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😉

Thanks

To all women in Dundee, be VERY careful about this individual -----> warningtoallwomenindundeescotland.blogspot.com/

It's a shame that this video doesn't have as many views like other tips videos. A lot of these tips are really useful. I have been using many of these tips for some time now, but had I known about them from the beginning then my treatment would have been easier from the start.

I thought I was gonna see the process….

As my application of admission is accepted by the university of Dundee. The university authority is very cooperative but i need some serious or major help or assistance to travel and grab this opportunity.

Really helpful, thank you 🩷

Great video with so many facts and information from someone who grew up in the wonderful town of Stonehaven and has the surname Ramsay as my family previously ran the shop you referenced in the square. Now live in the USA so your production ideal to share with anyone I am trying to explain the unique qualities of Stoney

Wow thank youbfor this video that was super helpful 😁😊

You have explained this better in 10 minutes than an entire module on Western Blotting at university has!! Thank you!

3:32 I formed a loop and then I poo 💩 but my stitches came apart

What abt the teaching quality in masters, classroom strength, assessment, research quality and accommodation cost?

I have 25 trays and change weekly, hopefully stays that way

You’re so cute im dying

What does v mean? is it a control?

Hello, thank you very much for your video. Is it possible to make another one guiding students regarding renting as a student. I’m planning to start my studies this September but rent scams are scarring me as I am an international student.

Thank you, i accepted in dundee and i come in September, could you please tell me about renting house and fouding job

Thank you for this! 🙏🤩 helped me sew up the bottom of my jeans 🥰

"promo sm"

Where did you go?

I enjoyed the ironing board " hack!" Will move my board next to the window! Thanks! Cheers!

What is the importance, if any, of having one piece of thread for the length of an entire seam - as in a pant leg? I find it difficult to “wrangle” a long length of thread. It always gets tangled or makes an unwanted knot. Is it okay to sew an entire seam length with smaller pieces of thread or does it have to be one?

Pretty lucid presentation

One of the best biology explanations on TH-cam, thanks! Incredibly helpful

Great job!

Great video and nice explanation. Thank you!

Thank you for this, much needed tutorial for me to finish the hemming of curtains. Also a very happy burns week and night for the 25th Jan. Hopefully it's a real special one. All the best from one Scot to another 🏴󠁧󠁢󠁳󠁣󠁴󠁿

Amazing, finally a useful video for normal people without offering any products. Thank you!

thank you ❤ kisses from Brazil 😘

Thanks a lot for your video!

this is the best video on western blot. thanks...

If you don’t get sarcasm, you shouldn’t come to Dundee Uni 😊

Thank you so much! This tutorial was short, sweet, and to the point. I literally know nothing about sewing, but using this tutorial was able to repair my son's hoodie pocket. 🥳

Ahhh, finally a tutorial that shows how to hem curtains in a non-permanent way, no cutting, sewing machine or hem tape! I live in an apartment with baseboards and I will absolutely want my curtains back to their proper full length for one day when I have my own home, so I didn't want anything permanent that would require me to re-purchase down the line. Your tutorial showed me every step & question I might've needed answered and you've helped me so much, thank you!

im in year 10 right now, and after talking to some representatives of UoD and researching it myself, ive decided its my dream uni, i want to go into maxfax, so their dentistry course is very appealing to me.

Did you have to pay extra for refinements???

every don't is do for me

Thank you. This has been really helpful 😊

Thank you for clearly explaining the procedure, it really helped me a lot in understanding it.

Thank you so much! 🙂

Very great explanation. Very bad blot itself though 😅 A few recommendations. Some, you may already be doing: 1. BSA Protein Concentration Assay for all samples simultaneously prior to running them on a western. 2. Never run more than 15 ug of Protein per lane. If this is below your threshold of detection, switch to a commercial blocking buffer such as SuperBlock from Invitrogen in TBS-T. That should help with sensitivity. The reason that 15 ug should be the upper limit is because you have to be within the linear range of protein concentration for an antibody to be used for relative quantification. For a protein like GAPDH, this linear limit in many cells lines is 5 ug. So in addition, I would also use a different HSKG like beta actin or vinculin. 3. Instead of a housekeeping gene, you can also go with a total protein stain. You used Ponceau, but depending on the company you buy it from, it's really hit or miss. And if you make your own Ponceau as I used to, that's even less consistent between lots. Some people just forgo housekeeping genes all together and only go with the total protein stain, but it's also possible to use 2-3 housekeeping genes on the same blot so that you can take the best 2 of 3. If you have a problem with 2 of them, then you're either modifying expression of 2 of them OR your sample concentration was measured accurately. 4. if you heat your samples, don't go over 10 mins at 95C. Can cause hydrolysis of your protein and that can also effect how it shows up on the blot and potentially account for why your GAPDH isn't even. Should help you get more consistent results and thus graduate faster. Good luck!

THANK YOU SO MUCH. My favorite pants just ripped at the seam and you explained how to do a simple stitch so well! Im glad I can try to sew it myself at home with your amazing directions <3

I start my Invisalign around January time. Thank you for the tricks and tips. After you’ve completed all your trays how long do you wear the retainer for? 22 hours a day is go long. I hope I don’t gag

May I know how you differenciate between cells and debris?

This video was helpful. Thank you for sharing. 👍🏾

Scummy in the 80s? No. I studied at the Uni in the 80s and judging by the various YT videos I’ve recently watched, it was a lot better. No phones, but you simply stepped outside and had a great time with interesting people. It was wonderful.

Are your teeth sensite after ipr, i wanna know. I wanna do this instead of teeth extraction.

Bane of my bloody existence, western blots and live cell imaging

Hi Emma, just a little confused are the green and red splotches on the graphs the fluorescent given off by the antibodies? I’m finding interpreting the graphs confusing. Great vid thanks

haha