HPLC problems with very polar molecules.

🔬 Welcome to the world of HPLC columns, specifically focusing on the 'Polar Embedded Phase' technology! 🔍 In this video, we unravel the intricacies of these highly efficient columns. If you're a scientist, researcher, or anyone interested in analytical chemistry, this video is for you!
🌊 What Are Polar Embedded Phases? 🌊
Discover the unique 'Polar Embedded Phase' columns in HPLC technology. We explain why major companies are adopting this technology and how to identify these columns by their water-themed names like Aqua, Aquasil, Hydro, Hydroselect, and AQ.
🧪 Design & Composition 🧪
We break down the unique design of these columns, predominantly a type of C18 column, but with a twist! Learn about the polar linkage at the base of the carbon structure, be it carbamate or amide, and understand why this feature is crucial for their performance in 100% pure water.
💧 Functionality in Water 💧
Find out how these columns stand out with their ability to operate in 100% water environments, maintaining efficiency throughout the day. This section is key for those interested in analyzing highly polar molecules.
🔬 Application for Weak Acids 🔬
Learn about the versatility of these columns, particularly in separating weak acids like acetic acid, which are typically challenging to analyze via reverse phase.
🔄 Versatility of Use 🔄
Explore the dual capability of these columns, handling both polar and non-polar compounds and their ability to work with up to 100% organic solvents.
🔎 Comparison with Other Columns 🔎
Understand why, despite their efficiency, polar embedded phase columns are often the secondary choice compared to high-quality base-deactivated C18 columns.
🧐 Polar Endcap Phases 🧐
We also delve into the polar endcap phases, a similar yet distinct technology, and how they complement the polar embedded phases.
👩‍🔬 Join us for this informative session that will enhance your understanding of modern chromatographic columns and their applications in diverse analytical scenarios. Perfect for experts and novices alike!
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