Hello, can you maybe do tutorials on how to create plasmid in say benchling? How to do them and how to put them in a backbone etc. and what to order from it to get the right plasmid for transformation. Thanks :)
So you replace the liner every 150 samples as well? What type of analysis are you running on this machine, and does the liner last longer with cleaner or more dilute samples?
Shimadzu just recommends every 100 to 150 samples. I think the liner can become more or less dirty depending on your samples. I'm running 5uL samples of dodecane and the liner is visibly dirty after 100 samples.
@@DIYBiotech i have assembled a lab, which has GCMS, GCFID, HPLC-UV, HPLC-DAD now, but ive got way more hours repairing and learning to run them than actual analysis, which i plan on doing pretty soon. Ive read a lot about liner de-activation, and how over time they can become active and affect your chromatography, have you ever noticed a decline near the end of life of a liner? I guess also, applications like MS might be more sensitive than FID of course.
@@Vanisletechstuff I run GC-TQMS on split and splitless liners, and I've used a single liner for hundreds of 1ul injections with no problems. I'm sure it really just matters on the content of your injections.
Thanks for watching! Let me know if there are any other protocols that you would want to see.
Hello, can you maybe do tutorials on how to create plasmid in say benchling? How to do them and how to put them in a backbone etc. and what to order from it to get the right plasmid for transformation. Thanks :)
So you replace the liner every 150 samples as well? What type of analysis are you running on this machine, and does the liner last longer with cleaner or more dilute samples?
Shimadzu just recommends every 100 to 150 samples. I think the liner can become more or less dirty depending on your samples. I'm running 5uL samples of dodecane and the liner is visibly dirty after 100 samples.
@@DIYBiotech i have assembled a lab, which has GCMS, GCFID, HPLC-UV, HPLC-DAD now, but ive got way more hours repairing and learning to run them than actual analysis, which i plan on doing pretty soon.
Ive read a lot about liner de-activation, and how over time they can become active and affect your chromatography, have you ever noticed a decline near the end of life of a liner?
I guess also, applications like MS might be more sensitive than FID of course.
@@Vanisletechstuff I run GC-TQMS on split and splitless liners, and I've used a single liner for hundreds of 1ul injections with no problems. I'm sure it really just matters on the content of your injections.