William Shih (Harvard) Part 1: Nanofabrication via DNA Origami
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- เผยแพร่เมื่อ 8 มิ.ย. 2024
- www.ibiology.org/biophysics/n...
Talk Overview: Shih describes how DNA can be used as a building material to construct nanoscale objects. A long strand of DNA can be made to fold into a flat "sheet" by introducing short oligonucleotides that base pair with the long DNA strand and form crossovers to hold the structure together. Engineering complementary sticky ends onto some of the "sheets" allows the DNA to self-assemble into 2-dimensional shapes. Ultimately, these flat sheets of DNA can be designed so that they will self assemble into 3-D nanostructures or "DNA origami", even larger than a ribosome.
In Part 2, Shih describes a complementary DNA building technique that uses DNA "bricks" rather than "sheets" to build 3-dimensional nanostructures.
In the last section of his talk, Shih describes some of the practical uses of DNA-nanostructures. For example, DNA nanotube liquid crystals can be used to help align proteins in NMR experiments leading to improved atomic-resolution structures. DNA nanostructures may also prove useful as a mechanism to deliver therapeutics to cells and Shih and his colleagues have investigated the ability of nanostructures of different sizes and shapes to be taken up by cells.
Speaker Biography: William Shih is an Associate Professor in the Department of Biological Chemistry and Molecular Pharmacology at Harvard Medical School and the Department of Cancer Biology at the Dana-Farber Cancer Institute. He was a graduate student in the Department of Biochemistry at Stanford University, and a postdoctoral fellow in the Department of Chemistry and Molecular Biology at the Scripps Research Institute.
William's lab investigates methods to design self-assembling nanostructures made from DNA. This process, called "DNA Origami", could be used to develop absolutely tiny devices for use in biomedical applications . For his research, William received the New Innovator Award from the National Institutes of Health, in 2008. - วิทยาศาสตร์และเทคโนโลยี
Thank you so much for this video, it is so much easier to understand than reading journal articles. lol
True
wow happy 10 years on this series! I just stumbled upon these videos today coming from a separate 2021 presentation by Dr. Shih. I'm curious just how far this research must have come a decade later
8:00 Holliday junction.
18:00 Layered folds.
26:55 Frameworks.
34:34 Structural bending.
Very nice lectures and channel. Thanks so much for making these!
Now it makes me curious as to if you could design a "DNA crystal" of sorts that could be produced in large quantities and placed inside a liquid. In this liquid, it would form colloids, kinda like fat molecules in milk. Then the dna crystal could react to different stimuli in the liquid or directly on itself and change properties of both itself and the liquid it is suspended in. What if it were suspended in a non-newtonian fluid and could cause it to harden by vibrating when exposed to some sort of stimuli. Or a liquid that can reactivly change colors based on stimuli by changing the shapes of the dna crystals to affect the albedo of the crystals, and thus affecting the overall color of the liquid. Maybe it could be used in display tech similar to how we have LCDs, but in a 3D format. That would be neat. Sorry for the random stuff here, I just find things like this interesting and wish we had more focus on these types of research. I feel like we should first understand the small, then use the small to build bigger things rather than keep trying to build bigger things. By understanding them we could make way more complex, yet more effecient machinery and the such. Although it does make me curious what these kinds of machinery would look like. Would be horrifying if they almost seemed organic lol.
Do you use a ligase to ligate the sticky ends after letting the structures assemble? I imagine this would considerably improve stability and strength.
It's quite interesting that the frame of the figures is designed in purpose to look like an album or origami art work
I HAVE A QUESTION:
HOW DNA PATTERNS ARE PROGRAMMED?
(good job with the video)
what classes do you have to take as an undergrade to go into this field? Also what engineering major will lead to this field?
Nanotechnology/Nanobiology/Life Science and Technology I guess
How does this structure fold. Notes for later
Interesting stuff
a whole new world
I need you, you need me!
What the applications even
this is literally the building blocks of bio nano robotics the numbers of applications with it is unimaginable.
I hope these discoveries never become applicable within the military industrial complex once the technology matures.
Sincerely,
Useg Diaz-Granados
the first use will likely be in either medicine or in microelectronics. So yes they will have applications to the military. The kind of horror weapons you are likely thinking about though are banned as biological warfare.
OversightEntertainment they did @FBI
MrGoatflakes Still used by #MSN “News” Producers....
Science shows their Works...
Wishful thinking. Warfare whether characterized as war against illness, sickness, and disease, or a nations enemies (National Defense/Offensive purposes), is par for the course.
@@MrGoatflakes
Banning always works.
*Sarcasm alert*
they did a nano emoji lmao
i went toohb harvard tooh, my parents were rich they made teir moneey in that dry clrening buziness
bill shee 😄
my thang is dirty mamoneeters
I invite women to play with my head capsid
I'm this guy, i got my skin darkened and went undercover mark tyksinski upton - the government is very worried
this man needs to get his green card checked
Please stop!
Why?