HPLC - Normal Phase vs Reverse Phase HPLC - Animated

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Basic info about Normal Phase and Reverse Phase HPLC.
There are two variants in use in HPLC
01. Normal Phase - HPLC
02. Reverse Phase - HPLC
The 2 variants depends on the polarity of the Mobile Phase and the Stationary Phase.
Learn the basic concept of High Pressure Liquid Chromatography
01. Introduction
• HPLC - High Perfermanc...
02. The Mobile Phase
• HPLC - What is the fun...
03. The Stationary Phase
• HPLC - The Stationary ...
04. Normal Phase HPLC vs Reverse Phase HPLC
• HPLC - Normal Phase v...
05. HPLC Isocratic vs Gradient analysis
• HPLC - Isocratic vs Gr...
06. HPLC - UV-VIS detection of analytes
• HPLC - UV / VIS detect...
07. HPLC - How to read a chromatogram?
• HPLC - How to read Chr...
08. What is the difference between HPLC and GC?
• HPLC and GC - what is ...

[@marina15554]

very simple yet to the point and clear! thank you!

[@faridyusuf1841]

Awesome video! easy to understand. Thanks alot

[@pablocardella5930]

Excellent animation! Very clear! Thanks!!

[@claral2533]

Thank you so much! This explains the concept in a very clear and easy to understand way. Keep it up sir!

[@dhruvmini01]

This was extremely useful! Thank you

[@reececowell6642]

Very helpful now i can finish my analytical chemistry assignment :)

[@32barber89]

Thank you so much for this clearly explained video. Although it would have been much better if the video speed was a bit faster and had a voice recording too! But super easy to understand, thanks AGAIN!!!!!

[@KriiZInQ]

This is just awesome, pls keep on making videos on everything. and it will have success

[@mustafaaslan5910]

Great video thanks a lot !

[@uyenst]

Thank you for your very clear explanation.

[@CamilaRodriguez-hv7ls]

So easy. Thank you!

[@javeriasaeed2074]

Thanks alot for saving my time & providing mh the best explanation possible ...💝

[@Red00731]

Hey i was wondering wha Programm do u use for your animations ?

[@agentgunnso]

thank so much! it helps me!

[@wessammohamed9235]

Clear explanation... but I prefer it to be with voice.. anyway thanks 😍

[@En_hausse55]

Thank you so Much, this video is really helpful

[@prathz1065]

Awsome, you saved my time👍👍

[@pradyumnauchale1526]

Very nice animation sir....well explained

[@miriamhernandezgarcia7238]

Nice video!! I understood

[@myworldofinstrumentation6523]

Simple and useful presentation

[@Safrout007]

thank you very much ^^

[@chinna2680]

Thank you so much sir it's very easy to understand 😊

[@Andreijkmuyt]

It is all clear with the polar particles.But does a non-polar stationary phase really retain non-polar molecules because they have affinity? What force causes such affinity then?

[@PrabuddhaSachan]

Put some more video on hplc... nice video.. easy to understand...!!!

[@sabavatmamatha9389]

Thank you for this video

[@zahrazara4492]

thank you it's clear

[@aisharai4491]

superb animation

[@beatrizfreitas9034]

Is the sample to be analyzed in the normal phase polar or non-polar? And in the reverse phase?

[@The_real_Sumaya]

Well done it is so useful

[@nakkellasatyanarayana9709]

Very good explanation, thank u sir

[@pankajpundir4497]

easy to understand d concept ...thnku so much ...will u pls upload more videos relates to hplc

[@shaikabdullah..]

Well explained sir thank you so much

[@berraozcan3976]

Saved my life

[@mehmoodhassan5355]

thanks so much keep it up ....

[@Fl0wright]

Great video. But wish there was audio.

[@khloudmoh1030]

Thanks so much..

[@user-fb4ee6ph1c]

Thank you very much

[@catarinalemos1841]

One question when u have HPLC -Normal Phase your solvent can be hexane cause this molecule is Non Polar like Mobile Phase isn´t it?

[@ChemistryTipsTricks]

Thank you sir

[@dhamot.k1561]

this is simple as simple

[@ironlotuses2162]

Better than some professors I would say

[@kanapathysima9550]

Thanks for the clear explanation...

[@leilasvr7144]

thats dont have voice

[@basantmohamed4610]

why one of the main use of normal-phase chromatography is for separation of isomers???

[@darshandarji8440]

I have one question sir Is that
Is Stationary phase and identifying spot both are having same state like polar or nonpolar....?

[@gerrytan8164]

Hey everyone, im a little unsure about this. For reverse phase hplc, if the non polar moves slower through the stationary phase, what will be affected?

[@zahrazara4492]

the speed of polar particles which are getting out will be affected

[@aposvlah1855]

gerry tan why the polar bind then polar??

[@elenagouitel6041]

this is good video yet there is no sound?? Thanks

[@zaouiamina5799]

شكراااا

[@pharmasolutionpakistan7668]

Good

[@mobarokkorim7902]

good

[@DeepanMadan]

simple and effective

[@appleseeds5603]

Bless u

[@alicksinyangwe1838]

very nice video...thanx alick from zambia

[@Surajcxscsingh]

sir but i don't understand how there will be attraction between two non polar molecules.

[@Dealcapon97]

+Surajcxs c.singh Like dissolves in like, they are not interacting based on charge.

[@easylearning981]

+Surajcxs c.singh All molecular interactions are fundamentally electrostatic in nature and can be described by some variation of Coulomb's Law. Non-polar molecules can be polarized momentaneously, for example by the repulsion of their own negatively charged electron clouds when they are located nearby to each other, so the oscillating dipoles sense each other and couple. This is a favorable attractive electrostatic interaction known as dispersive interactions. Meanwhile polar molecules in the column are experiencing stronger interactions between them. That is why polar and non-polar compounds tend to repeal each other, as you can see trying to mix some water (polar) and vegetable oil (non-polar) in a glass. :-)

[@farahzahar7830]

oil(non polar) still will be separated from water(polar).

[@theoryvan5784]

thankyou muchas gracias danke schön terimakasih

[@neetudhaker8735]

Mujhe bhi samjh aa gya

[@hasridhara]

No voice

[@Bripirate]

So much to be said for the difference between normal and reversed phase HPLC, but this video doesn't say shit!!

[@chetangoudr3396]

K

[@kayjay1088]

Poor audio quality