Very nicely experience ma'am. Just few months back I succeed your channel and now I'm really enjoying it and learning new things watch each coming day. Thanks ma'am 😊 🙏🏻
Moreover, Can we use ice-chilled ctab buffer instead of liquid nitrogen ? After adding eq. Vol of chl. and IAA, we get to centrifuge the sample, kindly mention the rpm and time. Thank you for your time you're providing here.
Don't use chilled CTAB, for CTAB to be activated u need to preheat the extraction buffer @65.C for 15 min. So if u don't have liquid nitrogen u can directly add the pre heated extraction buffer to ur sample. May I ask what sample are u using?
Make a video on dna extraction from whole blood or plasma. Secondly, i want to know the full recipe of ctab extraction buffer considering i am short with pvp. That would be an honour. Thank you for your time you are providing here.
Well we don't use much amount of PVP but if u are short of it u can treat the sample with chloroform and isoamyl alcohol two times and carefully separate the uppermost layer
So even for seed will we follow the same method? Actually dna isolation is not a routine work in our lab so i was looking for a method for plant dna isolation for quite sometime now.
For seed also you need to crush open the seed for isolating a DNA... The procedure is basically same. Just take appropriate precautions at each step. All the best
Since the cell wall of bacteria is not as complicated as plant u can easily use some chemical, or enzymatic methods for its lysis but because plants have much complicated cell wall different chemicals are used along with mechanical methods and certain chemicals are used to neutralize the metabolites released during crushing of plant tissue. So the chemical utilization is different in both otherwise the basic concept is very similar
This is the basic procedure, u can use it... But recently other methods have been developed which are more significant and improves the quality of genome sequencing
To make 500ml of 5M NaCl solution use the formula--> gm of NaCl = (mol. Wt of NaCl * Molarity* vol. To make) /1000 =( 58.44*5M *500ml) /1000 = 146.1gm Dissolve 146.1 gm NaCl in 500ml D/w
Thanks you very much it's a very beneficial video. Thanks from Palestine 🇵🇸
Very nicely experience ma'am. Just few months back I succeed your channel and now I'm really enjoying it and learning new things watch each coming day. Thanks ma'am 😊 🙏🏻
Your comment just made my day😊
Very nicely explained each and every concept related to this topic 🙂
Brilliant, totally got this experiment from this video rather to tangle in searching and reading.
well explained sis , thank you
WOW...BEAUTIFULLY EXPLAINED THankyou
Very effective explanation mam
More informative Mam..... Thank u 👏
Loved this Manisha
Mam thank you so much really it's was very deep explaination with all over all details keep gng to make useful videos for us ❤️
😊
unforgettable lecture for researcher
Ma'am can you please make a video on different basic calculations like conversion of mg/ L to mM?
Mam, can u pls tell from where the content is taken.. i.e. the reference ??
Mam is this method CTAB used for DNA extraction from plant materials or only for plant cells. I am confused. 😑
Well you can use it for other plant materials too
Good explanation
Moreover, Can we use ice-chilled ctab buffer instead of liquid nitrogen ?
After adding eq. Vol of chl. and IAA, we get to centrifuge the sample, kindly mention the rpm and time.
Thank you for your time you're providing here.
Don't use chilled CTAB, for CTAB to be activated u need to preheat the extraction buffer @65.C for 15 min. So if u don't have liquid nitrogen u can directly add the pre heated extraction buffer to ur sample. May I ask what sample are u using?
U can do the Centrifuge@13000rpm for 1-2 min.
@@manishamalhotra1612 Well i am using different plant tissue samples of leaves, approx. 1cm.
Is it necessary to add pvp for dna extraction from rice?
Make a video on dna extraction from whole blood or plasma. Secondly, i want to know the full recipe of ctab extraction buffer considering i am short with pvp. That would be an honour.
Thank you for your time you are providing here.
Well we don't use much amount of PVP but if u are short of it u can treat the sample with chloroform and isoamyl alcohol two times and carefully separate the uppermost layer
I am considering your points. Now I would look forwad for full Ctab buffer recipe from you.
Mail me at manishamalhotra92@gmail.com
EXCELLENT LECTURE
So even for seed will we follow the same method? Actually dna isolation is not a routine work in our lab so i was looking for a method for plant dna isolation for quite sometime now.
For seed also you need to crush open the seed for isolating a DNA... The procedure is basically same. Just take appropriate precautions at each step. All the best
Thankuu mam for this nyc explanation
What is main difference in isolation of DNA from bacterial cells and from plant m cnfuse dua to lot of chemicals and steps
Since the cell wall of bacteria is not as complicated as plant u can easily use some chemical, or enzymatic methods for its lysis but because plants have much complicated cell wall different chemicals are used along with mechanical methods and certain chemicals are used to neutralize the metabolites released during crushing of plant tissue. So the chemical utilization is different in both otherwise the basic concept is very similar
👍👍
Thank you for a very detailed explanation. Helps a lot!
Is this good for cpDNA extraction if I would like to sequence the complete chloroplast genome?
This is the basic procedure, u can use it... But recently other methods have been developed which are more significant and improves the quality of genome sequencing
You can go through a paper published in nature on a protocol regarding this by Dan Liu
Thank you ma'am❤️
how to dissolve 5M NaCl in 500ml dwater
To make 500ml of 5M NaCl solution use the formula--> gm of NaCl = (mol. Wt of NaCl * Molarity* vol. To make) /1000
=( 58.44*5M *500ml) /1000 = 146.1gm
Dissolve 146.1 gm NaCl in 500ml D/w
Thank you very muctch
thanks
Can you explain in hindi plese
thanksssssssssssssssssssssssssssssssssssssssssssssssssssssssssssss
Thanks