Thanks, mate. Actually, I've been looking for a video on how to make these to grow mushrooms for months. But all the other videos talk about using agar and pressure cookers. Things most people don't have!!!
Great info and explained in a nice straight simple way! Although I feel like a little disclaimer of being sterile and using more sterile practices when prepping these should of been said. Contamination is the #1 enemy when working with microorganisms especially in the field of mycology (Fungi/Mushrooms). All the same threw out a 👍and a sub. Interetsed in seeing your future vids!
Great video!. If i had seen this video before, our teacher said that we had to grow bacterias just using unflavoured gelatin, but that didn't work correctly. I will try by this method and wait for results! Greetings from Colombia 🇨🇴
great video... i have a question if i may ... I'm searching for a substitute for agar agar, i intend to use it to cultivate mycelium for mushroom cultivation... the gelatine works the same? i had the impression that it needed to be vegetarian like agar....?
hello, your recipe looks great HOWEVER, i cannot buy yeast extract, it is just not available anywhere in my country, i can buy yeast, which looks like a bar of butter, it is completely solid. can i replace the yeast extract with something else like honey for example if yes should add the same amount like u adding yeast that is using your proportions but with honey?
thank you for sharing Doctor.....i am doing aquaculture farm here....trying my best to make tcbs agar plate.....not very sure what to do until i see your video !!!! thanks
This video is certainly helpful for my research project! But may I ask? I have noticed that closing the lid prematurely causes my plate to evaporate water, which I only noticed AFTER swabbing the bacteria-containing cotton swab on the plate. Is it normal for the plates, after being contaminated, to be stored upside down? or do scientists normally store them upward without evaporation? Thank you so much
Right? But every channel has a slight variation of the Agar Plate recipe. Im wondering if different growths require different recipes of agar and why? No one explains the WHY of each ingredient. ie salt, yeast, bullion, sugar etc.
Hey at first thougth i think this will work fine, gelatin melt at 35 C. but the added ingrediense will increase the boilding point. Such as salt. also works the other way around and make snow melt at lower temp.
Dr, If I put something like a mini glass substrate/squared cut paper onto the yeast medium and waited for several days will the medium cover the substrate?
Thank you for making this. I literally Just mix all the ingredients (I only had regular yeast, but I read in the comment section that you said it will work just fine) in a mason jar and now it's in my pressure cooker to sterilize. I'm going to test my lion's mane mushroom liquid culture on it. Jam on! P.S. Subbed!
I think agar is the better solution as it is vegetarian made from seaweed or something like that where as gelatin is animal made product, orchid seeds best on agar I think with the right nutrient balances for growth and roots , search you tube on tissue culture , there are amazing videos out there for seed and tissue growing with orchids and other plants and fungi that might interest you , good luck .
Hi, the easiest way would be to gently heat it in a microwave. Each microwave will be different, so you will need to use trial and error. I would start with a defrost or 50% power setting for a minute or two and see if it starts to melt. Remember to top it up with water back to your original volume (before microwaving) as it will lose some as steam as it heats up Hope this helps
Some sources suggest to use agar agar powder alone and boil it in sterile water - I tried this, but it turned out a clear but cloudy colour and caused my sister’s science fair project to fail. Other sources are similar to your method, which turn out yellow. I’m confused on the difference and why salt and yeast extract are used for some but not others.
Nicely done Dr.Chobbsy. Can you tell me if it is possible to use ordinary tap water, instead of bottled water, and if I would need to add anything to compensate for the added chlorine. I know that there is stuff available for fish tank water. Great vid, as usual.
Glad you liked the video! It should be possible to use tap water, but it depends on the water regulations for your supply area. Chlorine is used as a disinfectant so it would be harmful for many of the micro-organisms, but it doesn't mean that in the large quantities that you would to potentially want to grow on a plate that nothing will grow. I believe that I live in an area that has a low level of chlorination, so I might not be able to tell is there is a difference here. The additives that you can get for fish tank water could also have some adverse effect for microbial life, so it might be worth staying away from those. Bottled water would keep remove this issue, as well as keep the mineral content as consistent as possible. Not to mention that for people living in hard water areas, it will cut down on the amount of limescale build up. The only thing i can say is to try it, and if it works for you, great!
You are right, a sterile environment is best to prevent contamination. Microwaving the gelatine mix keeps the temperature close to boiling, which is the heat treatment you are talking about. You can create a sterile environment by using a camping stove if needs be, it works in the same way as a bunsen burner and should keep a sterile environment around the burner. Wiping the surface with a bleach or high percentage alcohol will keep the surface sterile too in most cases. Limiting the amount of time the plate is open will also help prevent contamination. Like with anything, having the right controls are essential, that's why I did the "bud control plate" which will help estimate the background cells counts and types from the air and the bud. This isn't meant to be a fully scientific analytical technique video, more something you can do as an amateur at home, or to introduce kids to all of the bugs that you can't see. Again, you are correct about the contamination issue, but these plates aren't to be used in a clinical environment but a home environment, so sterile technique isn't always possible.
Hi Dr chobbsy . I enjoyed this video ,I would like to do an experiment with this what can I use other then yeast, that’s safe at home .and can we see a yeast colony under a microscope. I’m not sure what a colony is .does this mean that the yeast has grown into separate family groups. Thanks looking forward to seeing your next video
Hi, Glad you enjoyed the video! Yeast is the easiest thing to grow, and works well for beginners. What you could do is use the cotton bud to swab your phone... either on the screen or the buttons, as there is a chance that there will be something there. As for for it being safe? Well if it's there and hasn't caused you to fall ill, then it's likely to be safe. As for a colony, on the close up of the plate, you can see some small circular patches of cell growth. These individual circles are known as colonies. They are the result of on single cell being separated from the rest and dividing in that one spot. These can't really be seen under a microscope as they are rather large and the individual cells that make them normally have to be stained to be visible. Hope this helps!
Hello dr. Chobbsy!Thank you for your awesome video.I hope you see this comment because I really need your help for my project. I want to make agar plates at home and see that how many bacterias exist on objects around us. For example, i wanna see how much bacteria will grow on agar plate from a door handle, how can i do that? Should i use a cotton ball or something like that?will it even work?
Thank you. Yes any kind of yeast should grow fine on these, as will many species of bacteria and other fungi. If you have anything you want to try growing, go ahead and give it a try 😉
Hi, sorry for the long time to reply, I didn't get a notification of your comment. Anything that sets in the same way as gelatine should work OK. There are vegetarian gelatine options available now (such as the one I used). Agar powder itself is also an option which you should be able to find on an online retailer.
You can use it to grow bacteria and fungi that is on your phone, and you can use yeast extract powder instead of paste. We use it to make plates and growth media in the lab, adding roughly half as much yeast extract as salt. The extract shouldn't contain live yeast and shouldn't grow on the plate. If you use dried yeast (such as baking yeast) you may get cells surviving in the gelatine mix and growing on your plate instead of the things you want to grow. Hope this helps!
How peculiar! There is no autoclaving nor pressure cooking required, and there is no contaminant without still air box, glove box, or flow hood! How is that even possible?! Probably the culture medium is devoid of sugar. It is amazing Saccharomyces cerevisiae could grow without fermentable carbon source! About weighing liquid, I have not the slightest idea why it is 'good luck'. I weigh liquids frequently and it is easily done. As for sticky stuff, first pre-weigh spatula, tare the weighing scale, pick up sticky stuff such as honey or that yeast extract, and Bob's your uncle! It is science. Not magic. Not luck.
Just what I needed for my students to experience and make at home during lockdown and online teaching. Thank you!
You're welcome!
Do the kids grow mushrooms?
@@uttermostvids631 More like fungi and bacteria that they find on different surfaces. 🙂
@Bud Borne what type of mushroom? 😉😂
@O F good point. I'll add a comment about it on my video. Thank you
thank you but what do you do to maintain the sterility of your agar?
Thanks for the info, beginner mycologist learning about agar. Very helpful friend.
God bless u man u saved my project
do you have to store agar plates in the fridge ?
also when you put culture in them do they have to be stored in the fridge?
Thanks, mate. Actually, I've been looking for a video on how to make these to grow mushrooms for months. But all the other videos talk about using agar and pressure cookers. Things most people don't have!!!
Awesome informative video! Exactly what I was searching for with NO COMMERCIALS. I’ve liked and subscribed, good sir.👍🏼
Hello Doc. what's the salt for? (add salt- but use reduced salt yeast extract) Its the only recipe Ive seen using salt.
Could I use marmite instead of the yeast acstract?
May you tell me if your plates stay solidified at room temperature/incubator temperatures because mine surely does not.
6:58 table top is cold, temperature difference created a condensation on the glass.
Can this be used for mushroom mycelium?
Other sources suggest using beef broth? Would that be okay too?
Thank you!! Getting stuff together now ❤️
Get this man a nobel prize.
You used salt and not dextrose? No SAB or Flow Hood? What about contamination?
Great info and explained in a nice straight simple way! Although I feel like a little disclaimer of being sterile and using more sterile practices when prepping these should of been said. Contamination is the #1 enemy when working with microorganisms especially in the field of mycology (Fungi/Mushrooms). All the same threw out a 👍and a sub. Interetsed in seeing your future vids!
Great video!.
If i had seen this video before, our teacher said that we had to grow bacterias just using unflavoured gelatin, but that didn't work correctly.
I will try by this method and wait for results!
Greetings from Colombia 🇨🇴
I hope it worked OK for you!
@@Dr.Chobbsy Yes it worked! Thank you so much!
Your welcome!
Same thing happened wimme I only used some unflavoured agar but didn't work
Sir now it's cold in my country should I want to leave it in room temp or I want to incubate in any hot place?
great video... i have a question if i may ... I'm searching for a substitute for agar agar, i intend to use it to cultivate mycelium for mushroom cultivation... the gelatine works the same? i had the impression that it needed to be vegetarian like agar....?
Can I use powdered dried yeast?
Do we really need gelatin?
hello, your recipe looks great HOWEVER, i cannot buy yeast extract, it is just not available anywhere in my country, i can buy yeast, which looks like a bar of butter, it is completely solid. can i replace the yeast extract with something else like honey for example if yes should add the same amount like u adding yeast that is using your proportions but with honey?
Dr: Can I use this to do tissue culture please: orchid, hellebore.. ?
Thanks for helping me on my science experiment!
Very nice. Back yard tinkering have us guys like Tesla and the Curries.
Hi, why does my petri dish didn't solidify? I used 1tsp sugar, 1tbsp agar, 1 knorr cube and 1/2 cup of boiled water
Great video brutha. THANKS! 💯🤙🏽
thank you for sharing Doctor.....i am doing aquaculture farm here....trying my best to make tcbs agar plate.....not very sure what to do until i see your video !!!! thanks
My gelatin solidifies in fridge but when I get them out yo plate bacteria on them, the gelatin melts.
Any suggestion plz?
Can I pour the agar in a still air box? Instead of the flow hood?
Did you have to sterilize your container and/or mixing spatula?
Should be a no brainers. I didnt even know about agar but I know when growing organism a sterile environment is absolutely necessary
Hi can sea salt be used
My gelatin does not solidify at room temp. Any suggestions?
This video is certainly helpful for my research project! But may I ask? I have noticed that closing the lid prematurely causes my plate to evaporate water, which I only noticed AFTER swabbing the bacteria-containing cotton swab on the plate. Is it normal for the plates, after being contaminated, to be stored upside down? or do scientists normally store them upward without evaporation? Thank you so much
They should be stored upside down
i want to make culture media for tissue culture.... can this type of medium is helpful or not?
Great video! I was very confused until i saw this. Can I use agar 🧫powder instead of the yeast, salt and gelatin?
yes, i believe this is just an at home alternative
Very good! I was wondering where to buy the expensive agar!
This is so COOL!!!
Right? But every channel has a slight variation of the Agar Plate recipe. Im wondering if different growths require different recipes of agar and why? No one explains the WHY of each ingredient. ie salt, yeast, bullion, sugar etc.
Dr C, can one actually incubate these (home-made) dishes at 37 degrees? Or will it melt the gelatine?
Hey at first thougth i think this will work fine, gelatin melt at 35 C. but the added ingrediense will increase the boilding point. Such as salt. also works the other way around and make snow melt at lower temp.
Dr, If I put something like a mini glass substrate/squared cut paper onto the yeast medium and waited for several days will the medium cover the substrate?
Thank you for making this. I literally Just mix all the ingredients (I only had regular yeast, but I read in the comment section that you said it will work just fine) in a mason jar and now it's in my pressure cooker to sterilize. I'm going to test my lion's mane mushroom liquid culture on it. Jam on!
P.S. Subbed!
Can i use this for growing orchid seeds?
I think agar is the better solution as it is vegetarian made from seaweed or something like that where as gelatin is animal made product, orchid seeds best on agar I think with the right nutrient balances for growth and roots , search you tube on tissue culture , there are amazing videos out there for seed and tissue growing with orchids and other plants and fungi that might interest you , good luck .
What if I don't have yeast extract?
can these agar plates used for the isolation of bacteria??
Can I use brewers yeast instead of marmite? 💕
it is very interesting thank you very much
Dr if I want to turn it back liquid how much heat I will need?
Hi, the easiest way would be to gently heat it in a microwave. Each microwave will be different, so you will need to use trial and error. I would start with a defrost or 50% power setting for a minute or two and see if it starts to melt.
Remember to top it up with water back to your original volume (before microwaving) as it will lose some as steam as it heats up
Hope this helps
I can't seem to find the reduced salt yeast extract, does it need to be reduced salt? and would marmite work?
Can i use something else instead of yeast extract
Interesting. But that's not sterilised right?
why add yeast?
Some sources suggest to use agar agar powder alone and boil it in sterile water - I tried this, but it turned out a clear but cloudy colour and caused my sister’s science fair project to fail. Other sources are similar to your method, which turn out yellow. I’m confused on the difference and why salt and yeast extract are used for some but not others.
Nicely done Dr.Chobbsy. Can you tell me if it is possible to use ordinary tap water, instead of bottled water, and if I would need to add anything to compensate for the added chlorine. I know that there is stuff available for fish tank water. Great vid, as usual.
Glad you liked the video!
It should be possible to use tap water, but it depends on the water regulations for your supply area. Chlorine is used as a disinfectant so it would be harmful for many of the micro-organisms, but it doesn't mean that in the large quantities that you would to potentially want to grow on a plate that nothing will grow. I believe that I live in an area that has a low level of chlorination, so I might not be able to tell is there is a difference here. The additives that you can get for fish tank water could also have some adverse effect for microbial life, so it might be worth staying away from those.
Bottled water would keep remove this issue, as well as keep the mineral content as consistent as possible. Not to mention that for people living in hard water areas, it will cut down on the amount of limescale build up.
The only thing i can say is to try it, and if it works for you, great!
Thank you so much 💖💖💖
thank you!!
You're welcome!
very useful video!
Thank you
can i use baking soda as yeast😅
why you used yeast extract?for coloring?
its a good nutrient for the bacteria
ohh this is our experiment project
I don't understand how you can have uncontaminated dishes without sterilizing with heat and working in a sterile environment like a SAB.
You are right, a sterile environment is best to prevent contamination. Microwaving the gelatine mix keeps the temperature close to boiling, which is the heat treatment you are talking about. You can create a sterile environment by using a camping stove if needs be, it works in the same way as a bunsen burner and should keep a sterile environment around the burner. Wiping the surface with a bleach or high percentage alcohol will keep the surface sterile too in most cases.
Limiting the amount of time the plate is open will also help prevent contamination.
Like with anything, having the right controls are essential, that's why I did the "bud control plate" which will help estimate the background cells counts and types from the air and the bud.
This isn't meant to be a fully scientific analytical technique video, more something you can do as an amateur at home, or to introduce kids to all of the bugs that you can't see.
Again, you are correct about the contamination issue, but these plates aren't to be used in a clinical environment but a home environment, so sterile technique isn't always possible.
If you want contamination when growing mushroom mycelium, follow the video.
Hi Dr chobbsy . I enjoyed this video ,I would like to do an experiment with this what can I use other then yeast, that’s safe at home .and can we see a yeast colony under a microscope. I’m not sure what a colony is .does this mean that the yeast has grown into separate family groups.
Thanks looking forward to seeing your next video
Hi, Glad you enjoyed the video!
Yeast is the easiest thing to grow, and works well for beginners. What you could do is use the cotton bud to swab your phone... either on the screen or the buttons, as there is a chance that there will be something there. As for for it being safe? Well if it's there and hasn't caused you to fall ill, then it's likely to be safe.
As for a colony, on the close up of the plate, you can see some small circular patches of cell growth. These individual circles are known as colonies. They are the result of on single cell being separated from the rest and dividing in that one spot. These can't really be seen under a microscope as they are rather large and the individual cells that make them normally have to be stained to be visible.
Hope this helps!
@@Dr.Chobbsy thank you sound good.i may have a go
@Bud Borne Hi I did look at making spore prints, very interesting, thank you.
Good morning I am from the India , sir how make a pure liquid culture for the Mushrooms
Hello dr. Chobbsy!Thank you for your awesome video.I hope you see this comment because I really need your help for my project.
I want to make agar plates at home and see that how many bacterias exist on objects around us. For example, i wanna see how much bacteria will grow on agar plate from a door handle, how can i do that? Should i use a cotton ball or something like that?will it even work?
Use a cotton swab across the surface and then you whip that onto the agar mix
Links dont work
Nice Video! Subscribed. Could I use vegemite?
Vegemite should be OK. It's not something that we typically have in the UK, but it looks like it is yeast extract based.
I don't understand that you use marmite (yeast) to make the plates & then use yeast to test (contaminate) it.
Marmite is dead, it only contains nutrients while bakers yeast contains dormant or alive cells.
Bruh, can i just use a grain spawn that is 1 grain thick? I really dont need that much detail on bacteria
lol i was kinda dumb, i figured it out
Nice!
Ever use xanthine gum or gum Arabic?
Good day, I am studying for the Medical Lab Technician Exam, is this a good channel for me?
Hi ..is it the same nutrients agar ???
Yes yeast extract contains nutrients for fungi and bacteria
Pouring of the agar should be done inside a SAB or in front of a flow hood. Otherwise you will contaminate your dishes.
He's only growing yeast or mould.
Great video.
Can i used any kind of yeast??
Thank you. Yes any kind of yeast should grow fine on these, as will many species of bacteria and other fungi. If you have anything you want to try growing, go ahead and give it a try 😉
Dr. Chobbsy, what can i use instead of gelatine.
Or can you give other ingridients to make an agar plates.
Hi, sorry for the long time to reply, I didn't get a notification of your comment.
Anything that sets in the same way as gelatine should work OK. There are vegetarian gelatine options available now (such as the one I used). Agar powder itself is also an option which you should be able to find on an online retailer.
@@Dr.Chobbsy does nutritional yeast work too?
@@Dr.Chobbsy hi does active dry yeast work?
awesome thanks
are you using a plastic petri dish?
Hello Dr.Chobbsy, can I grow phone bacteria on it and can I use yeast powder instead of yeast extract paste?
You can use it to grow bacteria and fungi that is on your phone, and you can use yeast extract powder instead of paste. We use it to make plates and growth media in the lab, adding roughly half as much yeast extract as salt.
The extract shouldn't contain live yeast and shouldn't grow on the plate. If you use dried yeast (such as baking yeast) you may get cells surviving in the gelatine mix and growing on your plate instead of the things you want to grow.
Hope this helps!
@@Dr.Chobbsy This helped. Thank you!
The elon musk of microbiology
u shud make a 1 page summary
British table salt? Fuck mate, I'm screwed. I live in the US. Can you ship me some?
Elon musk is that yu lol
I just subscribed thank you for the knowledge
would a shit recipe stall out my plate? if it was "knocked" up and never grew, not even mold...how did it happen
elon?
#biology
How peculiar! There is no autoclaving nor pressure cooking required, and there is no contaminant without still air box, glove box, or flow hood! How is that even possible?! Probably the culture medium is devoid of sugar. It is amazing Saccharomyces cerevisiae could grow without fermentable carbon source!
About weighing liquid, I have not the slightest idea why it is 'good luck'. I weigh liquids frequently and it is easily done. As for sticky stuff, first pre-weigh spatula, tare the weighing scale, pick up sticky stuff such as honey or that yeast extract, and Bob's your uncle! It is science. Not magic. Not luck.
Thank you bootleg elon musk you helped me
You say these are agar plates but they're not, they're gelatin.
Gelatin is ok too, agar is just better.
Istruzioni poco chiare
Ho iniziato la pandemia di covidd
you do know Agar is an actual thing, and your not using it, so these are not agar plates.
Do you know vegetable gelatine is usually agar agar?
Elon musk?
Looks like Elon Musk. 🤔
No sceptic procedures or steralization of agar mixture prior to pouring on plates. what a joke
And you can't spell, so who's the joke? Perhaps if you paid attention to what he was doing and why, you might understand.
Blink twice if you need help