DNA Sequencing | Sanger or chain Termination Vs automated DNA sequencing | Video 5
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- เผยแพร่เมื่อ 29 ส.ค. 2024
- Sanger sequencing, also known as the “chain termination method”, is a method for determining the nucleotide sequence of DNA.
Sanger sequencing can be performed manually or, more commonly, in an automated fashion via sequencing machine.
Sanger Sequencing
Steps 1. DNA Sequence For Chain Termination PCR
2. Size Separation by Gel Electrophoresis
3. Gel Analysis & Determination of DNA Sequence For detailed information watch the video
#CapillarySequencing #SangerMethodOfSequencing
THANK YOU SIR YOU ARE A SAVIOR FROM 11TH TILL NOW MAY ALLAH GRANT YOU MORE SUCCESS🤲🤲
Pak se ho bhai
Clear my concept in short video may Allah bless you more and more
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Umair Farooq From Sargodha
Govt. AMC college Sargodha
Thank you so much for this valuable lecture...kal ka mera test ab Insha Allah bohot acha hoga ..... jazakallah khair 😊
I'm a 10th class student and we chose DNA sequencing as our topic for biology exhibition. Wanted to get knowledge on the topic, very nicely understood. Thank you very much 🙏🏻
DNA sequencing and 10th class?
We're studying it in 12 th class lol!
@@manahil558 Yeah ik, it's just we wanted to choose a unique topic of higher grade which can be understood by 10th class students, so we chose this, and it went very well! We also made a model of the gel electrophoresis and a presentation of the process to explain ^^
@@bloom_official_ Interesting! You must be blessed to study in this type of school. Demonstrations are the most amazing part!
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Sir when sequence of DNA is not known then how we will know that which sequence of primer we have to use
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Yve not completed all the queries being arises during watching you
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thnku so much❤💯
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Thank u sir
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Acha padhate ho sir jay shree Ram
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Thanks sir
Fragments of DNA mein nitrogenous bases ki arrangements ko find out kr skty or agr ap Ksi DNA KY fragments ki DNA sequencing ko maloom krlty tu uski bht sari. Application hosktu DNA ki mutation find out kerny k lye use kerskry genetic disorder find out krnt KY lye use kerskry evoultio ary study. Lye use keakryp
Thanks alot
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2nd method??😢
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Well explained!✨
sir plz FISHER'S VIEW and ZAHAVI'S HANDCAP THEORY. EVOLUTION AND PRINCIPLES OF SYSTEMATICS .topic pa video bna da humble request ha plz sir.
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Hi sir please make video on next generation sequencing method
Thanks sir
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Sir please upload lecture on Microarray technology (DNA microarray )
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Baqi to sb smjh aa gae sir
Lkn is me jo hm ny sequence find out krna wo ap ny phly likh lia
Agr na likhen
New kren
To hm kasy kren ge wo smjh ni ae
Coz ap to us dye hoe sequence ki help sy krr rhy jo find out krna
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Masha ALLAH
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Mashallah
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Maxam gilbert sequencing bhi bnayiye sir
Slm sir
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Sir es ma konsa radioactive substance used huwa ha ???
Bhaii plzzz RFLP and RADP pr video bna dy 🙏🏻
Poly acrylamide gel we use for protein separation but for DNA or RNA we use agaros gel .so how do you say that we use in sangar sequencing polyacrylamid gel in this method please explain ..?
Apny bhi wo nhi daykha jo bohat acha smjaya balky coman logon ki trha galti daykhi 😮
Ram ram sir🙏🙏
Thank You Thank You Sirrr!!!!❤️❤️❤️