This is absolutely fantastic, thankyou so much for putting this together so nicely edited. Even though we use different instrumentation, different samples and analytes and software, the science remains the same. Thanks heaps!
Mam there is a caption under your PHENOMENEX logo that is "BREAKING WITH TRADITION" Is there any scientific meaning in that I want to know the meaning of the caption my thought about this caption is "IN LIQUID CHROMATOGRAPHY SEPARATION OF COMPOUNDS BASED ON THE RELATION OF MOLECULES WITH COLUMN MOLECULES ie. RELATIVE AFFINITY" So the compounds present in the sample will break relation with column materials slowly and elute one by one.
in looking for natural products compounds for specific properties; - even if literature provides possible masses of compounds, there is not enough material to do a tune. What is the best approach for this situation?. Fractions of plant extractions were collected, and would like to analyze these fractions at possible masses found in the literature to start. So do not really know the mass, and volume is approx 50ul or so. Thank you
Can we optimize the parameters for drug and Internal standard at the same time, using a mix of drug and ISTD at around 100ng/ml..? I really got surprised to see the DP optimization after the CE optimization. Will definitely try this way next time in my lab. Thanks a lot, ma'am. Regards from India 🇮🇳
Hello! Unfortunately, the scientist who starred in that video no longer works in our lab. However, SCIEX does recommend 5-10ul/min. You can find their full manual here sciex.com/x51122. I hope that helps! However, if it doesn't, please reach out to our technical experts at Live Chat at www.phenomenex.com/chat.
I wanted to learn to how to use it when i was working on my research. My lab attended left me with the machine without any instructions. I ended up jamming all tubing due to solid particles. In the end i had to pay 100$/sample to other lab, to get readings.
You must use MS grade solvents only and filter through 0.22um membranes ideally. This avoids particulates. Where is the machine located? People at your lab who know how to run these instruments forget how challenging it can be to become proficient.
Hi. Could you made other video, explaining how to process using UHPLC HRMS/MS, for give attribution to unknown compounds the formule,retention times, fragment ions, becuase when you run a sample and process the base peak (ms1) you have retention time, but in ms2 you have other rt of the some and not are the same from the chromatogram.I hope you can understand want I can to say. By the way great video:)
We are so happy that you found this video helpful! Thank you for that:) I reached out to our partners at SCIEX regarding your question. Here is their reply: "With high res instruments, most people don’t really do tuning in the same way as for QqQ instruments. There are a couple of reasons for this: 1. Usually HR instruments are used for more qualitative work, so the quantitative needs are not as great 2. Generally quant is done from the ToF scan, so fragmentation is not used for quant (although it can be!) If someone wants to do HRMS quant in a way similar to a QqQ, what we call MRM-HR, the tuning process is similar. They need to ramp collision energy to get efficient fragmentation. It is a little different because there will be one CE setting to generate and monitor multiple fragments, not a CE for each MRM. Other parts of the question are a bit confusing, I’m not sure how to interpret." Here’s a video link explaining the scan. sciex.com/community/application-discussions/technology/qtof/fundamentals/multiple-reaction-monitoring-high-resolution-mrm-hr-scan-function Hope this was helpful!
Are these things able to be manipulated/hacked to influence the results? I looked at a piece of research that I'm concerned about the origin of the machine....
This is absolutely fantastic, thankyou so much for putting this together so nicely edited. Even though we use different instrumentation, different samples and analytes and software, the science remains the same. Thanks heaps!
" 'Tune' in for the next video". Nice one
Well done. Nice workflow and everyday-practical vs exhausting!
Thank you for explaining basics.
very helpful! thanks!
Thanks I just infused Dopamine into my Mass Spec with this video!!!
Thanks for this great video!
For every changes you did in the computer sample was being drawn right from the tube? Like it was not used instantly?
great Video, could you also Explain the Compund Optimization and FIA?
BR
Mam there is a caption under your PHENOMENEX logo that is "BREAKING WITH TRADITION"
Is there any scientific meaning in that
I want to know the meaning of the caption
my thought about this caption is "IN LIQUID CHROMATOGRAPHY SEPARATION OF COMPOUNDS BASED ON THE RELATION OF MOLECULES WITH COLUMN MOLECULES ie. RELATIVE AFFINITY"
So the compounds present in the sample will break relation with column materials slowly and elute one by one.
Nice explanation. could you please show us method validation for LC-MS I want to estimate the concentration of drug in the sample
Does anyone know the brand of that column washing machine?
in looking for natural products compounds for specific properties; - even if literature provides possible masses of compounds, there is not enough material to do a tune. What is the best approach for this situation?. Fractions of plant extractions were collected, and would like to analyze these fractions at possible masses found in the literature to start. So do not really know the mass, and volume is approx 50ul or so. Thank you
Thank you so much ma'am 🙏
When tuning analytes concentration for the API 6500?
Thank you very much for this usefull video.
Declustring potential must be same for all transition.?
What is the benifit of multiple channel averaging?
DP will vary from compound to compound. MCA just ensures a good average is obtained and reduces variation in apex of peak
Cool! Thank you! Very professional! Best regards!
Hi there!
So for unknown compounds, like for natural products. How do run an MS/MS? Thanks...
Can we optimize the parameters for drug and Internal standard at the same time, using a mix of drug and ISTD at around 100ng/ml..? I really got surprised to see the DP optimization after the CE optimization. Will definitely try this way next time in my lab. Thanks a lot, ma'am.
Regards from India 🇮🇳
Please explain Source gas parameters also
What rate are you pumping at (I have a manual syringe pump)? 100 ng/ml @ 5 microliters/min? 20? 200?
Hello! Unfortunately, the scientist who starred in that video no longer works in our lab. However, SCIEX does recommend 5-10ul/min. You can find their full manual here sciex.com/x51122. I hope that helps! However, if it doesn't, please reach out to our technical experts at Live Chat at www.phenomenex.com/chat.
Mam How to identify that Molecules are consist Positive polarity or Negative Polarity??
Would you like to sell this used system? I am interesting to buy this used equipment.
Very interesting 👌👌
Best video
I wanted to learn to how to use it when i was working on my research. My lab attended left me with the machine without any instructions. I ended up jamming all tubing due to solid particles. In the end i had to pay 100$/sample to other lab, to get readings.
You must use MS grade solvents only and filter through 0.22um membranes ideally. This avoids particulates. Where is the machine located? People at your lab who know how to run these instruments forget how challenging it can be to become proficient.
Hi. Could you made other video, explaining how to process using UHPLC HRMS/MS, for give attribution to unknown compounds the formule,retention times, fragment ions, becuase when you run a sample and process the base peak (ms1) you have retention time, but in ms2 you have other rt of the some and not are the same from the chromatogram.I hope you can understand want I can to say. By the way great video:)
We are so happy that you found this video helpful! Thank you for that:) I reached out to our partners at SCIEX regarding your question. Here is their reply:
"With high res instruments, most people don’t really do tuning in the same way as for QqQ instruments. There are a couple of reasons for this:
1. Usually HR instruments are used for more qualitative work, so the quantitative needs are not as great
2. Generally quant is done from the ToF scan, so fragmentation is not used for quant (although it can be!)
If someone wants to do HRMS quant in a way similar to a QqQ, what we call MRM-HR, the tuning process is similar. They need to ramp collision energy to get efficient fragmentation. It is a little different because there will be one CE setting to generate and monitor multiple fragments, not a CE for each MRM. Other parts of the question are a bit confusing, I’m not sure how to interpret."
Here’s a video link explaining the scan.
sciex.com/community/application-discussions/technology/qtof/fundamentals/multiple-reaction-monitoring-high-resolution-mrm-hr-scan-function
Hope this was helpful!
thank you
Are these things able to be manipulated/hacked to influence the results? I looked at a piece of research that I'm concerned about the origin of the machine....
We offer turbo controller repair for Mass spec systems
Fentanyl! yummy! Nice demonstration.
More like...AB Seinfeld
speaks soooo fast