Instead of blood agar could you just use a MSA agar and check for the yellowing of manitol fermentation to verify if its S. aureus instead of using blood agar and all of the other tests.
Hi, I have a question: since S. aureus lives in the mucous membranes of human nasal cavities (not only), I could obtain some mucus and put it in 12ml of Mannitol Salt Broth and incubate it for 24 hours at 37°C. After incubation I could take a few scoops of culture broth and do an isolation streak on blood agar and incubate again for 24/48h at 37°C. After incubation I would look for beta hemolytic colonies with a rounded, golden appearance. Would be fine.
@@simcy1720 I really have to understand what you are asking in order to answer you. If you are asking how to "culture" bacteria in the meaning of "growing" bacteria, then you just put the bacteria into the right medium and condition, they will become more in number, you can call the process "enrichment" or "amplify" Or you are talking about getting the specify bacteria you want, then it would be the process of "isolation", so which one do you mean? Or you are asking something else?
Thankyousomuch for this video! It will help me alot for my thesis ointment for staphylococcal pyoderma in dogs!
Thank you so much this is very helpful for my rsearch♡♡
Great video! Very well done!
Thank you.
Interesting and very educational too, awesome video too. Question? I heard and that you can also get staphylococcus also from animals nostrils too?
Yes, staphylococcus are common bacteria that live on animals.
Instead of blood agar as the first step for isolation, can I directly use mannitol salt agar?
Yes, you can change the order of the medium, but MSA is a bit difficult to pick colony than BAP.
@@diymicrobiology378Thanks!
Hello
Could you please tell how much time does S.aureus take to reach the exponential phase if inoculated in Mueller Hinton broth
I would say 4 hr, but it really depend on your strain.
Instead of blood agar could you just use a MSA agar and check for the yellowing of manitol fermentation to verify if its S. aureus instead of using blood agar and all of the other tests.
Is that a question or?
Hi, I have a question: since S. aureus lives in the mucous membranes of human nasal cavities (not only), I could obtain some mucus and put it in 12ml of Mannitol Salt Broth and incubate it for 24 hours at 37°C. After incubation I could take a few scoops of culture broth and do an isolation streak on blood agar and incubate again for 24/48h at 37°C. After incubation I would look for beta hemolytic colonies with a rounded, golden appearance. Would be fine.
Enrichment first is one way of doing it, it will be fine.
Can u please tell me about the price of blood agar plate please
mine are about $1.5/plate, buy 200 at a time, I doubt it would be the same at where you live.
@@diymicrobiology378 From where do you buy them? Just a curiosity, maybe I'll manage to buy them cheepier.
@@JovanKarajovanoski I get them from local supplier.
Very interesting.. question, what method did you use to culture the s. staph bacteria?
What do you mean? You just put the bacteria in the right medium and condition and it will grow on it's own.
@@diymicrobiology378 Is that the agar well diffusion method, sir?
@@simcy1720 No, it is not, totally different.
@@diymicrobiology378 Is there a name for that, sir?
@@simcy1720 I really have to understand what you are asking in order to answer you. If you are asking how to "culture" bacteria in the meaning of "growing" bacteria, then you just put the bacteria into the right medium and condition, they will become more in number, you can call the process "enrichment" or "amplify" Or you are talking about getting the specify bacteria you want, then it would be the process of "isolation", so which one do you mean? Or you are asking something else?
I got an ear infection from this becteria hurts so much
Yes, S. aureus is an opportunistic pathogen, it will infect human if given the chance.
I have in my nose and it causes polyps, AB helps but a little, I'll try bacteriophages.