In Situ Hybridization for BEGINNERS

[@aliiranban8871]

I watched a this for a college presentation and it's nearly completely what i needed to know about it!
I eagerly look forward for your new content.
Maybe you could cover more techniques in microscopy.

[@maryg5941]

Super cool video! Watched it as a resfresher for a job Interview. You did a great job! 😍

[@surayuthpintawong8332]

Thanks, the way you described this material is so good.

[@codeblue3228]

Wonderful explanation, please make a video on whole mount insitu hybridization.

[@SirPlantsaLot]

'gene for people who like hummus' loooool, you're great at explaining things, thank you!

[@yelkayap7813]

thank you so much for this! i feel like i can pass my exams tomorrow ^^

[@tah4155]

Wow! Amazing teacher! thank you for this video :))

[@biofilia_]

Very clear explanation. Thanks!

[@wide_eyed_corpsebride]

thanks a lot for the video! really informative

[@nileshsharma0212]

Please make more videos about techniques which you use for your research

[@Vislumbre.]

Heyyy, that was a great explanation, thank you very much!!

[@airicsnider7572]

Thanks, Comrade.

[@imethan-youtubetips1770]

7:25 this was very informative. Also good editing. Thumbs up from a fellow creator 😅

[@realizingresearch4194]

Thanks! I'm really glad you liked it.

[@farahal-najjar6480]

Thank you!

[@anajuliamacedo6239]

YOU SAVED ME

[@anupshrestha431]

thanks for the clear explaination. Bit off topic but can you share the source of the background music? I think it made the video much nicer when it started around 6:30.

[@tonysarris668]

Thanks for the video. Could flow cytometry be used for the detection of these probes?

[@samuellevac1919]

What do you mean by temperature sensitive? More specifically how does the cold affect it?

[@realizingresearch4194]

Great question! Thanks for asking! The primary effect temperature has on this type of reaction is changing the binding between the probe and your DNA/RNA. The DNA and probe will not bind at too high of temperatures and too low of temperatures, say below 40C/104F, you have nonspecific binding. This is similar to where I described your probe binding to other RNA/DNA. So you need to find the optimum temperature for your reaction based on your tissue and probe. Does that make sense?

[@anapalacios8452]

¿What should I use in order to quantify the expression of the genes?. Thanks!

[@realizingresearch4194]

There are a few options, I think the best and most widely used tool is qRT-PCR.

[@jonnyjaap]

Nice video and you have a lot of positive energy, cool!
You could talk about qPCR, and it can be used for quantification, right ?

[@realizingresearch4194]

I am definitely gonna do a video on qPCR. I just wasn't sure when. I'll move it up. Thanks for the nice comment!

[@samuelfink8569]

I wish I exhibited the "Super Cool Gene" :(

[@realizingresearch4194]

I mean, you did watch the video....

[@addieandlogansyaya]

I wonder if she has sibling competition issues

[@realizingresearch4194]

Only in video games and school grades and life. 😁

[@vincent70]

You're funny

[@amruthatk6371]

THANK YOU 🤝