Nice job! but quick question I am Ph.D. student in the pharmacology and toxicology department with a pharmacy degree background wanted to know how feasible it is to learn transcriptomes in 6 months internship?
When you do multiple rounds of smFISH, are you moving the slide from the microscope to the bench and back again after each round? How are you able to preserve the same position when you overlay the multiple images? A small shift of the slide placement could result in a shift image, right? How is this overcome?
There is no need to remove the slide every round. In the present comercial platform using MERFISH, the slide is set in a flow chamber, and the different probes will fluid in automatically after the former round's imaging. Also the objective lens will also automatically move to the next FOV.
@@xiaohuiyu1928 It's lovely to see that you came back and answered your own question (I mean that genuinely). I am aware that throughput usually refers to the number of cells that the technique can handle; in scRNA-Seq, my area of focus, droplet-based methods are very high throughput but they lack sensitivity whereas plate-based methods are exactly the opposite (high sensitivity and low throughput). What would multiplexing mean in this context though? I would appreciate your input.
Great workshop! Thanks a lot!
讲的真好！帮助了我很多，非常感谢～
Brilliant. Thanks a lot
Great work! Thank you!
Nice job! but quick question I am Ph.D. student in the pharmacology and toxicology department with a pharmacy degree background wanted to know how feasible it is to learn transcriptomes in 6 months internship?
Great! One observation, we cant do double, triplo fish, Fish and immunofluorescence etc.
Could you provide more detailed info regarding the publication @31:21? Michael Nunn, 2020. I can't find the orginal article.
When you do multiple rounds of smFISH, are you moving the slide from the microscope to the bench and back again after each round? How are you able to preserve the same position when you overlay the multiple images? A small shift of the slide placement could result in a shift image, right? How is this overcome?
There is no need to remove the slide every round. In the present comercial platform using MERFISH, the slide is set in a flow chamber, and the different probes will fluid in automatically after the former round's imaging. Also the objective lens will also automatically move to the next FOV.
what's the difference between multiplexing vs throughput?
Multiple rounds vs number each round.
th-cam.com/video/URqjNcZ7d5E/w-d-xo.html
@@xiaohuiyu1928 It's lovely to see that you came back and answered your own question (I mean that genuinely). I am aware that throughput usually refers to the number of cells that the technique can handle; in scRNA-Seq, my area of focus, droplet-based methods are very high throughput but they lack sensitivity whereas plate-based methods are exactly the opposite (high sensitivity and low throughput).
What would multiplexing mean in this context though? I would appreciate your input.
can i have the data mentioned in the video for processing?
thank you!
20:00
31:00
can i have the data mentioned in the video for processing?