Sodium lauryl sulfate (SLS) and sodium dodecyl sulfate (SDS) are detergents commonly used to disrupt cell membranes in various experiments. However, they are not ideal for DNA isolation practicals for several reasons: 1. **Protein Denaturation:** SLS and SDS are strong protein denaturants. While they effectively break down cell membranes, they also denature proteins, making it challenging to isolate intact, functional DNA. 2. **Enzyme Inhibition:** DNA isolation often involves enzymes that are sensitive to denaturation by these detergents. The use of SLS or SDS can inhibit the activity of these enzymes, affecting the overall success of the isolation process. 3. **Precipitation Issues:** SLS and SDS can interfere with DNA precipitation steps. They may remain in the solution and affect the purity of the isolated DNA. For DNA isolation, milder detergents, such as Triton X-100, are preferred. These detergents effectively disrupt cell membranes while minimizing interference with DNA structure and enzyme activity, resulting in better-quality DNA isolation.
😂😂😂.. over Indian and our student not beginner ... legend everything's make jugaad... 😂... Bohot sahi h.. superb dear.. desi jugad.. creativity in our mind 🤣🤣
Bro your explanation is just wow..everything is crystal clear 😊 thank you so much..
BTW.. tomorrow is my practical 😅😂❤
Mine too 😂
Same😂
Thanks sir ji for this amazing video
Kya casual practical hai bosssss
Viva ke bare me
kuch bta dijiye sirr
Bhaiya pure world me nhi hai iss se badiya vedeo ❤❤
App sab logo ka pyar he 😊😊
Kl mera practical hai ye bht kaam aayega
Kaisa gaya
@@csmeena1 mst
Thank you for your efforts sir❤❤
Everything is now crystal clear Tq so much🤌🌼✨...
Bharat sir jab sath Ho to darne ka nahin macha ke aane ka 😂..
@@LetMeExplainnowbharatpanwar yess sir Thanks a lot 🥰✨....
❤
ASC pe padte ho kya
@@ShivPatil333 nahi..
Everything is crystal clear ❤❤❤❤❤ thank you sir 🙏
Ek baar mai hi clear ho gaya..
1 hour before my biology practical 😂❤
Nice🎉
❤🎉🎉🎉🎉mjaaaaaa aaagyaaaaaaa
Are... Wa. Sun . Ke.. mja . Aa gya... 😂
@@LetMeExplainnowbharatpanwar aaaaaap kya aiiims m hoi???
Love from Kerala ❤❤
Thnk uuuu bossss❤❤❤❤❤❤❤
Very nice ❤❤
Plz explain mitosis in onion root tip experiment also
All ready uploaded
Thank you sir ❤
Thankyou sir crystal clear😄😃😃😃
@Rishu_kumar1734 yes
Nice sir, why you can't use sodium laureal sulphate or sodium dodecyl sulphate.
Sodium lauryl sulfate (SLS) and sodium dodecyl sulfate (SDS) are detergents commonly used to disrupt cell membranes in various experiments. However, they are not ideal for DNA isolation practicals for several reasons:
1. **Protein Denaturation:** SLS and SDS are strong protein denaturants. While they effectively break down cell membranes, they also denature proteins, making it challenging to isolate intact, functional DNA.
2. **Enzyme Inhibition:** DNA isolation often involves enzymes that are sensitive to denaturation by these detergents. The use of SLS or SDS can inhibit the activity of these enzymes, affecting the overall success of the isolation process.
3. **Precipitation Issues:** SLS and SDS can interfere with DNA precipitation steps. They may remain in the solution and affect the purity of the isolated DNA.
For DNA isolation, milder detergents, such as Triton X-100, are preferred. These detergents effectively disrupt cell membranes while minimizing interference with DNA structure and enzyme activity, resulting in better-quality DNA isolation.
😂boss 💪 thank you tomorrow is my practical exam
All the best
@@LetMeExplainnowbharatpanwar thank you boss 💪
Bhaii majja aaya achhe se samja 💋
😂😂😂😂😂😂.. mja he to chiye . 😂
Can we add papain extract from papaya? Is it soluble/insoluble in banana extract
Yes .
CAN WE USE SAVLON
Yes
Mein bsc student hoon or hum v lab pe jugad se dna isolation kiye the 😅 or hum raw papaya juice liye the replace protease .
😂😂😂.. over Indian and our student not beginner ... legend everything's make jugaad... 😂...
Bohot sahi h.. superb dear.. desi jugad.. creativity in our mind 🤣🤣
Idea to bhut hai banana ko crush karny ka but examiner se dar lagta hai😅😅
Examiner ki
Can we use ezee detergent liquide in place of washing deargent?
Yes u can use
@@LetMeExplainnowbharatpanwar sir what is use of spinach papaya in this experiment?
Salt bhi use karte hai kya ?
Yes
kal ke liye help hogyi
Nic
Easy is best 😊
❤❤
Onion Root please sir
Already uploaded
Kal ka practical h bhai😂
Lage raho munna bhai 😂😂😂
Shai peg banaya sir😂
Hamko papaya se karwate ,crush hi na hota aache se
Are koi bhi fruits le skte ho
kela kha liya tha💀💀💀
😂😂😂😂😂😂 Sai kiya...
Thank you sir ❤
😊