Thanks I'm adding this to my skillset when applying to jobs because I did my micro lab so long ago and at this point no one wants to hire if I'm honest and say I'd need to be retaught 😐
This depends on the sample and what you are trying to achieve. Gloves may be worn but in some circumstances, gloves could be considered more dangerous (particularly if worn by someone who is inexperienced) close to a Bunsen burner flame. We suggest you refer to the specific guidance that will be provided in the laboratory you are working in and may depend on the organisms you are working with.
I think they probably all take around the same time. I guess it depends on how you work if this one is taking longer for you. Do you mean because you have to wait for the agar to set? In the other methods the agar has already been prepared and set so we don't need to worry about that time here.
When we use glassware we flame the neck of the bottles to remove any microorganisms that could potentially cause contamination. This is part of our aseptic technique. We would only do this with glassware though, not if we are using single use plastics.
"While method allows for growth of anaerobic as well as aerobic bacteria. Any obligate aerobes which end up within the agar will not grow". How and why is this?
There is likely to be less oxygen within the agar than there is in the air that the surface of the agar is exposed to. So it is probably better to say that obligate aerobes are more likely to grow on the surface of the agar than within the agar and that aerobes that need higher concentrations of oxygen to successfully grow would not be present within the agar if there is insufficient oxygen there for them. For anaerobes to grow, an anaerobic cabinet or maybe anaerobic jars would be required.
We consider the sample we are working with, what we are trying to achieve and who is carrying out the work when deciding whether to wear gloves. Gloves may be worn and there are times they are essential but in some circumstances, gloves could be considered more dangerous (particularly if worn by someone who is inexperienced) close to a Bunsen burner flame.
Agree, when working aseptically gloves can be useful to minimise contamination. However, when teaching these techniques to inexperienced students the wider balance of risks are considered which includes the hazard of the Bunsen burner flame. At this level, working with low risk organisms, we make the judgement that it is better not to wear gloves due to the risk of burns due to the potential of the flame to melt the glove onto skin. These decisions take all this into account and if we were working with more harmful organisms we might insist on gloves.
We consider the sample we are working with, what we are trying to achieve and who is carrying out the work when deciding whether to wear gloves. Gloves may be worn and there are times they are essential but in some circumstances, gloves could be considered more dangerous (particularly if worn by someone who is inexperienced) close to a Bunsen burner flame.
We consider the sample we are working with, what we are trying to achieve and who is carrying out the work when deciding whether to wear gloves. Gloves may be worn and there are times they are essential but in some circumstances, gloves could be considered more dangerous (particularly if worn by someone who is inexperienced) close to a Bunsen burner flame.
Thanks I'm adding this to my skillset when applying to jobs because I did my micro lab so long ago and at this point no one wants to hire if I'm honest and say I'd need to be retaught 😐
thank you :)
Thanks
The explaination was very good and clear.
But doesn't glove is required while dealing with any kind of sample as per aseptic technique?
This depends on the sample and what you are trying to achieve. Gloves may be worn but in some circumstances, gloves could be considered more dangerous (particularly if worn by someone who is inexperienced) close to a Bunsen burner flame. We suggest you refer to the specific guidance that will be provided in the laboratory you are working in and may depend on the organisms you are working with.
gloves for pussies ;)
did we mixed 1ml bacteria with the agar??? or mixed agar with bacteria??
mixed 1ml bacteria with agar
You could do this either way. The important thing is to ensure they are well-mixed.
How come pour plate method is more time-consuming than spread or streak methods?
I think they probably all take around the same time. I guess it depends on how you work if this one is taking longer for you. Do you mean because you have to wait for the agar to set? In the other methods the agar has already been prepared and set so we don't need to worry about that time here.
how many ml of molten agar you put inside the bottle before mixing with the bacteria?
This can depend on the sample and what we are trying to achieve but typically this would be around 20 mL.
Why the bottle cap part is heated? I didn't get that one.
When we use glassware we flame the neck of the bottles to remove any microorganisms that could potentially cause contamination. This is part of our aseptic technique. We would only do this with glassware though, not if we are using single use plastics.
alhamdulillah hadir
"While method allows for growth of anaerobic as well as aerobic bacteria. Any obligate aerobes which end up within the agar will not grow". How and why is this?
There is likely to be less oxygen within the agar than there is in the air that the surface of the agar is exposed to. So it is probably better to say that obligate aerobes are more likely to grow on the surface of the agar than within the agar and that aerobes that need higher concentrations of oxygen to successfully grow would not be present within the agar if there is insufficient oxygen there for them. For anaerobes to grow, an anaerobic cabinet or maybe anaerobic jars would be required.
Great video but yet another where gloves are forgotten about. They a basic requirement so why not add them in!
We consider the sample we are working with, what we are trying to achieve and who is carrying out the work when deciding whether to wear gloves. Gloves may be worn and there are times they are essential but in some circumstances, gloves could be considered more dangerous (particularly if worn by someone who is inexperienced) close to a Bunsen burner flame.
i realize I am kind of randomly asking but does anyone know a good site to watch newly released movies online?
@Avery Iker Meh try Flixportal. just search on google after it:P -alejandro
@Alejandro Wesson thanks, signed up and it seems to work :D I really appreciate it!!
@Avery Iker glad I could help :D
Whilst that’s great ! But aseptic conditions is about minimal contamination… so why have you no gloves on ?
Agree, when working aseptically gloves can be useful to minimise contamination. However, when teaching these techniques to inexperienced students the wider balance of risks are considered which includes the hazard of the Bunsen burner flame. At this level, working with low risk organisms, we make the judgement that it is better not to wear gloves due to the risk of burns due to the potential of the flame to melt the glove onto skin. These decisions take all this into account and if we were working with more harmful organisms we might insist on gloves.
Talrnted
no gloves 🙂
We consider the sample we are working with, what we are trying to achieve and who is carrying out the work when deciding whether to wear gloves. Gloves may be worn and there are times they are essential but in some circumstances, gloves could be considered more dangerous (particularly if worn by someone who is inexperienced) close to a Bunsen burner flame.
I am microbiology grp
No gloves. It’s okay. Sometimes gloves can catch on fire
wear ur gloves!!
We consider the sample we are working with, what we are trying to achieve and who is carrying out the work when deciding whether to wear gloves. Gloves may be worn and there are times they are essential but in some circumstances, gloves could be considered more dangerous (particularly if worn by someone who is inexperienced) close to a Bunsen burner flame.