In ESI, with a single pure proteofrom being ionised, each peak will represent a different charge state of the protein, with a smaller m/z being a higher charge state (more H+ attached). To work out the mass of the protein, simultaneous equations can be used and this can be done manually with enough time. Software that comes with the MS often does this, but there is also online calculators like this one: www.bioprocess.org/esiprot/esiprot_form.php.
Hello Dr. I will like to know if you have a published paper concerning this overview because I will like to use it as one of my references for a research paper I'm writing on "Translational Proteomics with Advanced Mass Spectrometry (MS) Systems".
This is all out of my own head using articles and text books that were already out there, along with discussions with engineers. So I don't have a single article. If you email me, I'll try and find some srticles to send you.
Bwahahahahahahaha! No, I'm not a smoker. But the audio was recorded at about 7am in my office by myself with a headset. I realise that I need to be more dynamic.
Four years and someone finally picks up a 'mistake'. You tell me what the common name for an uncharged ion is, one that is able to enter the MS, be past through the instrument and able to strike the detector, giving a false signal? An uncharged molecule, I guess.
This is a great summary but there are some serious copyright infringement issues with this video. The simulations in the LC-MS are straight from Thermo Scientific. There is no credit attributed to Thermo anywhere in the video and it is not clear that permission has been given for use of their video.
Somebody please get this guy a glass water.
THANK YOU
I can't even focus on what he's saying because all I hear are those wet mouth noises that melt my brain
Jesus Christ 🙏😂
😂😂😂😂😂
Makes it unwatchable, unfortunately
Took me a while to find this explanation. Perfect overview of the subject.
This is absolutely AMAZING. Thank you and please make more videos science needs you.
A comprehensive overview of Mass spectrometry.
Good job Matthew.
Very great explanation! Thanks- now I get an idea of how mess spectrometry works for biomolecules.
Very well done! great amount of info for under 30 min.
Thank you! You are so good at explaining honestly.
Great video! I learned a lot, thank you for the hard work.
Thanks Matthew, this great material!!
Every protein man deserves such an ultimate perfect instruction.
Best explanation I saw. Congrats!
fukken brilliant m8. thanks a lot for the detailed intro.
Thank you so much, I definitely owe you one 👏🙌
Thank you so much! I needed this for my uni course.
Bloody legend mate
Thank you!!!!
thanks so much
thank you for uploading
thank you so much!!!
How can one determine the molecular mass of protein by the peaks given in graph?
In ESI, with a single pure proteofrom being ionised, each peak will represent a different charge state of the protein, with a smaller m/z being a higher charge state (more H+ attached). To work out the mass of the protein, simultaneous equations can be used and this can be done manually with enough time. Software that comes with the MS often does this, but there is also online calculators like this one: www.bioprocess.org/esiprot/esiprot_form.php.
Cheers!
elaborate explanation
This is a general theoretical on mass spectrometry. Would be more useful if top-down and bottom-up approaches in proteomics were covered.
Yes. It is on the to-do list. Hopefully in the next few weeks, these will start to be updated. They take a long time to get done properly.
Hello Dr.
I will like to know if you have a published paper concerning this overview because I will like to use it as one of my references for a research paper I'm writing on "Translational Proteomics with Advanced Mass Spectrometry (MS) Systems".
This is all out of my own head using articles and text books that were already out there, along with discussions with engineers. So I don't have a single article. If you email me, I'll try and find some srticles to send you.
Brilliant but good lord it sounds like you're presenting to the Top 30 CEOs of the Fortune 500. Get some water homie.
You don't like the sexy huskiness? ;)
Great information, thank you, but kindly are you a smoker Dr?
Bwahahahahahahaha! No, I'm not a smoker. But the audio was recorded at about 7am in my office by myself with a headset. I realise that I need to be more dynamic.
@@MatthewPadula oh great, I just thought you had breathing difficulty, I would not even be speaking that early and again thank you for the videos. 😊
HOW DID YOU IDENTIFIED THESE ARE ISOTOPES OF CARBON
Great video, but this guy needs to take some pauses in between!
@16:46 What the heck is an "uncharged ion"????????
Four years and someone finally picks up a 'mistake'. You tell me what the common name for an uncharged ion is, one that is able to enter the MS, be past through the instrument and able to strike the detector, giving a false signal? An uncharged molecule, I guess.
The Thermo video (th-cam.com/video/KjUQYuy3msa/w-d-xo.html) is no longer available.
Remind me where this is referred to in the video.
@@MatthewPadula 20:17
@@SethCrosby Thanks. I will fix it for the next version.
like and subscribed 👍
go from the simple thing, don't mix the stafs
Myoglobin 11:35
dada
deeeeeeeyta you say, huh?
This is a great summary but there are some serious copyright infringement issues with this video. The simulations in the LC-MS are straight from Thermo Scientific. There is no credit attributed to Thermo anywhere in the video and it is not clear that permission has been given for use of their video.
Pffft