How to use Nanodrop to measure DNA/RNA

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  • เผยแพร่เมื่อ 30 ม.ค. 2025

ความคิดเห็น • 20

  • @JaceTan-90
    @JaceTan-90 5 หลายเดือนก่อน +1

    Simple, straightforward and clear cut! Thank you!

  • @kamaljitlall9329
    @kamaljitlall9329 ปีที่แล้ว +2

    Absolutely marvellous video. Very concise, stepwise, explanation. Thank you.

  • @MarceloSMota-vi8zd
    @MarceloSMota-vi8zd 5 หลายเดือนก่อน +2

    Very useful video! Thank you!

  • @sanjit666
    @sanjit666 2 ปีที่แล้ว +1

    Nice crisp demo

  • @HamdiAL-AZZANI
    @HamdiAL-AZZANI 2 ปีที่แล้ว +1

    Thank you so much dear brother

  • @sara97979
    @sara97979 2 ปีที่แล้ว +1

    Great explanation, thank you 🙏🏻

  • @parvizabdullah
    @parvizabdullah 3 ปีที่แล้ว

    Thanks a lot for your explain. It was very useful for me.Hope you'll share new videos.

    • @innovative_learner
      @innovative_learner  3 ปีที่แล้ว

      You are welcome. Its a pleasure for me. Please let me know what kind of videos would you like me to post. Thanks.

  • @sufiyan5095
    @sufiyan5095 3 ปีที่แล้ว +1

    Thanks a lot for your explanation it's so useful Sir
    I have a doubt
    3.29 what is that 50 below sample ID and beside DNA
    THANK YOU SIR

  • @mohi7527
    @mohi7527 2 ปีที่แล้ว

    Hi, thanks for the explanation. Did you add Milli-Q water or DE water for cleaning the lens?

  • @hanyabdelgawadabdelsalamab7470
    @hanyabdelgawadabdelsalamab7470 2 ปีที่แล้ว

    Nice video, thanks for your help. Since you loaded 1.5 µl, is the result of the reading for 1.5 or for 1 µl ? ie the machine knows you loaded 1.5 and does the calculation automatically ?

    • @innovative_learner
      @innovative_learner  2 ปีที่แล้ว +1

      It's not about the volume actually. The liquid fills the gap between the two surfaces. Moreover the volume used was consistently same. No, you don't need to enter volume in the programme.

  • @jesse4828
    @jesse4828 2 ปีที่แล้ว

    how do you get the raw data off the computer. all I can get is the report

  • @정성훈-n9r
    @정성훈-n9r 3 ปีที่แล้ว

    What are the principles of nanodrop machines?
    I know you're using the uv-vis method.
    How do you measure the dna concentration and wavelength?

  • @SuleimanNasiru-ot3jy
    @SuleimanNasiru-ot3jy 2 ปีที่แล้ว

    How can I use the values gotten to calculate the purity

    • @innovative_learner
      @innovative_learner  2 ปีที่แล้ว

      Please consult the following link for detailed explanation: dna.uga.edu/wp-content/uploads/sites/51/2019/02/Note-on-the-260_280-and-260_230-Ratios.pdf

  • @bansisharma720
    @bansisharma720 3 ปีที่แล้ว

    What about concentration how to comment on the concentration we get at ug/ul

    • @jerrysworld8242
      @jerrysworld8242 2 ปีที่แล้ว

      Same question. He didn't explain the concentration part

  • @HamdiAL-AZZANI
    @HamdiAL-AZZANI 2 ปีที่แล้ว +1

    Thank you so much dear brother