PCR & qPCR Troubleshooting - Part 4

Part 4 of a 4 part series on Polymerase Chain Reaction (PCR) provided by Dr. Lexa Scupham with the Center for Veterinary Biologics (CVB)

[@niroshaluthge4685]

I watched the entire series of videos on PCR as well as the lab demonstration of qPCR. Really great content for beginners as well as experienced users. Thank you so much Dr. Scupham and USDA-APHIS!

[@gbheo3571]

Dr. Lexa, It is really helpful to newcomers on molecular technique. I will share this video to my colleagues. thank you.

[@tawfiqalbalawi3617]

Thank you so much for this lecture! It was help me to detecting my PCR error

[@godfreyogulla5682]

Excellent discourse!

[@GAT777]

I got amplification using one dna template...but when I repeated the same reaction with same template and same conditions I didn't get the bands.....can anyone please help to know the reason?.. please