How to Make Psilocybin with Yeast | Journal Club
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- เผยแพร่เมื่อ 8 ต.ค. 2024
- Psilocybin and psilocin are the active ingredients in magic mushrooms. In this paper review, I go over how researchers genetically engineered baker's yeast to produce these valuable compounds! The paper is titled:
Metabolic engineering of Saccharomyces cerevisiae for the de novo production of psilocybin and related tryptamine derivatives
www.sciencedir...
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Your channel is litterally the best one in the biotech category tbh
Thanks!
@@DIYBiotechI was just thinking about doing this, but for salvia. The diviner sage I believe it's called salvanorium a
Kegg of psilocybin got me tryptophaning.
What a time to be alive!!
I think you're right about the future where you can brew your own psilocybin in a jar, and I think that's pretty dope :D
That's how you grow mushrooms
For recreational use, it's already easy to legally get spores online and get to growing. This kind of stuff is more for mass production to enable low cost use in therapeutic settings.
Erm "in the future" ?? Anyone can do it now, just order the mycelium and a bag of grains as a kit, even a kid can grow these.
@@orion9k Well to get psilocybin you don't even have to grow anything yourself. Just go to your local park and look for naturally growing Psilocybe-species, like P. semilanceata. They are pretty widespread and contain high concentrations of those active compounds. Just remember to not eat any mushrooms that you're not 100 % sure what it is.
So yeah, getting psilocybin is not a hard task and anyone could do it.
But there is a big difference between growing your own mushrooms from mycelium and grains and putting some yeast in a bucket with water and sugar and getting psilocybin out of it. And last time I checked there wasn't any vendors offering these kinds of yeasts for sale, so brewing your own psilocybin is still a thing of the future.
@@theKashConnoisseur yeah that is the intended use for this tech, but when it escapes the lab to the streets and the ability to mass produce psilocybin is in anyone's grasp we can finally say that the drugs have won the war on drugs.
Commenting before this goes viral, already sent it to my mushroom buddies. 😂 great video
Legend!
This is the best news I've seen in a looooong time!!!! ❤😊
Taught me more about biotech than any others, don’t usually comment but this is a really great channel, I would pay for a course!
Much appreciated!
so what you're saying is: "I want large quantities of psilocybin" 😂
we can design and release a highly competitive yeast strain that rapidly replaces all normal yeast in the world, and produces psilocin, so now bread gets you high, and bread will be like that forever basically.
Muhahaha
Most bakers use a single strain of yeast for each type of bread. The same scrutiny is exercised for beer and wine as well as mashes for spirits. There are other situations where it would slip on there. Sourdough bread is usually a local yeast bouquet from putting a dish of water with a touch of flour out, then when the tase is preferable, the "mother" batch is kept and regrown in a dish with a cloth over it to keep other spores out. Occasionally, they will split the mother and allow more local yeast to settle in. In that case, you could definitely affect a sandwich. Some fruits are fermented with local yeast as well to produce wines. If you have ever seen the video of the chateau elan reporter (ooh oooh ooh ooooh) making wine, you see her smash grapes with her feet. Think about that, your feet generally stay warm, dark, and often moist, making a perfect habitat for yeast to live. There are other places on the body susceptible to yeast reproduction. Think infections specifically in the downstairs regions. Now imagine a foot colony or other colony on your body producing a constant supply.
Now imagine how long it would take the general public to figure out they aren't just tripping over their feet, but tripping because of them.
Crazy
it probably doesn't get you high any more once your brain adjusts.
psilocybin breaks down at high temperatures
I have never clicked on a video faster
It’s all fun and games until you get a yeast colony in your gut and end up tripping 24/7
YO
@@DIYBiotech❤😂👍
nah unless you intentionally try to do it, giving yourself auto-brewery syndrome is pretty hard. The body becomes tolerant to this drug insanely fast too so I can't imagine the effects would really last all that long even if you did succeed
The transition to that being your base reality is wild to think about, its like when you're tripping, and you feel like you'll be tripping forever, except you actually do in this case...
this will be your biggest video. wait and watch xD
You gonna get some likes with this one lol
Dude no joke he just summoned a whole neighborhood of communitys 😂😂
Valid comment
Tru
Already got recommended to me and I have no search history in any of that stuff lol
W algorithm pull.
Right!?
Magic Beer would fly off the shelves in stores! One beer per gram would be perfect.
😂you taking some weak shrooms, or you drink like a Muslim😂one of the two, cause a beer and a gram of shrooms are not equivalent. Put like 0.3 in each
Awesome video
I had theorized that this was possible along with THC and CBD pathways in alge, but thank you for fleshing this vague idea out. Great stuff!
THC/CBD video may be coming soon...
@@DIYBiotechwow really? So honestly could you tell me how viable this would be for a home lab?
@@microfxyou might wanna look into conversion ofc cbd into thc, which is really easy imo
@@DIYBiotechthey already do cbd and THC. I forget the name but one of the companies that does it is out of Québec.
Yup it does work as algae have the right hardware for efficient biosynthesis of lipids.
holy fuck brother i have been into this subject for years and you just blew my mind,. thank you for your dedicated work, kudos to you
This paper got me into biotech
woow, this knowledge is applicable for so many biochemical synthesis, thank you so much for sharing!
Very very nice video, it's not easy to learn BioTech (or not as easy as many other topics) by yourself and you made a amazing job explaining this paper. Had a little ChatGPT window to get some definitions or insights on things you mention I didn't know or remembered.
Also 100g equivalent of dry mushrooms in a 1L bioreactor is insane !
Glad it was helpful!
BTW - a bright red dragon told me getting the dose right is a bit tricky. A lot of plants produce DMT so maybe those pathways are known and that molecule will be next. As a Chemical Engr. I see DMT as better suited to extraction, purification, analysis, quantification and storage for the home craft brewers. Now you've got me thinking about how to process the Psi-brew. Thanks for this video, and congrats on your PhD.
isn't psilocybin already pretty close? 4-HO...
@@microfx Structurally yes. Purification, isolation & preservation require higher skills. DMT processing is a known art. Having said that it will be interesting if the yeast method gets out in gen pop and producers find a product form that meets consumer requirements. I'm betting the community works out those challenges.
Can you isolate dmt from animals more easily? I'm not interested in DMT as a drug I just am interested in the chemistry and stuff.
The idea is really crazy. It is amazing they figured this out. The yeast is literally just eating sugar and making the end product?
yea exactly! Imagine if people had this in their kitchens...
Woud be better to supplement with tryptophan and ammonium dihydrogen phosphate and other stuff to push the equation more in favor of psilocybin synthesis.
Mind, officially, blown 🤯. This is game-changing. Im going to have a hard time sleeping tonight for sure. A lot to think about 😁.... Hahaha yes you did trick me with drugs to learn about metabolic engineering and yes it was interesting for sure😂 great video!
THC in yeast video tomorrow ;)
highly fascinating. thank you for sharing!
Glad you enjoyed it!
Magic beer.
Mmmmmm, beer.....oh....oh shit
Imagine if you got autobrewery syndrome with that yeast 😮😮😮
This yeast culture would become popular, for home use.
There's a paper from around 15-20 years ago on making hydromorphone from yeast that I've been waiting to see actual usage. A video on that though similar would be interesting too. The paper was widely reported at the time but I haven't heard anything since.
opioid production in microbes has had a big push recently, but as far as I know, titers are still in the ug/L range, which may make the process not very economically viable yet. I did find some older papers on opioids and microbes, one where they fed a bacteria morphine as the sole carbon source? If you have the specific paper you want me to look at, shoot me a message or an email!
@@DIYBiotech I'll see if I can find it, I think they were starting with sugar.
Nice video to ho with a few others out there. Opioides grown in yeast. Then this. I expect to see some soon on THC in yeast and coca and other social drugs in yeasts. From biofactories to genetically engineering enable plants and fruits and such. Thank you for sharing the video. A good chunk of chemistry in them all.
THC in yeast video tomorrow ;)
idk about psilocybin - but I'm trippin on how much research and just how the heck did they do it in order to produce the knowledge to build those data maps.
Imagine being able to do this for certain central nervous system stimulants, looking forward to that haha
imagine shaking a jar and then harvest enough material for a month quickly
@@microfx😂😂😂
Walter White has entered the char
Brewers yeast will reduce tryptaphan to tryptaphol as it is, then put an enzyme coded to attach a dimethylamine tail on it. At that point you got dimethyl tryptamine. For psilocybin you would want the enzymes from the fungi and increase the free phasphate added as diammonium phosphate to push the equilibrium toward the production of psilocybin.
"This paper might be more helpful if you actually want to do this project."
> *Proceeds to chug from container that looks suspiciously like 1L in volume.* 😳
can’t wait to take a 4 year biochem degree to spend 4 months genetically engineering the thing that makes bread rise to see shadows laugh at me
ho ho let the search for some special yeast begin.. guess its gonna take some time before the man catch up to the magik brewers yeast hehe
“I’m comin’ for every enzyme” 🧛♂️
The should bake some psychedelic bread
I haven't watch the video yet but the title makes me imagine somebody buying some beers thinking their just gonna get drunk but accidently ingest Psilocybin lol
sassy pic goes hard brother
@@sopeylive Thanks I drew it a few years ago
someone should totally reproduce this result. for science of course
Yeah and make a tutorial for beginners how to do it at home.
@@lijuminati We're Breaking Bad with this one!
@@soumyobiswaslko or baking bread ...depending on the yeast and other ingredients 🤪
makes me wish i had finished my chemistry degree program sooo many years ago ~
Can you please make a DIY tutorial for making galactosyl-lactose (aka beta-GOS) at home? Evidently certain kinds of beta-galactosidase enzymes under certain conditions can cause partial hydrolysis of lactose and also combining of galactose with lactose to form galactosyl-lactose. Beta-GOS is the best prebiotic in the market and the literature for improving lactose tolerance.
I'll look into this! Very new to me.
Is casomorphone addiction the reason people that are lactose intolerant won't give up on dairy? Similarly, if I had celiac, I wouldn't eat wheat, not look for more ways to manipulate it so I could eat something harmful, as a drug. Guess that's just me being too logical again, sigh.
If a "food" made me sh*t my brains out, I probably would take the hint and avoid it, but that's me.
Pouring sugar over the mucky areas, will update on progress
i got a general question on such engineered yeast strains: do they diverge from the engineered variant after a while? is there a contamination risk with wild yeast strains etc.? i have no idea about any genetic engineering/biotech topics, i just find it fascinating and also saw some simpler DIY stuff about it on the thought emporium which was interesting to see how it works in practice. i'm wondering because i've heard about these types of yeasts quite often but i'm wondering if it could ever happen that someone reproduces one of these papers and just starts selling this stuff 🤣or if that's unreasonable because it'll degrade too quickly
People could definitely make this and sell it! Yes, over time the genetics of the strain will change. It is not evolutionarily advantageous to have these bulky, irrelevant genes integrated, so the yeast will eventually lose them given enough generations. However, we can create glycerol stocks (there's a video on this on my channel!) of the yeast to basically freeze them in time. It's like finding an heirloom tomato seed from a 200 year old tomato or something. The genetics will be very similar to the first sell that had those genetics.
Can it work on other plants medicine 😅 the next wave of brew speed lol 😅
Yes! I got more videos on this coming!
THIS is alchemy.
Bookmarking this channel to come back for a status update on his psilocybin synthesis journey and recruitment of willing beta testers 🙏 aka underground human trials..🤔
Alright alright you don't have to twist my arm, ok I'll do it. I'll pioneer the front genetically modified mutant mushroom sludge
STFU it's my fantasy and I'm allowed to have it
That's nice and all but how do you purify it? You're going to have to separate out the product from yeast culture.
Do you purify with a large column? What kind of column do you use to purify it too? I would be interested in seeing a paper purify a small molecule product like that from cell culture.
Can you use an instrumental separation technique to purify it from cell culture in fraction pooling?
Like these are the things I'm curious about lol.
Yes this is definitely a concern! But there are plenty of extraction protocols out there, here's one: www.researchgate.net/profile/John-Casale/publication/19122936_An_Aqueous-Organic_Extraction_Method_for_the_Isolation_and_Identification_of_Psilocin_from_Hallucinogenic_Mushrooms/links/5a56465ba6fdcc30f86d4df3/An-Aqueous-Organic-Extraction-Method-for-the-Isolation-and-Identification-of-Psilocin-from-Hallucinogenic-Mushrooms.pdf
18:50 so realistically you are saying it will take you at least 5 months to make that sketchy website.
cool but its fairly easy to grow magic mushies from spore at home. rather see yeast that can produce things that have to be synthesized usually... 2cb, mdma, ghb, k, etc. is that even possible?
For some things it is possible! Just gotta find the right enzymes to catalyze each step.
thanks my boi - just ship me the psychedelic yali i saw the rest of your channel, i know you be holding.
If I had it, you'd know!!
@@DIYBiotech word tho - great work and you lit a fire up under my ass - lol growing whole fruiting bodies - so last century
Subscribed!!😎👍
Amazing chanel
Makes sense that they did it more easily in another saprophyte. I wonder if it opens the door for production in other unrelated saprophytic fungi, though brewers yeast has to be one of the most convenient.
Yep! Brewer's yeast is the most well studied yeast and thus the easiest to work with (also very high homologous recombination efficiency). I'll discuss a bit in my next videos about non-conventional yeast, but with recent advances in genetic engineering, other organisms are much easier to work with now.
@@DIYBiotech Yeah that high HR efficiency must make it an easy choice for this kind of work. Always nice when something like that appears in nature. Kinda like how P. Cubensis has the good fortune of being so easy to cultivate on so many different substrates. Little serendipities, I guess!
Gotta say though, in dissent with your opinion on the inclusion of Methods and Materials sections, I rather like seeing them, not just for more intensive peer review and replication, but for transparency and the democratization of science as well. Certainly a specialist might be able to infer the process from the rest of the paper or even contact the authors, but I like the idea of non-specialists being able to either replicate the results or concoct offshoot experiments based on the specific methodologies employed. I mean, we wouldn’t be able to have the “will someone try this at home?” conversation without it. Moving away from them feels like a “pay no attention to the man behind the curtain” moment, and even with the best of intentions still might come across that way. I’d think, especially given the current academic climate, we’d want to reduce that kind of academic stratification at any opportunity, even if the inclusion of that information may be redundant for certain parties. I’m interested in what your rationale is with regard to not liking them. Maybe I’m missing something?
But can it still ferment alcohol?
The strain from the paper probably makes some alcohol, but likely less than 1%. You could start with a strain that makes a lot of alcohol and modify from there instead! Not all S. cerevisiae make a bunch of alcohol.
Make a video step by step and explain everything that would be great. Thank you.
Note to self: buy yeast in bulk.
more like yeast and 50 other ridiculously expensive compounds. Only yeast and the only thing you'll achieve is a fart.
@@LoraB-e5lwill the fart contain psilocin?
Making this is keggs right now!!
about time
………. Alchemy at its finest^^
✨༺❀🍄⚜️x🤘🏻x⚜️🍄❀༻✨
sooooo where do I get this yeast?
You gotta make it
Let's get the LSA pathway to make LSD 😊
I believe this had been done already! Maybe I'll make a video on this soon!
@@DIYBiotech no way 🙃😆 that would be very interesting. I just ran across this channel, but I have read about many similar efforts utilizing yeast including the production of ephedrine and some opiate precursors. It is a very interesting topic. Thank you.
dmt from yeast before gta 6
It's already relatively easy to extract.
Why would you not want materials & methods in the study? How would peer review work? How would you check that their experiment wasn't poopoo?
Great question! When we peer review a paper, we don't actually recreate any experiments, typically. We just take their word for it. I wasn't super clear here, but the only time we use materials and methods is if there is a small part of the experiment we want to copy for our own papers we are working on. It is more common that people want to just read the results. We usually don't have time or don't care about the minute details on how they got their data!
Thanks
If you only feed glucose.. what is the source of nitrogen in the end product?
So looking at this study we should only be a few years from the synthesis of psilocybin/psilocyn? I am a high school dropout, so some of the terms went over my head of course. Very inspiring if true. Great presentation by the way very easy to understand.
We are already here! If anyone from that lab wanted, they could steal a sample of the yeast and give it to anyone to grow
@DIYBiotech that's very good news, especially with growing mental health crisis right now
Since AI has figured out how to fold proteins and such, maybe it can figure out how to make this process easier?
There's a big push for automation in the industry, but in the end, you gotta have someone or something to interact with the physical world!
Now if you could do that with 4ACO then you're onto something :D
Could definitely do it chemically! But maybe there's an enzyme out there that could make this... Or maybe use a different precursor and hope the enzymes in the pathway are promiscuous to it.
NGL i called this like 30 years ago
It's funny how few Chinese names there are on that paper.
5 pounds you say 🤔🤯🍄
Assuming you don't already have a tolerance...
Shrrrooooommmmssss!!!!
🍄💨
thats soo crazy...
We getting bread cancelled with this one
I know they did this for morphine already
Might do a video on that soon!
Can we put it in vaginal yeast so woman can know they have an infection when the walls start melting?
Do you mind if I call you my fliend?
Thank you my fliend
Eating a pound of dry mushrooms might not kill. But you sure would feel like it. 5 grams is enough for you to see God
Casually plucking mushrooms from my nose and ears till the day I die. 😂
Who's helping the homeless? Where can they be safe?
In mars, duuuuude
Do not use this type of Psilocybin.
NEVER
explain
This guy doesn’t know
Sorta related to this, it's probably just fiction but I just started watching the new Penguin TV show & they supposedly developed a "new drug" and it surprised me when they showed a chemist, mycologist who found a way to grow bleeding toothed fungi that ooze a red fluid drops 🩸 🍄 and they extract a psychoactive drug from that red fluid that's different than the usual 2 compounds you usually get from psychoactive fungi. I know it's probably just made up movie BS but it still interested me because I know those bleeding tooth fungi are real. I wonder if anything can be utilized thru that?
This could realistically be done in the next decade. Not the most practical way to make chemicals, but it could be done with relative ease.