this video describes the process of aminoacylation of tRNA .It also describes the structural aspect of the tRNA synthetase and how does the reaction takes place in the active site
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The Shine-Dalgarno-sequence starts translation only in prokaryotes. In eukaryotes, the Kozak sequence takes over the function of the Shine-Dalgarno sequence. The video is easily misunderstood; in a way that you could think translation is the same in prokaryotes and eukaryotes (e.g. 0:25) - all the elongation factors etc. are very different. This sould be clarified
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Sir I have a doubt which structure of tRNA is used in Translation Tertiary L shaped or clover leaf model?because is secondary structure is functional one?
Very good question. please refer to this article to know more www.ncbi.nlm.nih.gov/pmc/articles/PMC2270698/#:~:text=Aminoacyl%2DtRNA%20synthetases%20(aaRSs),(aa%2DAMP)%20intermediate.
Theoretically If there are no available amino acids to charge tRNA (transfer RNA), protein synthesis cannot proceed effectively. But inside a cell there is no such biological condition that amino acids would not be there completely.
Charging a tRNA molecule with an amino acid requires the hydrolysis of one molecule of ATP to AMP and pyrophosphate. So, one ATP molecule is typically used to charge a tRNA molecule with an amino acid.
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why is every specific video about science made by an Indian dude ? its mind blowing
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Lol
The Shine-Dalgarno-sequence starts translation only in prokaryotes. In eukaryotes, the Kozak sequence takes over the function of the Shine-Dalgarno sequence. The video is easily misunderstood; in a way that you could think translation is the same in prokaryotes and eukaryotes (e.g. 0:25) - all the elongation factors etc. are very different. This sould be clarified
short and concise, have saved me the stress
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Longing for this video tq bro
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Thanks so much for this video¡
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Really good 👍
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THX!
Sir I have a doubt which structure of tRNA is used in Translation Tertiary L shaped or clover leaf model?because is secondary structure is functional one?
Happy Saraswati puja sir
Thanks... same to you
I just have a doubt sir, if he tRNA is charges with amino acid why does translation occurs since it now already has the amino acid?
iRNK just gives information what Aminoacids need to be connected in order to make polypeptide aka Protein. Which in case, tRNA brings to the ribosome
you call it translation in first few seconds instead of transcription. Is this an overview or purposeful?
Sir how does the enzyme aminoacyl tRNA synthetase know which amino acid to charge?
Very good question. please refer to this article to know more www.ncbi.nlm.nih.gov/pmc/articles/PMC2270698/#:~:text=Aminoacyl%2DtRNA%20synthetases%20(aaRSs),(aa%2DAMP)%20intermediate.
Nice vdeo sir
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Where in the cell does aminoacylation occur? In the nucleus or in the cytosol?
Sarah Dickson cytosol and it makes sense because in eukaryotes the translation is happening in the cytoplasm as well
In the cytosol
How CAA residue added to trna post transcriptionally?
It's done by CCA adding enzymes called nucleotidyl transferases
What is discharging of trna and how does it take place
Interesting question we would address this in a video
Does the sedimentation of trna and aminoacylation refer to the same thing?
No
Then can you please explain what is sedimentation of trna? I didn't get any accurate answer by far... thank you!🥺
How does t rna recognise which amino acid is to be taken
Very nice question...I would answer it in a video...this is also known as second genetic code
The mRNA coding sequence directs trna to bring in a specific amino acid based on the fact that anticodons are complementary to the codons.
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What if there is no amino acid available to charge trna
Theoretically If there are no available amino acids to charge tRNA (transfer RNA), protein synthesis cannot proceed effectively. But inside a cell there is no such biological condition that amino acids would not be there completely.
Is only one ATP utilized in tRNA charging, or are two ATP utilized in tRNA charging
Charging a tRNA molecule with an amino acid requires the hydrolysis of one molecule of ATP to AMP and pyrophosphate. So, one ATP molecule is typically used to charge a tRNA molecule with an amino acid.
ok thank you sir@@animatedbiologywitharpan
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You are having a hard time speaking in English. But informative video though. Thank you.
Not a native English speaker.....sorry about it...I hope the content was useful.
@@animatedbiologywitharpan Your English is quite good don't worry! :)
@@CodySchmitt that’s reassuring....I am trying to improve every day...please share my channel link with your friends and help me to reach big audience