You should need to edit the title or add in description section about the cell type whether it is attached or suspension cell line. Because if it was suspension cell line, all the cell would be discarded as well by removing the media at first place.
Do you have any idea how to isolate the URNIE STEM CELLS by simple centrifugation? Usually, the stem cells are mixed with sediments in the morning urine- the best time for obtaining the urine stem cells. I resuspended the sediments -which disappeared at the next centrifugation , but it did not help to find any single urine stem cell. Usually, there are just 5 to 10 urine stem cells in 100 ml of fresh urine. I used a protocol with 500 g for 5 minutes of urine centrifugation.
The video is educative and interesting. However, if you are not of maintaining the sterility or not too sure of your working environment please revert to using flame at every point of your work.
KS LEE you mean open the lid slightly of the T-75, after placing in the incubator for air flow? It may be the T-75 where the cap has a filter, so you do not have to loosen it. Cheers
Why are you wasting your time spraying the pipette packaging? You aren't going to get it close to being completely sterile, and that wrapper should never be over any of your open containers, anyway.
You should need to edit the title or add in description section about the cell type whether it is attached or suspension cell line. Because if it was suspension cell line, all the cell would be discarded as well by removing the media at first place.
i agree with you
totally agreed!
Do you have any idea how to isolate the URNIE STEM CELLS by simple centrifugation? Usually, the stem cells are mixed with sediments in the morning urine- the best time for obtaining the urine stem cells. I resuspended the sediments -which disappeared at the next centrifugation , but it did not help to find any single urine stem cell. Usually, there are just 5 to 10 urine stem cells in 100 ml of fresh urine. I used a protocol with 500 g for 5 minutes of urine centrifugation.
Thank you so much 😊
The video is educative and interesting. However, if you are not of maintaining the sterility or not too sure of your working environment please revert to using flame at every point of your work.
There's no need for PBS if it's you're just doing a regular media change.
The t75 must remain with the cap on at all times except when pipetting
KS LEE you mean open the lid slightly of the T-75, after placing in the incubator for air flow? It may be the T-75 where the cap has a filter, so you do not have to loosen it.
Cheers
Shouldn't he spray his hands again with alcohol whenever he discards used pipettes under the cabinet?
No, that quick movement is fine
why are you not wearing a face mask?
How long do you change the medium?
Please mention what type of cells are ? Are human cell ?
If she discarded the media won't the cells go with the media?
the cells are adhered to the flask
Only the dead cells go with media, the adherent cells are fine. Which is good for the healthy of last one. I think.
good video.
great video indeed :)
am just start doing my phd work .... and this video will help me :)
sterile work = great data/result :) cheers :)
No need to wash cells with PBS?
In how many days we should change media
Mention 2-3 days.
you didn't wash the culture with PBS before adding the fresh media?
its very nice
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Why not open partially the T-75 in the CO2 incubator in the last part of the video?
KS Lee it's a vented flask
what kind of bracelet was he wearing? is it sterile? I think not... for shame, sir. for shame.. :( not :D
i'm looking for a cell culture lab for an internship..can any one help?
@Zauberberg
My bad I meant to dislike this. CSU Pomona does it hood status!
Why are you wasting your time spraying the pipette packaging? You aren't
going to get it close to being completely sterile, and that wrapper
should never be over any of your open containers, anyway.
just for precaution.
You shouldn't wear socks while performing experiments
it depends on how skilled you are and it depends on the cells.
CSU Pomona does it better!!!