Each gene is responsible for a particular protein production in all organisms. So here we insert our gene of own interest into the plasmid (pBR322). That gene can undergo the transcription and translation process and it can produce the protein. In that case if we use the chloramphenicol it restrict the synthesis of protein and the plasmid which carries our gene of own interest can undergo the replication and it won't produce the protein. That's why the copy number can be 1000-3000 per cell. If it produces the protein the copy number is controlled by the rop gene to 40. I Hope it helps you.
It's actually 4361 bp(earlier 4363)...Please refer to Primrose,gene manipulation
It is Stanford University, not california
Thanks sir very nice...
You're welcome
Transgenic plants ka selection kase kre sir ,
Host cell ,konse use kre ge sir , es plasmid k liye
E coli
@@shomusbiologyofficial ok sir thank you
first !
Thank you
❤️
Thank you
Hindi mein samj do n sir
Can you please elaborate @12:30 on protein synthesis blockage by Tetr gene?? Which proteins are you referring to
Each gene is responsible for a particular protein production in all organisms. So here we insert our gene of own interest into the plasmid (pBR322). That gene can undergo the transcription and translation process and it can produce the protein. In that case if we use the chloramphenicol it restrict the synthesis of protein and the plasmid which carries our gene of own interest can undergo the replication and it won't produce the protein. That's why the copy number can be 1000-3000 per cell. If it produces the protein the copy number is controlled by the rop gene to 40.
I Hope it helps you.
Sir agr hindi vedio aati eski tho jyada acche se smjh aata
Nice sir 👍
Thank you
Don't ask me again anas about this topic..
So, look carefully..
Okay Sir👍