DNA Sequencing | Sanger or chain Termination Vs automated DNA sequencing | Video 5

Sanger sequencing, also known as the “chain termination method”, is a method for determining the nucleotide sequence of DNA.
Sanger sequencing can be performed manually or, more commonly, in an automated fashion via sequencing machine.
Sanger Sequencing
Steps 1. DNA Sequence For Chain Termination PCR
2. Size Separation by Gel Electrophoresis
3. Gel Analysis & Determination of DNA Sequence For detailed information watch the video
#CapillarySequencing #SangerMethodOfSequencing

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Umair Farooq From Sargodha
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I'm a 10th class student and we chose DNA sequencing as our topic for biology exhibition. Wanted to get knowledge on the topic, very nicely understood. Thank you very much 🙏🏻

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DNA sequencing and 10th class?
We're studying it in 12 th class lol!

[@bloom_official_]

​@@manahil558 Yeah ik, it's just we wanted to choose a unique topic of higher grade which can be understood by 10th class students, so we chose this, and it went very well! We also made a model of the gel electrophoresis and a presentation of the process to explain ^^

[@manahil558]

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Sir when sequence of DNA is not known then how we will know that which sequence of primer we have to use

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Lkn is me jo hm ny sequence find out krna wo ap ny phly likh lia
Agr na likhen
New kren
To hm kasy kren ge wo smjh ni ae
Coz ap to us dye hoe sequence ki help sy krr rhy jo find out krna

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Sir es ma konsa radioactive substance used huwa ha ???

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Poly acrylamide gel we use for protein separation but for DNA or RNA we use agaros gel .so how do you say that we use in sangar sequencing polyacrylamid gel in this method please explain ..?

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