How u prepare the sample for analysis.. I have seen preperation of mobile phase, vitamin c standard preperation and running procedure of machines.. but not seen how sample is selected
Hi, sorry but I have the question regarding the formula. I understand the calculation but somehow I'm confused on the dilution factor part, the dilution factor is mark up volume, mL divided with mass, g: hence in final calculation shouldn't the unit is still there? Meaning that we still have the ppm × ml/g unit? How can we just end up with ppm value since we haven't cancel out the ml/g unit. Please help me on this. Thanks a lot
Hello maam If I send my sample for quantification and method development to a institutions,should I provide methodology? They gave me the results but the peaks are not good.. They also charged 15k for method development because the methods i provided did not work.. If they have developed the method the peak should come good m I right sir Actually my sample was a formulation that I developed
You shouldn't provide methodology to the analytical laboratory while you are paying for the test. Method development includes best quality of chromatogram with a sharp and nice peak.
@@MicroChemsExperiments in case of broad peak can i calculate the concentration or not I have been in contact with the scientist of that institution. He tells that it's ok. They have run 3 times so no problem. I can put it in paper also
Maam if chromatogram show more peaks than the desired compund ..what does it mean . Can It gives the wrong result for the quantification of our desired compund. Please rply And is it necessary to get relative area percentage 100% for the quantification of the desired compound.
Your chromatogram may contain many peaks with different retention time. You should consider only that peak of retention time which match with the retention time of standard peak
hello, how are you
what is the standard method please?
Hi ma'am I have one doubt Your using column is c18 it is phenomenex column or any other brands
Superb... Spike and recovery video also do that for vitamins and cholesterol
Ok we will do it. Stay with us.
Dear, could you share the methodology on which you based it please?
How u prepare the sample for analysis.. I have seen preperation of mobile phase, vitamin c standard preperation and running procedure of machines.. but not seen how sample is selected
Sample preparation: th-cam.com/video/aB5B3lp-GoY/w-d-xo.html
Hi, sorry but I have the question regarding the formula. I understand the calculation but somehow I'm confused on the dilution factor part, the dilution factor is mark up volume, mL divided with mass, g: hence in final calculation shouldn't the unit is still there? Meaning that we still have the ppm × ml/g unit? How can we just end up with ppm value since we haven't cancel out the ml/g unit. Please help me on this. Thanks a lot
We found a unit from the dilution factor, which is ml/g. And the ppm means g/ml. So both units will cut each other.
Maam is the ç 2 is the concentration of vit c in 20 microL injected sample or amount of vitamin c in injected 20 microL sample
C2 is concentration of vitamin c in your sample
@@MicroChemsExperiments thank you
wow thanks
You are welcome.
Good sir
Keep watching
Hello maam
If I send my sample for quantification and method development to a institutions,should I provide methodology?
They gave me the results but the peaks are not good..
They also charged 15k for method development because the methods i provided did not work..
If they have developed the method the peak should come good m I right sir
Actually my sample was a formulation that I developed
You shouldn't provide methodology to the analytical laboratory while you are paying for the test.
Method development includes best quality of chromatogram with a sharp and nice peak.
@@MicroChemsExperiments that means they are unable to develope the methods...
Peaks are not sharp and this is my last semester..what should I d maam
@@surjitdeka7092 Contact with them (Testing Service Provider)
@@MicroChemsExperiments in case of broad peak can i calculate the concentration or not
I have been in contact with the scientist of that institution. He tells that it's ok. They have run 3 times so no problem. I can put it in paper also
Hi! According to which authors is this mehod?
We combined several journal and modified in our lab to develop this suitable method.
Maam if chromatogram show more peaks than the desired compund ..what does it mean . Can It gives the wrong result for the quantification of our desired compund. Please rply
And is it necessary to get relative area percentage 100% for the quantification of the desired compound.
Your chromatogram may contain many peaks with different retention time. You should consider only that peak of retention time which match with the retention time of standard peak
@@MicroChemsExperiments thank you
@@MicroChemsExperiments that mean we should only consider the peak that is match with our desired compund
Hi. Is there anyway to determine a assay of vitamins. Like titration/titrimetry
Yes. Tritrametric method is also available. We will upload later
@@MicroChemsExperiments Cool! this could really help! thank you so much! Keep it up and God Bless. 🤩
@@MicroChemsExperiments and other vitamins as well.
Can i determine the percentage of vitamin c in tomato paste please tell me ?
Yes. You can
Ma'am please clear my doubt
reference de methode svp😢
No specific reference. It is a Laboratory developed in-house method
Sir please make a video for gravimetric titration
Noted.
Hello. Great job. Do you run fb page? İ' ve send you msg but there are not answer..
Sorry for late. I will reply soon.