From what I understand, the main reason human stem cells are used are because of the factors secreted by the stem cells. So whereas unconditioned stem cells can differentiate into various new cells or replace damaged cells, cultured or conditioned stem cells apparently just give the correct signals from growth factors to signal your own existing cells to heal themselves. The main issue I see with unconditioned stem cells is whether they are actually able to differentiate into new cells because stem cells usually require specific environments, such as internal body environments to exhibit their full potential. Either way I think uncultured/unconditioned stem cells are better but I don’t know if there’s enough data to show whether human stem cell and fibroblast conditioned media are as good or better when it comes to growth factors.
That depends on what condition we are dealing with, and a person's body weight, age and severity of health issues... Generally at our clinic we use between 20 to 60 million unexpanded (native cells) which are more potent and with less potential for side effects than expanded cells. To learn a little bit more about how the cell therapy are conducted, please watch this video: Best Place to Get Stem Cell Therapy and Treatment Approaches th-cam.com/video/VpICLGyVMfY/w-d-xo.htmlsi=2nkpMgyJc-iO09lf
Generally by passage 4 cells begin to degrade. It is not about "how many times" but "how many passages" the cells are expanded as I explained in the video.
It would be very helpful, and less confusing to the patient looking for a treatment with umbilical cord derived mesenchymal stem cells (uMSC) if the scientists, medical practitioners, and clinics could come to some "common ground" with regard to dosage, application (IV vs IM), and source. Additionally it would help if there was a common perspective on the viability of these cells in the body after IV or IM. I have read and heard a variety of answers with regard to maximum detectable in the blood stream. A Phd mentioned that the host immune system eliminates the introduced cells in four hours; therefore exosomes are superior. In my research, both videos and research reports, the maximum time that uMSCs are detectable in the bloodstream varied from three days, seven days via IV, and twenty-nine days via IM. Many clinics outside the US use expanded uMSCs more research is needed in this field to develop a whole perspective.
You are absolutely right about more research needed in the entire stem cell arena, and the question of cell expansion is an important one, and we will have more definitive answers as time goes on. Sometimes consensus may not be available because in new areas of science, it taken TIME for new research and evidence to fully emerge. But when given time, the picture may become more clear. That said, I do it agree with the PhD's opinion. There has been clear research showing that cells survive in the recipient's body, readily detectable even in 100 days. Anyhow, we are all on this path of discovery, but a trusted a source of information is helpful - from people who have read the science and research...
Thank you Dr.for the response, unfortunately I watched another video on exosomes by the PhD and he said 1 month persist in the body, as told to me by Springs Rejuvenation exosomes can persist in the body for 2 to 4 months. Where is the data ? same thing about umbilical cord MSCs where is the definitive data ? Thanks again for responding to my comment @@JoyKongMD
"Native" means that cells have not been manipulated in any way, such as through the use of chemicals or drugs or via the expansion process (growing the sauce to a large number in a culture medium in an incubator). Any cells that have not been manipulated in those ways are considered "native." Autologous cells means the cells came from the same person.
Hi doctor. Do these non cultured cells differentiate into nerve cells if applied intrathecally? What are the benefits for such system of administration?
It is difficult to say whether it will or will not. Studies have definitely shown that MSCs have the potential to differentiate into neurons, but the questions of to what extent and whether it could always happen are not answered yet... Systemic administration will allow cells to communicate with a person's immune system which will have profound effects on the healing of the entire body... also, IV administered MSCs can cross the blood brain barrier to some extent, thus can exert benefits locally as well...
Evidence suggest that within 3 days of intravenous infusion of stem cells, most of the cells would have migrated out of the lungs, especially if there is no lung pathology which would tend to attract the cells to stay.
Many studies have used various number of cells. Most studies use 1 million cells per kg. However, these studies use expanded cells which generally have less potency than native (unexpanded) cells. Most doctors, when using native cells, give people in the range of 3 million to 60 million cells through IV route, depending on what kind of product and cells (whether it is cord blood cells or MSCs) are used.
I been to DBC In puerto vahartta last month and I feel great from it so cell replicate is way to go especially 9-12 months baby also the clinic price is affordable. Why should anyone pay 5000 thousand dollar from bone marrow from a 30 years old person with less stem cell than pay 3000 for 100 million cell.
Glad you feel great after stem cell therapy! Cell replication to certain degree may be fine, esp for MSCs, but above 3 passages there tend to be issues such as inflammatory reactions or immune rejection issues...
Not really it varies to people but the cells at DBC been scan and screen for virus or any disease three times by different FDA in Mexico and the donor are question medical history for how many sex partner, bacteria, cancer and has to be age 18-26. I was also presented a certificate of screening for cells I was given where it came from before the treatment so above am beyond standard for safety. I still feel great after the treatment so no rejection from myself or other individual that went to DBC.
That's great that you were getting good response. All the certificates as far as sterility and screening etc are quite standard, so I'm glad you got those. Glad no rejection issues were noted.
@CTCt_7501TH listening are everything they say is true maybe but I gotten good result no back pain my brain fog is gone I can think more clearly and my energy through the roof. I done some research and digging before I chose DBC so as far as price goes DBC wins over the USA, CPI and Bioxcellorator in Colombia.
From what I understand, the main reason human stem cells are used are because of the factors secreted by the stem cells. So whereas unconditioned stem cells can differentiate into various new cells or replace damaged cells, cultured or conditioned stem cells apparently just give the correct signals from growth factors to signal your own existing cells to heal themselves. The main issue I see with unconditioned stem cells is whether they are actually able to differentiate into new cells because stem cells usually require specific environments, such as internal body environments to exhibit their full potential. Either way I think uncultured/unconditioned stem cells are better but I don’t know if there’s enough data to show whether human stem cell and fibroblast conditioned media are as good or better when it comes to growth factors.
Thanks 🙏🏾 for clearing that up Dr Kong!!
You are very welcome!
Many thanks for sharing!!!
Thank you for your kind remark.
THANKYOU!!!!
You are welcome!
At one time in stem cell therapy treatment how many numbers of stem cells injecting?
That depends on what condition we are dealing with, and a person's body weight, age and severity of health issues... Generally at our clinic we use between 20 to 60 million unexpanded (native cells) which are more potent and with less potential for side effects than expanded cells. To learn a little bit more about how the cell therapy are conducted, please watch this video:
Best Place to Get Stem Cell Therapy and Treatment Approaches
th-cam.com/video/VpICLGyVMfY/w-d-xo.htmlsi=2nkpMgyJc-iO09lf
Love it🔥👏
Thank you!❤️
@@JoyKongMD Certainly❤
20:42 so an initial expansion of ten times sounds ok then. How many passes would that be equivalent to?
Generally by passage 4 cells begin to degrade. It is not about "how many times" but "how many passages" the cells are expanded as I explained in the video.
It would be very helpful, and less confusing to the patient looking for a treatment with umbilical cord derived mesenchymal stem cells (uMSC) if the scientists, medical practitioners, and clinics could come to some "common ground" with regard to dosage, application (IV vs IM), and source.
Additionally it would help if there was a common perspective on the viability of these cells in the body after IV or IM. I have read and heard a variety of answers with regard to maximum detectable in the blood stream. A Phd mentioned that the host immune system eliminates the introduced cells in four hours; therefore exosomes are superior. In my research, both videos and research reports, the maximum time that uMSCs are detectable in the bloodstream varied from three days, seven days via IV, and twenty-nine days via IM.
Many clinics outside the US use expanded uMSCs more research is needed in this field to develop a whole perspective.
You are absolutely right about more research needed in the entire stem cell arena, and the question of cell expansion is an important one, and we will have more definitive answers as time goes on. Sometimes consensus may not be available because in new areas of science, it taken TIME for new research and evidence to fully emerge. But when given time, the picture may become more clear.
That said, I do it agree with the PhD's opinion. There has been clear research showing that cells survive in the recipient's body, readily detectable even in 100 days. Anyhow, we are all on this path of discovery, but a trusted a source of information is helpful - from people who have read the science and research...
Thank you Dr.for the response, unfortunately I watched another video on exosomes by the PhD and he said 1 month persist in the body, as told to me by Springs Rejuvenation exosomes can persist in the body for 2 to 4 months. Where is the data ? same thing about umbilical cord MSCs where is the definitive data ? Thanks again for responding to my comment @@JoyKongMD
So by "native" are you referring to Amniotic MSCs? Or you are referring to MSCs that haven't been expanded? Or by native do you mean "autologous"?
"Native" means that cells have not been manipulated in any way, such as through the use of chemicals or drugs or via the expansion process (growing the sauce to a large number in a culture medium in an incubator). Any cells that have not been manipulated in those ways are considered "native."
Autologous cells means the cells came from the same person.
Hi doctor. Do these non cultured cells differentiate into nerve cells if applied intrathecally? What are the benefits for such system of administration?
It is difficult to say whether it will or will not. Studies have definitely shown that MSCs have the potential to differentiate into neurons, but the questions of to what extent and whether it could always happen are not answered yet... Systemic administration will allow cells to communicate with a person's immune system which will have profound effects on the healing of the entire body... also, IV administered MSCs can cross the blood brain barrier to some extent, thus can exert benefits locally as well...
@joykongmd thank you doctor.
What are your thoughts on the exosome microneedling treatments that are becoming popular?
Sure, it would be very helpful for skin regeneration.
Can you still precribe bpc 157 for healing and other peptides for sleep and anxiety
Yes
Does this umbilical cord derived stem cells can trap in lungs when giving systemic infusion?
Evidence suggest that within 3 days of intravenous infusion of stem cells, most of the cells would have migrated out of the lungs, especially if there is no lung pathology which would tend to attract the cells to stay.
How many cells would you use in a typical iv
Many studies have used various number of cells. Most studies use 1 million cells per kg. However, these studies use expanded cells which generally have less potency than native (unexpanded) cells. Most doctors, when using native cells, give people in the range of 3 million to 60 million cells through IV route, depending on what kind of product and cells (whether it is cord blood cells or MSCs) are used.
@@JoyKongMD how many do you typically use in your clinic
I been to DBC In puerto vahartta last month and I feel great from it so cell replicate is way to go especially 9-12 months baby also the clinic price is affordable. Why should anyone pay 5000 thousand dollar from bone marrow from a 30 years old person with less stem cell than pay 3000 for 100 million cell.
Glad you feel great after stem cell therapy! Cell replication to certain degree may be fine, esp for MSCs, but above 3 passages there tend to be issues such as inflammatory reactions or immune rejection issues...
Not really it varies to people but the cells at DBC been scan and screen for virus or any disease three times by different FDA in Mexico and the donor are question medical history for how many sex partner, bacteria, cancer and has to be age 18-26. I was also presented a certificate of screening for cells I was given where it came from before the treatment so above am beyond standard for safety. I still feel great after the treatment so no rejection from myself or other individual that went to DBC.
That's great that you were getting good response. All the certificates as far as sterility and screening etc are quite standard, so I'm glad you got those. Glad no rejection issues were noted.
@CTCt_7501TH listening are everything they say is true maybe but I gotten good result no back pain my brain fog is gone I can think more clearly and my energy through the roof. I done some research and digging before I chose DBC so as far as price goes DBC wins over the USA, CPI and Bioxcellorator in Colombia.