Many thanks for this video..when I press images to stack it convert the green one into red..and then when I open the original image and make a montage..the resulted montage would be two red images (one originally green) and one black image (not the composite image)..can you please tell me how to fix that?
Don't know if still needed.. I had the same. when you change the type of the image to what is should be (32bit or idk what you had), it converts back to greyscale and then you can color-merge to get the desired colors for the signals.
Cammer Michael I agree ,for quatification the raw bioformat file is important but for a quick display one can save the rgb image .....well for intensity quantification saturation is a problem but for displaying a structural feature it might not be an issue. But I agree to the point that while imaging the full dynamic range of the detector should be utilised
Save my day! Lat time it took me hours to align and display images and now only 1 minute everything down! Really Thank you!
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Do you know how to calculate the collagen fragmentation index for confocal microscopy?
No my friend, I don't know this
Hi, can you please make a video on how to use Orientation J for fibre orientation measurement?
Many thanks for this video..when I press images to stack it convert the green one into red..and then when I open the original image and make a montage..the resulted montage would be two red images (one originally green) and one black image (not the composite image)..can you please tell me how to fix that?
Hi there. I seem to be having the same issue. Have you figured out how to rectify it?
Don't know if still needed.. I had the same. when you change the type of the image to what is should be (32bit or idk what you had), it converts back to greyscale and then you can color-merge to get the desired colors for the signals.
the desktop picture is great!
Thanks! Please share with your friends to help my channel grow...😇
NICE one Arpan!
Can u show how to deconvolve the confocal image stack with fiji and make it to 3D
You need the raw data with correct metadata and no saturation to do this.
How do you save pictures in RGB? Mine are in 'ND2' :c
Image 》 type》 rgb then go to files 》 save ad 》 tiff
Confocal images should be saved in different channels, not as RGB, and not be saturated.
See the recent Nature Methods paper by Jonkman et al.
Cammer Michael I agree ,for quatification the raw bioformat file is important but for a quick display one can save the rgb image .....well for intensity quantification saturation is a problem but for displaying a structural feature it might not be an issue. But I agree to the point that while imaging the full dynamic range of the detector should be utilised
Nice tutorial! I would like to know if you can put each image scale. Thanks
Yes you can I would soon put more videos on that
Good one
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Is there you can put a download link for the images?
hey, when i press stack all the pictures are green colour and the hyperstack just does not work :( PLS HELP
We can chat on WhatsApp if you want 9987997041
Arpan Parichha hey, can you just reply on here?
Katia Stas I don’t understand the problem you are facing...can you elaborate..
I understand CASA can be incorporated into ImageJ to analyze semen and sperm cells. Can you do a tutorial on that?
Thanks man!
Please don't save scientific image data as JPG files! Compression is not your friend
Ya recommended should be tiff
Thanks for pointing out
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