Steps of Dna Fingerprinting:- 1) Extracting Genome(whole DNA) from cells. 2)Optional Step:-if amount of DNA obtained is very less then DNA is amplified by PCR. 3)Use Of Restriction Enzyme to cut DNA into fragments in the end we will be able to observe RFLP(Restriction fragment length Polymorphism) in Mini Satelite region due to VNTR, Size of VNTR varies from person to person 99.9 DNA of all human is same 0.1 percent is different due to VNTR and that 0.1 percent is enough to to tell person apart unless they are monozygotic twins. 4) Gel Electrophoresis, here DNA fragments are seperated on the basis of their Length/Charge higher the Length/(-ve) Charge the closer it is to Cathode. 5) Denaturation In alkaline Medium, here double stranded DNA is denatured into Single Stranded DNA. High Temperature is not used in Denaturation since there is risk to mely the gel. 6) Southern Blotting, DNA is transferred to Nylon/Nitrocellulose Membrane, Gel is removed. 7) Hybridization, In this step Radioactive VNTR probe prepared in lab Complimentary to VNTR in DNA is transferred to Nylon membrane Since DNA was single stranded Radioactive VNTR probe gets attached to VNTR. 8)Auto Radiography, X-ray Film is placed on Nylon membrane Radiations from where Radioactive VNTR probe was attached is now Stike X-ray Film and hence a pattern is observed. Pattern is Different in person due to size of fragment of VNTR. Uses:- 1) Forensic Science. 2) Determining Population and Genetic Diversity. 3) Paternity test(The RFLP of child is 50% match with mother and 50% with father because child gets its 23 chromosome from mother and 23 from father) Fun Fact:- Since mature RBCs don't have nucleus WBCs are used in DNA Profiling
This explanation conflates two different techniques. The old method of DNA fingerprinting used restriction enzymes. The new method uses PCR. The old method requires a lot of DNA, the new method does not. Once an individual's STR's are amplified enough to see on a gel, you run the DNA directly - no cutting it up or you will skew your results! If you look at enough STR's (~13) the probability that two individuals would have all 13 STR's be the same length is small enough to distinguish among 7 billion people. You guys put out really good stuff, but I can't share this with my students as it is! Would love to see another version, because your graphics and pacing are really good.
thank you so much! I have been studying this for half an hour and i still had not understood. Thanks to this amazing and simple explanation I finally got it.
I like this video. It’s not a boring lecture but a fun educational video. I found myself not getting bored with it. And I love how you did the examples at the end to have people try it out for themselves.
My lecturer always said it's not a scientist's job to say 'suspect is guilty'. A scientist just provides evidence. It would be better if the video said 'suspects 3 DNA sample matched the same at the crime scene'. If you're looking to do forensics at university. Remember this.
Thank you very much for making this video. It became crystal clear to me after seeing this video. I was really searching for this kind of video. Thank you very much
I wanted to ask three questions to do with this video. My first question is Explain what an STR is. My second question is Explain what a genetic locus is in the context of DNA profiling. My third question is Explain briefly why STRs can be used to identify individuals in DNA profiling. Please respond to my comment ASAP today.
@@fuseschool I wanted to ask three questions to do with this video. My first question is Explain what an STR is. My second question is Explain what a genetic locus is in the context of DNA profiling. My third question is Explain briefly why STRs can be used to identify individuals in DNA profiling. Please respond to my comment ASAP today.
@Hafsah Yaser, STRs mean Short Tandem Repeats. In forensic labs, they are normally used to create a genetic fingerprint of an individual. When is comes to DNA profiling, - it tells scientists if the DNA is from a man or woman, for example, and if the sample being tested belongs to a particular person. When scientists produce DNA profile, they examine STRs at 10 or more genetic loci, which are usually on different chromosomes.
Thank you so so much can't thank you enough . I was tired of attending LECTURES on fingerprinting where they make you write an Essay on this and still nothing goes inside your head as there is no way of Imagining it
Exactly what I was looking for. No dry lecture, just a short, perky little video - thanks.
Glad we could help!
Yes, I’m doing a profiling assignment right now and was a little confused on base pairs so I looked this up and it helped a lot!
same
Same here..it is very helpful!!!
Textbooks(NCERT) lines were boring and tough to understand but you have made them easy with this video... Thank you very much
Steps of Dna Fingerprinting:-
1) Extracting Genome(whole DNA) from cells.
2)Optional Step:-if amount of DNA obtained is very less then DNA is amplified by PCR.
3)Use Of Restriction Enzyme to cut DNA into fragments in the end we will be able to observe RFLP(Restriction fragment length Polymorphism) in Mini Satelite region due to VNTR, Size of VNTR varies from person to person 99.9 DNA of all human is same 0.1 percent is different due to VNTR and that 0.1 percent is enough to to tell person apart unless they are monozygotic twins.
4) Gel Electrophoresis, here DNA fragments are seperated on the basis of their Length/Charge higher the Length/(-ve) Charge the closer it is to Cathode.
5) Denaturation In alkaline Medium, here double stranded DNA is denatured into Single Stranded DNA. High Temperature is not used in Denaturation since there is risk to mely the gel.
6) Southern Blotting, DNA is transferred to Nylon/Nitrocellulose Membrane, Gel is removed.
7) Hybridization, In this step Radioactive VNTR probe prepared in lab Complimentary to VNTR in DNA is transferred to Nylon membrane Since DNA was single stranded Radioactive VNTR probe gets attached to VNTR.
8)Auto Radiography, X-ray Film is placed on Nylon membrane Radiations from where Radioactive VNTR probe was attached is now Stike X-ray Film and hence a pattern is observed. Pattern is Different in person due to size of fragment of VNTR.
Uses:-
1) Forensic Science.
2) Determining Population and Genetic Diversity.
3) Paternity test(The RFLP of child is 50% match with mother and 50% with father because child gets its 23 chromosome from mother and 23 from father)
Fun Fact:-
Since mature RBCs don't have nucleus WBCs are used in DNA Profiling
I was looking for a proper video of DNA test . And found here one.Thanks a bunch . Love from Bangladesh
Welcome! Glad you liked it 🙂
hope u go to jannat with 72 hoors
This explanation conflates two different techniques. The old method of DNA fingerprinting used restriction enzymes. The new method uses PCR. The old method requires a lot of DNA, the new method does not. Once an individual's STR's are amplified enough to see on a gel, you run the DNA directly - no cutting it up or you will skew your results! If you look at enough STR's (~13) the probability that two individuals would have all 13 STR's be the same length is small enough to distinguish among 7 billion people. You guys put out really good stuff, but I can't share this with my students as it is! Would love to see another version, because your graphics and pacing are really good.
i love you so much
I've huge respect for teachers like you 😊
Thank you. Right now I have an assignment to find more info about RFLP in DNA fingerprinting.
PCR is different, it produces many copies of required DNA in a test tube
@@HayDayEveryday I have a doubt regarding pcr .. why do we have to generate billions of same copies? Isn't 1 dna sample enough?
Fuse school is the best educational channel .. hope u r not running in a loss for putting this much of ur effort and animations
Thank you so much for your kind words 💜
After reading in my book, I thought it's so much difficult. Now it's very much easy after watching this video. Thank you so much for this video 😊😊😊
Most welcome 😊 Glad it helped!
I allready Knowles everything about the video
Crisp yet lucid explanation! Thank you for this one!👍👍
Exactly what i need for a quick revision a night before the exam.
Thanks a ton! ❤
Happy to help! Hope it went well.
thank you so much! I have been studying this for half an hour and i still had not understood. Thanks to this amazing and simple explanation I finally got it.
You are so welcome! Glad it helped!
Really such a great video !! All concepts explained so nicely !! Thank you !!!!
You are most welcome! Glad you liked it!
I can't thank you enough, I have my exam in a week, and I will score good because of you guys, thanks a lot
Lovely to hear! All the best for your exam.
Easy peasy with no confusion, thank you.
Lots of love from India🙏
You are very welcome!!
Thank you so much! It has helped greatly because I have to answer some questions on this
That was extremely helpful, thank you so much !
I like this video. It’s not a boring lecture but a fun educational video. I found myself not getting bored with it. And I love how you did the examples at the end to have people try it out for themselves.
Thank you so much! Glad you liked it!
not the rick roll🧍♀
The video is Soo good that no comments are needed , everything is according to ncert.. Thank you for this amazing video
Thanks for your feedback! Much appreciated!
Got biology exam tomorrow so watching this today 😭🙏🏼
That's an amazing short video on DNA finger printing. Thanks a lot for this kind of video. Keep making this videos. It will be good for all of us.
Thank you! Glad you liked it!
Thank-you sir DNA is my favorite concept.
Thanks for watching!
Even though i'm korean, i can understand VNTR easily thanks to your examples. Thank you for GOOD video
Happy to hear that! 😊
Are you BTS ARMY?:😁
Fuse school always helps me to take my understanding of the topics while studying to the next level.
Glad we can help 🙂
The video was exactly what i wanted for my exam and it was realy intressing ,so therefore i subscribed this video.
If you likes this video then like my cement, Please.
Glad you liked the video!
Really Superb. Simply explained in a short way.
Glad you liked it!
My lecturer always said it's not a scientist's job to say 'suspect is guilty'. A scientist just provides evidence. It would be better if the video said 'suspects 3 DNA sample matched the same at the crime scene'. If you're looking to do forensics at university. Remember this.
short and sweet with good examples and making it crystal clear. love from India.
Thanks! Glad you liked it!
@@fuseschool 😀😀😀😀😀
Thank you very much for making this video. It became crystal clear to me after seeing this video. I was really searching for this kind of video. Thank you very much
You're very welcome!
JUST WOWWWWW.....😊THIS IS SOOO AMAZING...ALL CONCEPTS CLEARED IN 5 MINUTES👌
🤗❤️
super video! Loved the simple tasks in the end :)
wow u have saved 1 hour , best video
ThankYou so much dearest sir.
May you live long!
You are most welcome!
Hi
Deep explanation in very short video .. thanks a lot 🥰sir ... sending love from India
You are most welcome! 😃
Where r u from sir ?
Thanku so much sir.
Now this topic is clear to me.
Thank u so much.... And this was the type of explanation I was looking for.
Most welcome! Glad it helped!
Thank you so much sir, it was so helpful 😊
We happy to hear you found the video helpful. Hope you will find more videos that you can check out!
Thank you so much. The video is nice and it clarifies my concept easily.
Glad it was helpful!
@@fuseschool yes it was very helpful
This made more sense than any other
Great!
In just 4 minutes u just explained the most difficult topic that's what I was looking for thanks.
You're welcome 😊
Amazing video! Just what I wanted!
Great! Glad you liked it!
When he said Loci.. The first thing that came to my mind was Loki
Happens to the best of us
Me too
Mee too
😂😂😂
This is What I was looking for 🙏❤️
Fantastic 😃
Thanks a lot now DNA fingerprint is crystal clear to me
Glad to hear it!
Hiii dear I saw u
U was my classmate
Thanks man, you are a lifesaver
Very nicely explained
Very well explained !! TQ Fuse School 👍🏻👍🏻 .. helps me as I share this in my tuition group.
Thank you....this what I wanted visualize.
Perfect! Glad you found it 🙂
Thank you so much. All concept in just 4 minutes.😍
Saved time with complete knowledge
Most welcome 😊
This was a very beautiful video, thank you so much 👌👌
Thank you! Glad you enjoyed it!
This is awesome. Thanks for the video
No problem 👍
Short video with great explanation you helped me thanks I am from somalia🇸🇴🇸🇴
Glad to hear that! Glad it helped!
Short but more than I expected 😊
😉
Thanks a lot for this video
This video is very helpful for my exam to understand dna fingerprinting.
Glad it was helpful!
the last part was fun !
🙂
Excellent explaination.... Keep making such helpful videos ☺️☺️👍👍
Nice to hear! Thank you!
Thank you for the explaination.This vid really help me
Happy to hear that! Glad it helped!
This is exactly what I need ...
Thank you so much 🙏
You're so welcome 😊
Thank you so much! I couldn't understand ncert lines properly, it really helped
Glad it helped!
I wanted to ask three questions to do with this video. My first question is Explain what an STR is. My second question is Explain what a genetic locus is in the context of DNA profiling. My third question is Explain briefly why STRs can be used to identify individuals in DNA profiling. Please respond to my comment ASAP today.
@@fuseschool I wanted to ask three questions to do with this video. My first question is Explain what an STR is. My second question is Explain what a genetic locus is in the context of DNA profiling. My third question is Explain briefly why STRs can be used to identify individuals in DNA profiling. Please respond to my comment ASAP today.
@Hafsah Yaser, STRs mean Short Tandem Repeats. In forensic labs, they are normally used to create a genetic fingerprint of an individual.
When is comes to DNA profiling, - it tells scientists if the DNA is from a man or woman, for example, and if the sample being tested belongs to a particular person. When scientists produce DNA profile, they examine STRs at 10 or more genetic loci, which are usually on different chromosomes.
best explanation. Short and precise ! good job
Thank you! Much appreciated!
Great video. Loved it
Thank you!
wowwww what a explanation amazing
Hope you found the video helpful. Check out and comment on more videos.
That's great ,, this is so helpful for me....
Thanks a lot🤗🤗
Great! Glad it was helpful!
Amazing video for DNA test
Great explanation!
Thanks!
Thank you sooo much sir it's really outstanding lecture💜
Most welcome! Glad you liked it! 💜
Awesome 🧬💉🧫
Thank man you are a great and humble for us❣️our support is with you..💯
We appreciate that!
Very helpfull thanku😊
Glad you found the video helpful. Thank you for watching
Best explanation thanx a lot..😊
Awesome! Thanks!
Thanks 👍 that's what I am looking for 😊 now i understand the exact meaning of DNA fingerprinting💗
You're welcome 😊
Very simple and comprehensive
Thank you!
Nitrogen bases are not repeated correctly in loci, I think I am not sure about that but go through
Thanks for your presentation 🙂
Amazing explanation
Thank yous so much!
Neet h 12 ko😂
thank you
Wonderful explaination 🙏🙏
Thank you!
Really good 👍👍
Thank you. It was really helpful.
Glad it was helpful!
Nice explanation
Thank you!
Very helpful video ....I want exact this video.....thank you....😊😊😊😊😊😊😊
Most welcome 😊
Very good lecture
MY TEACHER SENT ME HERE
the add was cool ngl
Amazing!
Thanks!
Wow what an interesting topic I love learning about DNA it so interesting thanks for this video 😏😏😏😏😉😉😉👍🏻👍🏻👍🏻❤️❤️❤️
Our pleasure 😊 Glad you enjoyed it!
Amaaaaazing
Tnx for making it interesting
Thanks for watching 🤗
Thank you so much 😇.
Always welcome 😊
Excellent Sir.
Many thanks!
Very much nice
With oral nd 3d explanation
Very thanks you sir
You helped me in my exam
You are very welcome!
amazing please make more videos of this and also add some beautiful satisfiying colours please i want to learn more
We'll try 😉
Thank you....sooomuch it really helped me a lot
So glad to hear that it helped! Most welcome 💜
Thank you so so much can't thank you enough . I was tired of attending LECTURES on fingerprinting where they make you write an Essay on this and still nothing goes inside your head as there is no way of Imagining it
You are most welcome! Happy to help!
OMG WHAT A EXPLANATION ✌
Thanks a lot! It helped a lot!
You're welcome! Glad it helped!
Neet ki bachhi👍
Very nice and easy explanation thank you so much for this video.... 👍
Glad you liked it!
How was that probe made ? from where you get the sequence for making probe??? please explain
what probe did you take during southern hybridisation ???? as we need probe during southern hybridisation
This might help: www.nature.com/wls/definition/southern-blot-289/
Nice video dude ☺️
Thanks 😁
Good explanation but probe hybridization step is not mentioned here
Jeffrey gave this technique in 1984....this year is given by my book...
Awesome👍👍👏👏👏
Thanks 🤗