Immunoprecipitation

( www.abnova.com ) - Immunoprecipitation (IP) is the technique of precipitating an protein antigen out of solution using an antibody specific to that antigen. This antibody is immobilized on a solid-phase substrate such as protein A/G agarose beads. The beads are then added to the protein mixture and the proteins that are targeted by the antibodies are captured onto the beads. More videos at Abnova www.abnova.com

[@netaburnum1359]

Co-IP is a powerful technique that is used regularly by molecular biologists to analyze protein-protein interactions. A particular antibody often selects for a subpopulation of its target protein that has the epitope exposed, thus failing to identify any proteins in complexes that hide the epitope.

[@Wlovez]

Really important to consider. so how would circumvent this?