Microbiological Techniques: Membrane Filtration Technique (MFT)

For Bacteriology I, Microbial Ecology and Marine and Estuarine Microbiology. Suitable for cleaner waters where large volumes can be processed therefore giving a very good detection limit.
I have published an ebook "Practical Bacteriology" to guide readers on the culturing, enumeration and identification of bacteria. For the enumeration, I cover both culture dependent (pour plating, spread plating, most probable number, membrane filtration technique) and culture independent methods. You can buy it from here:
www.lulu.com/e...

[@MichelleMunguia-figueroa]

Hi! Great video. What's the correct way to autoclave the filter paper? Do you have to soak the filter membrane in distilled water first?

[@LeeChoonWeng]

We wrap them in aluminium foil and then autoclave them. Ensure they are dry before using them.

[@MichelleMunguia-figueroa]

Thanks for the reply! Do you wrap the whole petri dish or just the filter papers?@@LeeChoonWeng

[@LeeChoonWeng]

@@MichelleMunguia-figueroa wrap the filter papers. When need, aseptically use cleaned forceps to pick the filter and place them on top of filter base