Troubleshooting Polymerase Chain Reactions

This video explores different ways to troubleshoot problems that may arise when performing a polymerase chain reaction (PCR). A PCR is an experimental technique commonly used in the lab during undergraduate classes. However, problems may arise when performing this technique. This video examines4 different problems which may arise, while primarily focusing on troubleshooting weak/faint bands. The other 3 problems this video looks at is unexpected/nonspecific bands, missing/no bands, and smeared bands.
This video was made by McMaster University students Ravneet Sidhu, Simran Chima, Julia Steriopoilos, and Parmida Behniain collaboration with the Demystifying Research McMaster Program.
Copyright McMaster University 2022
References
Martin, K. (n.d.).Troubleshooting PCR part three: Solutions for weak bands and smearing.GoldBio. Retrieved from goldbio.com/articles/article/...
PCR setup-six critical components to consider. Thermo Fisher Scientific -US. (n.d.). Retrieved from www.thermofisher.com/ca/en/ho...
PCR troubleshooting. Bio Rad. (2022). Retrieved from www.bio-rad.com/fr-ca/applica...
Polymerase chain reaction (PCR) fact sheet. Genome.gov. (2020, August 17). Retrieved from www.genome.gov/about-genomics...
Polymerase chain reaction facts for kids. Polymerase chain reaction Facts for Kids. (2022, September 23). Retrieved from kids.kiddle.co/Polymerase_cha...

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Thank you. Keep going ❤