How to read STR electropherogram

An electropherogram (or electrophorogram) is a record or chart produced when electrophoresis is used in an analytical technique, primarily in the fields of molecular biology or biochemistry.
In the field of genetics, an electropherogram is a plot of DNA sequencing results generated by Sanger sequencing. Such plots are often achieved using an instrument such as an automated DNA sequencer. Such electropherograms may be used to determine DNA sequence genotypes, or genotypes that are based on the length of specific DNA fragments. These genotypes can be used for:
genealogical DNA testing
paternity testing
genetic fingerprinting
phylogenetics
population genetics

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Do you have the video about how to calculate allele frequency in the population?

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Very useful thank you very much… Do you know what does the area under each peak refers to???

[@andrew-hv1hv]

it could refer to the relative abundance of the analyte in the sample, so if theres a large area then theres more of it, and so on

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How do you detect 2-5 bp deletion/insertion

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By designing primers, and PCR of the fragment that include deletion, with following sequencing.

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Do you have any videos which can show deletion pattern for a particular STR marker..?

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Wait so what do the peaks of the electropherogram represent?

[@GeneticsLessons]

Presence of allele.

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i have a question how will look like trisomy 21 on this chart ?

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after getting the data how to know the phenotype of the data

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