Binary Vector | Agrobacterium | Transformation in plants | Ti plasmid | Biotechnology |

The binary vector is a two plasmid vector system created by modifying the Ti plasmid from Agrobacterium. One of the plasmid in the pair carries DNA insert and other carries the vir genes for the transfer of the insert from Agrobacterium to plants. A quick explanation of the whole procedure for students of BSc & MSc. Happy Learning!!

[@masoumehshekarriz]

Thanks for the simplicity✨️

[@lopamudrachandra2493]

All steps are clearly explained in a crisp manner. Keep those videos coming

[@dureremu5897]

Concise and clear, this is really helpful!

[@rohithragesh392]

Explained in the most precise and effective manner.
Very Helpful.

[@peerzadazubairahmad464]

Best explanation 👍👍👍
While watching this vedio i felt like I am doing all the steps you mentioned in the lab. Thanks and keep🔥🔥🔥 up

[@syyylvo]

Appreciate the conciseness!

[@mominjaved7616]

Well done.. Allahhh gave u a very good explanation method...

[@vickycheong7489]

thanks for making some complex ideas easy to understand, this video was very appreciateed :)

[@glendalizeth4552]

Thank You very much, it was a very informative and visual video. Keep up the good work.

[@sheikhwali1225]

Informative
👌👍

[@siddhi.d5364]

Nicely explained 👌 thanks!

[@ARN8250]

Thank u.
It's helpful.

[@ishitatalwar4502]

Very well explained...😊😊

[@JetsoiSA-B]

Precise explanation 😌

[@rachanapandey9384]

extremely helpful..

[@kishangurjar9653]

Worth it to see. Simple easy and effective way of explanation ❤️

[@vidushisharma9768]

🙏

[@kishangurjar9653]

@@vidushisharma9768 are are😂

[@shaiestacuk2571]

Nicely explained..

[@cruelclown178]

Very helpful

[@albahrihardwaredipshowroom9695]

Very nice

[@showkathussain2552]

Excellent

[@prakashae3997]

Thanks sis

[@mukeshacharya7994]

thank you 🤗🤗😇

[@PriyanshuSharma-cj6jv]

❤❤❤❤

[@ruhamaaamer2989]

Good explanation overall but I have a question. The helper plasmid only contains Vir gene then how does kanR develop in it. And how it also grows on media containing kanamycin?

[@Shivanisingh-vu1rt]

Nice

[@nilminijayalath8177]

very good. Thanks. Could you make a video preparing helper plasmid and disarmed plasmid to prepare final agrobacterium solution step by step in detailed manner

[@qASheducationcontent]

Thank you for the appreciation!! Sure, your requests will be uploaded soon.

[@akilgupta7088]

Good

[@veruschkapate5115]

Extremely helping thank you. Question - Is this process only specific to neomycin-resistant gene transfer or can it be the same for inducing other crop resistances, ie insect pest resistance using the Bt protein into maize crop? Is the process very different then?

[@qASheducationcontent]

Yes, the Ti plasmid vector systems can be used to induce insect resistance, conditional resistance, and metabolite production in plants. The process remains same, only the gene of interest changes.

[@Taiaala]

Can this experiment be done in vivo?

[@mominjaved7616]

Just 1 question k vir genes helper k or DNA interest Mini TI plasmid sy jaein gy plant cell mai????

[@hajragupta134]

Yes, you're right. Vir genes on helper plasmid induce karenge transfer dna of interest ka from mini Ti to plant

[@mominjaved7616]

Thank u sooo muchhhh.. U r veryyyyy nice& gr8

[@somyatiwari4711]

what if plant specific selectable marker in a Ti plasmid binary vector has been disrupted during the recombinant DNA formation with a specific DNA insert .whether this Ti-plasmid Still be used for plant transformation ?

[@qASheducationcontent]

Hello Somya,
So the two plasmids do not undergo recombination event. They stay as individual entities. If you're talking about the construction of the vector and adding various sequences in continuity, then that is taken care by sites of homology and complementary base pairs. Also, use of linkers and adaptors can help in joining two sequences.

[@mominjaved7616]

V.gdddd🌷🌷💓💓... Is this video present
Insertion of foreign DNA into TDna ???

[@qASheducationcontent]

So this video is about insertion of foreign dna present in tdna from Agrobacterium to plant. The video for cloning of foreign DNA in TDNA is not included in this dear.

[@chanchalpatidar5752]

Binary vector hindi me video all steps please mam

[@qASheducationcontent]

Thik hai👍

[@mominjaved7616]

Can u share notes type on my gmail???

[@qASheducationcontent]

Sure, you can drop an email on [e-mail]