really simple but instructive video, thank you. I wish there was a step-by-step on how you can sterilize your environment and check if everything is ready for work
Love these videos! Super informative! A little tip I’ve seen done before is mixing activated charcoal with your agar plates so you can see growth with easier contrast as well as its slight antiseptic effect! Don’t stop doing what you do!
I had some spore prints that were contaminated with bacteria or yeast. Every section of my streaks were just a cream colored liquid after a few days and no mycelium grew. I was sad because it was from some really choice mushrooms. My problem was that I didn't put them into a still air box to get the spore print and I placed the foil in a book instead of a sanitized plastic ziploc bag 😔 The learning curve can be quite a battle in the beginning
@@ScottWConvid19 If you didn’t use damp rid crystals I would blame it on that too. If the spore print is wet it will surly contaminate. Here’s the video about taking a clean/dry spore print: th-cam.com/video/URHWiiZDwmU/w-d-xo.html
@@SporenSprout so if you divided the whole thing into 10 plates they'd potentially all have visible variation from each other? How do you know you're getting the genetics that you want?
@ Yes exactly, they will show different phenotypes. One culture might produce tall skinny fruits and one might produce short thick fruits. If you get a perfect fruit during harvest you can take a tissue clone from it and then you will have narrowed it down to exactly 1 strain and the mushrooms should be more consistent in similarity.
@@SporenSprout OOH. So you're saying that the spores might have differences, but then you're selecting out the best ones the next time and doing a different method of propagation. That clears it up immensely. Thank you!
best videos on youtube have no music like this
really simple but instructive video, thank you. I wish there was a step-by-step on how you can sterilize your environment and check if everything is ready for work
Love these videos! Super informative!
A little tip I’ve seen done before is mixing activated charcoal with your agar plates so you can see growth with easier contrast as well as its slight antiseptic effect!
Don’t stop doing what you do!
Do have anything in the agarplate or is it completely clean? I mean some „food“ for the mycelium
My Favorite Recipe for Making Agar Plates Full Tutorial
th-cam.com/video/otBU3jlUZAE/w-d-xo.html
Thanks for sharing
@@landerson2873 Thanks for watching!
Thanks ❤
Great videos!
@@brianhicks4730 Thanks for watching!
I had some spore prints that were contaminated with bacteria or yeast. Every section of my streaks were just a cream colored liquid after a few days and no mycelium grew. I was sad because it was from some really choice mushrooms. My problem was that I didn't put them into a still air box to get the spore print and I placed the foil in a book instead of a sanitized plastic ziploc bag 😔
The learning curve can be quite a battle in the beginning
@@ScottWConvid19 If you didn’t use damp rid crystals I would blame it on that too. If the spore print is wet it will surly contaminate. Here’s the video about taking a clean/dry spore print:
th-cam.com/video/URHWiiZDwmU/w-d-xo.html
@@SporenSprout Well that's something I've never heard of before. Thanks I'll check it out! 👍🏼👍🏼
I think I missed something. Do you only transfer a bit from one plate to the other just to multiply them? Or is there another reason?
@@Malprincess I transfer a small piece so the genetics are narrower.
@@SporenSprout so if you divided the whole thing into 10 plates they'd potentially all have visible variation from each other? How do you know you're getting the genetics that you want?
@ Yes exactly, they will show different phenotypes. One culture might produce tall skinny fruits and one might produce short thick fruits. If you get a perfect fruit during harvest you can take a tissue clone from it and then you will have narrowed it down to exactly 1 strain and the mushrooms should be more consistent in similarity.
@@SporenSprout OOH. So you're saying that the spores might have differences, but then you're selecting out the best ones the next time and doing a different method of propagation. That clears it up immensely.
Thank you!
what kind of flow hood do you use?
Cool
Thanks for the great video ❤
@@MuhammedIbraheem-qr9go Thanks for watching!
1:50 How to Pre-Hydrate spores ?
@@SomeOneHigh Don’t need to but you could add the spores to sterile water and make spore syringes first
What incubator did you use?
@@barbarossa3976 I don’t use an incubator, just a warm room on a shelf.