Thank you, this video is perfect! I had training on this yesterday but with so many steps, it's tricky to remember everything. I'm currently studying for a Masters degree in Infectious Diseases and I have to use this technique for my Thesis project. Thanks again :)
@@JESUSSavedMefromDeath I did indeed ☺️ Studying towards the next qualification now. It feels like it never ends but hopefully it pays off in the future🤞🏻
@@JESUSSavedMefromDeath Aww thanks, that's really sweet ☺️ I'm not doing anything amazing but hopefully I can be a small bit useful to someone. I'm sure you are doing great too.
Any one types, its not important but, remember that the suspension cells must be centrifuged at 200 rotation per min (rpm) for 5 min. With my ❤ for you.
Controls are essential in cell culture experiments to ensure the validity and reliability of your results. Here are the key times when controls should be incorporated: • Before Experiment Initiation - Cell Line Authentication - Mycoplasma Testing - Media and Reagent Quality Control • During Experiment Setup - Positive and Negative Controls - Treatment Controls • Throughout the Experiment - Monitoring Cell Growth and Viability - Contamination Checks
Thank you, this video is perfect! I had training on this yesterday but with so many steps, it's tricky to remember everything. I'm currently studying for a Masters degree in Infectious Diseases and I have to use this technique for my Thesis project. Thanks again :)
***** for me no more humiliating from my supervisor, due to repeated asking about the technique :)
Did you obtain your Master's?
@@JESUSSavedMefromDeath I did indeed ☺️ Studying towards the next qualification now. It feels like it never ends but hopefully it pays off in the future🤞🏻
@@TheBeesKnees1993 Congratulations! I am happy for you and it will definetely pay off! Good Luck in everything!!! You're an inspiration!
@@JESUSSavedMefromDeath Aww thanks, that's really sweet ☺️ I'm not doing anything amazing but hopefully I can be a small bit useful to someone.
I'm sure you are doing great too.
Hello. I have a practical work with MDA-MB-231 cells to perform, could you tell me for how long do you incubate them at 2:10 ? Thanks in advance
What is the composition of the liquid used for discharge cells that are not used anymore in the process ?
The music is so soothing lol
Nice cell culture
I am interested in finding out if the smallest surface ( that with volume signs) of the flask is treated for cell culture.
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By this method active viruses are produced or inactive viruses ???
Hematopoietic stem cell harvest apheresis is a special form of leukapheresis used to collect cells from the peripheral blood.
very helpful
great
Thanks for the video, it was really helpful.
excellent video
Perfect ❤️
What type of centrifuge was used in this video?
Any one types, its not important but, remember that the suspension cells must be centrifuged at 200 rotation per min (rpm) for 5 min.
With my ❤ for you.
Thanks a lot. This is great!
When are controls done with cell culture
Controls are essential in cell culture experiments to ensure the validity and reliability of your results. Here are the key times when controls should be incorporated:
• Before Experiment Initiation
- Cell Line Authentication
- Mycoplasma Testing
- Media and Reagent Quality Control
• During Experiment Setup
- Positive and Negative Controls
- Treatment Controls
• Throughout the Experiment
- Monitoring Cell Growth and Viability
- Contamination Checks
nice video, thanks
brriliant!!
Nice work...
perfect
Is it the same if the medium used is RPMI?
Yes
It is crucial to understand that the protocol differs based on the cells and not on the type of media we are using.
thank to this video, I understand cell culture beter
great job
i wonna ask a question
what is the function of EDTA??
in case someone wonder that too , dmso is basically antifrost
@@TheCervelas EDTA
Thank youuu! this video is just perfect! Tout est clair pour moi maintenant en culture cellulaire
Sorry the images are not clear and the music on a scientific video could be skipped and replaced by clear guidance.
Thanks for the video.
It taught me perfectly.
Big thump up to you!
App
Very informative this video. Thank you!
Perfect 👌🏻