Thank you so much for this clearly explained video. Although it would have been much better if the video speed was a bit faster and had a voice recording too! But super easy to understand, thanks AGAIN!!!!!
It is all clear with the polar particles.But does a non-polar stationary phase really retain non-polar molecules because they have affinity? What force causes such affinity then?
+Surajcxs c.singh All molecular interactions are fundamentally electrostatic in nature and can be described by some variation of Coulomb's Law. Non-polar molecules can be polarized momentaneously, for example by the repulsion of their own negatively charged electron clouds when they are located nearby to each other, so the oscillating dipoles sense each other and couple. This is a favorable attractive electrostatic interaction known as dispersive interactions. Meanwhile polar molecules in the column are experiencing stronger interactions between them. That is why polar and non-polar compounds tend to repeal each other, as you can see trying to mix some water (polar) and vegetable oil (non-polar) in a glass. :-)
Hey everyone, im a little unsure about this. For reverse phase hplc, if the non polar moves slower through the stationary phase, what will be affected?
very simple yet to the point and clear! thank you!
Thank you so much! This explains the concept in a very clear and easy to understand way. Keep it up sir!
Very helpful now i can finish my analytical chemistry assignment :)
Thank you so much for this clearly explained video. Although it would have been much better if the video speed was a bit faster and had a voice recording too! But super easy to understand, thanks AGAIN!!!!!
Clear explanation... but I prefer it to be with voice.. anyway thanks 😍
It is all clear with the polar particles.But does a non-polar stationary phase really retain non-polar molecules because they have affinity? What force causes such affinity then?
Excellent animation! Very clear! Thanks!!
Thanks alot for saving my time & providing mh the best explanation possible ...💝
Better than some professors I would say
Awesome video! easy to understand. Thanks alot
This was extremely useful! Thank you
Thank you for your very clear explanation.
Simple and useful presentation
Thank you so much sir it's very easy to understand 😊
This is just awesome, pls keep on making videos on everything. and it will have success
Awsome, you saved my time👍👍
Great video. But wish there was audio.
Very nice animation sir....well explained
So easy. Thank you!
Very good explanation, thank u sir
Great video thanks a lot !
Nice video!! I understood
thank so much! it helps me!
Well done it is so useful
superb animation
Saved my life
Thank you so Much, this video is really helpful
Put some more video on hplc... nice video.. easy to understand...!!!
Thank you for this video
One question when u have HPLC -Normal Phase your solvent can be hexane cause this molecule is Non Polar like Mobile Phase isn´t it?
Well explained sir thank you so much
Thanks for the clear explanation...
thats dont have voice
Hey i was wondering wha Programm do u use for your animations ?
Thank you very much
I have one question sir Is that
Is Stationary phase and identifying spot both are having same state like polar or nonpolar....?
why one of the main use of normal-phase chromatography is for separation of isomers???
thanks so much keep it up ....
easy to understand d concept ...thnku so much ...will u pls upload more videos relates to hplc
Thank you sir
thank you it's clear
this is simple as simple
thank you very much ^^
شكراااا
Is the sample to be analyzed in the normal phase polar or non-polar? And in the reverse phase?
sir but i don't understand how there will be attraction between two non polar molecules.
+Surajcxs c.singh Like dissolves in like, they are not interacting based on charge.
+Surajcxs c.singh All molecular interactions are fundamentally electrostatic in nature and can be described by some variation of Coulomb's Law. Non-polar molecules can be polarized momentaneously, for example by the repulsion of their own negatively charged electron clouds when they are located nearby to each other, so the oscillating dipoles sense each other and couple. This is a favorable attractive electrostatic interaction known as dispersive interactions. Meanwhile polar molecules in the column are experiencing stronger interactions between them. That is why polar and non-polar compounds tend to repeal each other, as you can see trying to mix some water (polar) and vegetable oil (non-polar) in a glass. :-)
oil(non polar) still will be separated from water(polar).
Hey everyone, im a little unsure about this. For reverse phase hplc, if the non polar moves slower through the stationary phase, what will be affected?
the speed of polar particles which are getting out will be affected
gerry tan why the polar bind then polar??
Thanks so much..
Good
this is good video yet there is no sound?? Thanks
good
thankyou muchas gracias danke schön terimakasih
Mujhe bhi samjh aa gya
Am I the only one not getting any audio?
So much to be said for the difference between normal and reversed phase HPLC, but this video doesn't say shit!!
Bless u
very nice video...thanx alick from zambia
who else was caught by todays ad
simple and effective
No voice
K
Poor audio quality