When you decide to work with a plasmid, the size of the plasmid and sequence will already be known. You can use sequence analysis tools like SnapGene Viewer to search the sequence for restriction enzyme cut sites, and that tool will show you at which bp those restriction sites are located! The question in this video assumes that that has already been analyzed, so the location of the RE cut sites are just provided to you.
@@MacBethanie Dear Bethanie, thank you so much for your kind reply. What if the sequence is unknown? I only know that I have 500bp of an amplified 16srRNA and used 27f and 519R primer. Or I can also use the snap gene to find the sequence?
I could not find those enzymes Pru1 and Pru2, but if one site is recognized by 2 enzymes, and you add both enzymes to the mixture, both will attempt to cut. If you meant Pvu1 and Pvu2, they recognize different DNA sequences so they will never recognize the same site. It is possible that the Pvu1 and Pvu2 site are right next to one another, and if both enzymes are present, they will cut at their respective sites
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Why can’t professors just be this concise? My professor spent half an hour confusing everyone and himself. And no one walked away informed
): unfortunately many professors are hired for their expertise in the field they teach (like biology), but not for their skills in education ):
Tell me about it. I swear college professors are sourced off Craigslist and Wish.
Because they are always in hurry to go home lol
You have no idea how much this has helped me. Thanks a lot ❤
I'm so glad! Thanks for telling me!
You are really good at explaining things, thank you.
Wow! I am studying for the mcat, and a similar question came up. This video helped me answer all my concerns. Thank you!
Your channel is dope as hell to me as a bio major that hates bio
Ma'am ca you tell why less travelled DNA considered to be digested while more digested DNA undigested?
how did you know where it will cut? as in at which bp?
When you decide to work with a plasmid, the size of the plasmid and sequence will already be known. You can use sequence analysis tools like SnapGene Viewer to search the sequence for restriction enzyme cut sites, and that tool will show you at which bp those restriction sites are located! The question in this video assumes that that has already been analyzed, so the location of the RE cut sites are just provided to you.
@@MacBethanie Dear Bethanie, thank you so much for your kind reply. What if the sequence is unknown?
I only know that I have 500bp of an amplified 16srRNA and used 27f and 519R primer.
Or I can also use the snap gene to find the sequence?
Thank you so much this has helped me a lot.
very good explanation
Thank you
Good stuff
what if one site is both Pru1 and Pru2?
I could not find those enzymes Pru1 and Pru2, but if one site is recognized by 2 enzymes, and you add both enzymes to the mixture, both will attempt to cut. If you meant Pvu1 and Pvu2, they recognize different DNA sequences so they will never recognize the same site. It is possible that the Pvu1 and Pvu2 site are right next to one another, and if both enzymes are present, they will cut at their respective sites
Thank you very much
Thank you so much!❤
Wow thank you so much mam
really helpful... thanks a lot!!
Thank you for this
Well explained, but how are you holding the pen 😲
I didn't know it was out of the ordinary!
@@MacBethanie oh, you're back 😅
thank you ❤❤
thank you！！😘