Pour Plating

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  • เผยแพร่เมื่อ 25 ก.ย. 2016
  • A short instructional video to teach you how to sub culture bacteria by pour plating.

ความคิดเห็น • 33

  • @Bettersucksaul
    @Bettersucksaul ปีที่แล้ว +3

    Thanks I'm adding this to my skillset when applying to jobs because I did my micro lab so long ago and at this point no one wants to hire if I'm honest and say I'd need to be retaught 😐

  • @gayathrisasirakotaru1768
    @gayathrisasirakotaru1768 5 ปีที่แล้ว +1

    Thanks

  • @jm5462
    @jm5462 4 ปีที่แล้ว +1

    thank you :)

  • @learnandshare9292
    @learnandshare9292 3 ปีที่แล้ว +3

    The explaination was very good and clear.
    But doesn't glove is required while dealing with any kind of sample as per aseptic technique?

    • @microbiologyattheuniversit6847
      @microbiologyattheuniversit6847  3 ปีที่แล้ว +10

      This depends on the sample and what you are trying to achieve. Gloves may be worn but in some circumstances, gloves could be considered more dangerous (particularly if worn by someone who is inexperienced) close to a Bunsen burner flame. We suggest you refer to the specific guidance that will be provided in the laboratory you are working in and may depend on the organisms you are working with.

    • @Luis-bo2uj
      @Luis-bo2uj 2 ปีที่แล้ว +1

      gloves for pussies ;)

  • @sweetyissorandom1305
    @sweetyissorandom1305 6 ปีที่แล้ว +3

    did we mixed 1ml bacteria with the agar??? or mixed agar with bacteria??

  • @kimisaac5461
    @kimisaac5461 2 ปีที่แล้ว +2

    How come pour plate method is more time-consuming than spread or streak methods?

    • @microbiologyattheuniversit6847
      @microbiologyattheuniversit6847  11 หลายเดือนก่อน

      I think they probably all take around the same time. I guess it depends on how you work if this one is taking longer for you. Do you mean because you have to wait for the agar to set? In the other methods the agar has already been prepared and set so we don't need to worry about that time here.

  • @aasthashah4315
    @aasthashah4315 3 ปีที่แล้ว

    Why the bottle cap part is heated? I didn't get that one.

    • @microbiologyattheuniversit6847
      @microbiologyattheuniversit6847  3 ปีที่แล้ว +3

      When we use glassware we flame the neck of the bottles to remove any microorganisms that could potentially cause contamination. This is part of our aseptic technique. We would only do this with glassware though, not if we are using single use plastics.

  • @nurraihanaqilah8117
    @nurraihanaqilah8117 ปีที่แล้ว

    how many ml of molten agar you put inside the bottle before mixing with the bacteria?

    • @microbiologyattheuniversit6847
      @microbiologyattheuniversit6847  11 หลายเดือนก่อน

      This can depend on the sample and what we are trying to achieve but typically this would be around 20 mL.

  • @juliusianaquinobaya2892
    @juliusianaquinobaya2892 2 ปีที่แล้ว

    "While method allows for growth of anaerobic as well as aerobic bacteria. Any obligate aerobes which end up within the agar will not grow". How and why is this?

    • @microbiologyattheuniversit6847
      @microbiologyattheuniversit6847  2 ปีที่แล้ว +4

      There is likely to be less oxygen within the agar than there is in the air that the surface of the agar is exposed to. So it is probably better to say that obligate aerobes are more likely to grow on the surface of the agar than within the agar and that aerobes that need higher concentrations of oxygen to successfully grow would not be present within the agar if there is insufficient oxygen there for them. For anaerobes to grow, an anaerobic cabinet or maybe anaerobic jars would be required.

  • @CatDawson1
    @CatDawson1 3 ปีที่แล้ว +9

    Great video but yet another where gloves are forgotten about. They a basic requirement so why not add them in!

    • @microbiologyattheuniversit6847
      @microbiologyattheuniversit6847  3 ปีที่แล้ว +3

      We consider the sample we are working with, what we are trying to achieve and who is carrying out the work when deciding whether to wear gloves. Gloves may be worn and there are times they are essential but in some circumstances, gloves could be considered more dangerous (particularly if worn by someone who is inexperienced) close to a Bunsen burner flame.

    • @averyiker9137
      @averyiker9137 2 ปีที่แล้ว

      i realize I am kind of randomly asking but does anyone know a good site to watch newly released movies online?

    • @alejandrowesson3676
      @alejandrowesson3676 2 ปีที่แล้ว

      @Avery Iker Meh try Flixportal. just search on google after it:P -alejandro

    • @averyiker9137
      @averyiker9137 2 ปีที่แล้ว

      @Alejandro Wesson thanks, signed up and it seems to work :D I really appreciate it!!

    • @alejandrowesson3676
      @alejandrowesson3676 2 ปีที่แล้ว

      @Avery Iker glad I could help :D

  • @VLogKeluargaArsyla
    @VLogKeluargaArsyla 3 ปีที่แล้ว +2

    alhamdulillah hadir

  • @Gts2pro
    @Gts2pro หลายเดือนก่อน

    Whilst that’s great ! But aseptic conditions is about minimal contamination… so why have you no gloves on ?

    • @microbiologyattheuniversit6847
      @microbiologyattheuniversit6847  หลายเดือนก่อน

      Agree, when working aseptically gloves can be useful to minimise contamination. However, when teaching these techniques to inexperienced students the wider balance of risks are considered which includes the hazard of the Bunsen burner flame. At this level, working with low risk organisms, we make the judgement that it is better not to wear gloves due to the risk of burns due to the potential of the flame to melt the glove onto skin. These decisions take all this into account and if we were working with more harmful organisms we might insist on gloves.

  • @mrpeanutbutter6094
    @mrpeanutbutter6094 3 ปีที่แล้ว +1

    No gloves. It’s okay. Sometimes gloves can catch on fire

  • @varshac8212
    @varshac8212 3 ปีที่แล้ว

    I am microbiology grp

  • @gufranraaj7978
    @gufranraaj7978 5 ปีที่แล้ว +1

    Talrnted

  • @ayat4522
    @ayat4522 3 ปีที่แล้ว

    no gloves 🙂

    • @microbiologyattheuniversit6847
      @microbiologyattheuniversit6847  3 ปีที่แล้ว +1

      We consider the sample we are working with, what we are trying to achieve and who is carrying out the work when deciding whether to wear gloves. Gloves may be worn and there are times they are essential but in some circumstances, gloves could be considered more dangerous (particularly if worn by someone who is inexperienced) close to a Bunsen burner flame.

  • @bintangnovelio1412
    @bintangnovelio1412 11 หลายเดือนก่อน

    wear ur gloves!!

    • @microbiologyattheuniversit6847
      @microbiologyattheuniversit6847  11 หลายเดือนก่อน

      We consider the sample we are working with, what we are trying to achieve and who is carrying out the work when deciding whether to wear gloves. Gloves may be worn and there are times they are essential but in some circumstances, gloves could be considered more dangerous (particularly if worn by someone who is inexperienced) close to a Bunsen burner flame.