use Light malt extract, or your grain water after boiling your grain.
Distilled water
Sterilize
Thank you for this wonderful tip! That's smart idea!
You are so welcome!
Do you get a new needle each time you get a new sample, as to not contaminate your H2O jar? Or do you flame sterilize between samples?
Hello Mossy, how often do you mix LC? How many minutes is ideal? Thank you.
Thank you very much! Got my wheels turning.... mush 🍄 ❤️
You’re welcome 😊
Very cool video!
Awesome video. Game Changer!
great advice man! appreciate it from here in Australia, I wish i saw this yesterday, i just inoculated 6 liquid culture jars lol. will def be testing mossy's syringe-scalpel tek next ;)
Thanks for the tips sir !! Really2 usefull
Interesting, u got me when u said the serenge is under used, I've developed technique to completely get rid of the serenge, but I do like how u used it to transfer agat to lc.
Does the syringe extract enough tissue to inoculate your grain or LC? Very cool idea!
Hello Mossy Creek,
I just found your channel. I really like your video and I think your content is GREAT.
However, I do have this suggestion. I can't really tell what machine is roaring in the background (a fan, an air handler, or a flow hood maybe) but it's kinda killing your sound. It's pretty hard to hear you over it, and over time that droning just becomes more & more distracting. The result is the viewer will just stop watching the video after a few minutes (although they may not really know why).
Even using a lav mic at close proximity will not be able to completely overcome a white-noise sound source like that because it's also in the same frequency as your voice. A boom mic placed as close as possible, just out of frame is a secondary but still less desirable option to just cutting off the noise source while recording. If you absolutely cannot do that then perhaps try some EQ & audio post processing, or do a voice over or dialog replacement later.
Regardless... I Subscribed to your channel anyway and I'm looking forward to seeing many of your other videos.
The reason I am taking the time to make this comment is that I want to keep watching a bunch more of your videos, so I want them to keep getting better.
Cheers! &
Power to the Fungi 💪✊
How about the lion's mane mini farm... Website said can't find the page!!!
Thanks for the tip
you are a genious men! greetings from argentina!
How much time you can use these lids ? how much time you can inject them ? Ho know ?
Love your info ty four videos god bless
I LOVE this! Great advice!
Do you ever do spore prints and are they a good thing to do?
Wow just wow
This is an awesome idea thank you so much
Thanks for watching!
Thank you!!!!
could you be introducing contamination from the agar plate to the water?
only if your water is dirty, which being sterilized, it shouldn't.
Hi ! I have a question about the liquid culture! Can you use the spores from mushroom ,and if yes what result should I look for? I've been trying to grow mycelium from store bought mushrooms since March and still nothing 😖😩😩 I've been trying many things that I've seen online with zero results ! I'm newbie and obviously I'm doing something so wrong ! Can you help with any kind of tips. Thank you .
Go over needle sizes
I give a try, thanks buddy from cali.
Hi sir is liquid mushroom spawn have expiration thanks sir I'm from philippines
What size syringe needles
18 gauge x 1.5 inches
@@mycominer way to be!
love all your videos bro! as we say in Uruguay, El andrew sapeee XD
What do you use to control/eradicate fungus gnats? I recently lost hundreds of fruiting bricks and cakes to a fast infestation.
An untested idea- use bacillus thuringiensis (BT) in your fruiting block mixture. Is organic and approved for use on fruit and vegetable crops the day of harvest.
I havent tried this nor do I know if it's a great idea but, having had gnat troubles before, I have envisioned using BT in my bulk substrate mixture to make the substrate inedible to gnats and thereby ward off the possibility of infestation.
Thoughts?
Upstairs for thinking downstairs for dancing
Now that's getting the most at of what you got
You injected cultures into fresh water, but why? What will you then use that water for? Do you allow that culture to grow in the water? Does it not need any food source?
I thought the same thing for a second. He's using the sterile water as a method to push out the sample taken into the end of the syringe.
I had to watch it a second time. I thought the same exact thing. But I think he was only sucking the sterile water out, then grabbing some agar culture and injecting into new agar or sterile sugar water to make more liquid culture. I like it!
@ 3:30 he gets agar culture and then injects it into a jar. That jar is not the sterile water, it's future liquid culture, which is sterile sugar water. That's what threw me off.
Now I think I know why my LC is failing too frequently
I love your channel, you guys rock. what is a the premix mixture % you use in solution?
Have you tried to inoculate grain with agarics liquid culture?
❤
🤯🤯🤯🤯
I made it controversial I love the way you brief
Blessed Corona!
I see Ben's been busy....you got your table redone.
I have in fact been quite busy
first :)