You've labelled your sample with multiple fluorophores and carefully taken pictures of each fluorophre. How do you put those images together into something you can publish?
Can i find any video about the calculation of Intensity and related things which are required to be kept in mind while quantifying intensity and comparing in different biological replicates? will love to see if there is any. i just found one such video here on youtube. which lacks most of things
Quick question: why do you have to convert the images to 8-bit, instead of working directly with the separated channels, which are already in red-green-blue?. I usually just merge the channels without need to covert the images to 8-bit before.
Hello sir,I want to use this software to measure the width of the cracks of the reinforced concrete beams, if possible to apply a grid (perpendicular to the crack) on the image this grid to determine the width and thank you in advance please idea me it's urgent.
Sir will you help me for some image processing, related to trichome (hair like structure on leaf) counting ? I am trying but not getting proper results.
Hi, I am working on phases of polymer blends using Atomic force microscopy. Please upload a video about how to filter the phase image so that we can easily differentiate phases of the blend..
I need to find fibers of a filament in the series of images. each image might have hundreds or thousands of these fibers and I have hundreds of images. What is the systematic approach that can find these fibers in the image?
Good to know about this video; you have presented very clearly. Would love to see videos for understanding co-localization analyses at Image J. Thank you !
Try this article. it is very useful but you need to download the plug - ins required for the segmentation www.jstage.jst.go.jp/article/plmorphol/29/1/29_15/_article/-char/ja Copy the title and search for the English version. It's available in Pdf format
Thanks. One more question: I need to find fibers of a filament in the series of images. each image might have hundreds or thousands of these fibers and I have hundreds of images. What is the systematic approach that can find these fibers in the image?
@@zeynabmousavi1736 it depends on what you are trying to investigate in the first place. For instance, in the above article the systematic approach is based on 4 parameters of the microfilaments. According to your test you must decide what the precise effect that you are suspecting to observe. Then you will be able to make analysis for the whole area of your images or for certain fields of your choice. Since you have plenty of them, then you must be comparing something against another. The values you get from the image analysis software can be translated into readable data using a statistical test of your preference.
@@andromeda3780 Lets explain in different way: I have fibers of the cytoskleton scattered inside an image. There are hundreds of those fibers in the image. Assuming that fibers are linear, I want the software to find all those fibers and find a specific characteristics of those fibers (for example find the angle between each fiber a vertical line in the image. So I would extract hundreds of angles from one image and I want to repeat this with several other images that I have. Also I might need to section an image and do the above analysis on a section of the image. Any recommendation in this case would be appreciated.
@@zeynabmousavi1736 in this case try this article journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.1005063 But any way the approach is the same whether you have one image, section of an image or hundreds of them. You must know how to run the analysis using software.
the voice and tone sounds like the listening test in TOEFL
Thanks for sharing.
Can you please make a video on how to get composite using z-stacks
why you have first change the cokor to gray scale?? n on final click it became automatically colored one?
Can i find any video about the calculation of Intensity and related things which are required to be kept in mind while quantifying intensity and comparing in different biological replicates? will love to see if there is any. i just found one such video here on youtube. which lacks most of things
Very good tute
Please do video of how to scale images using image j
Could you please tell me how to use pixel values at selected points in the image as numeric overlay?
Quick question: why do you have to convert the images to 8-bit, instead of working directly with the separated channels, which are already in red-green-blue?. I usually just merge the channels without need to covert the images to 8-bit before.
😮😢😮😮😮😮😮😢
Could you please upload how to process a large-sized video in imageJ
Can you please show how to quantify signal intensity inside and outside nucleus and how to analyse colocalisation of two protein
I have pics for MCF 7 cells in vitro stained by Phalloidin 488, how I can analyze them please? 😢
Hello sir,I want to use this software to measure the width of the cracks of the reinforced concrete beams, if possible to apply a grid (perpendicular to the crack) on the image this grid to determine the width and thank you in advance please idea me it's urgent.
why do we have to converrt the images from index colour image to gray scale image, i mean is it a general standard for publication??
Sir will you help me for some image processing, related to trichome (hair like structure on leaf) counting ? I am trying but not getting proper results.
Hi,
I am working on phases of polymer blends using Atomic force microscopy.
Please upload a video about how to filter the phase image so that we can easily differentiate phases of the blend..
Super helpful! Thank you
I need to find fibers of a filament in the series of images. each image might have hundreds or thousands of these fibers and I have hundreds of images. What is the systematic approach that can find these fibers in the image?
Thank you! very useful.
Really well explained. Thanks
💚| complete this series plz
Why is the merged image saved in tiff?
Great!
Good to know about this video; you have presented very clearly. Would love to see videos for understanding co-localization analyses at Image J. Thank you !
Hi, you may want to check CoLocalizer apps to quantify colocalization on Mac and iPad: th-cam.com/users/CoLocalizationRS
How can I ask software to segment part of an image? Is software able to detect the edges of a cell image from the background?
Try this article. it is very useful but you need to download the plug - ins required for the segmentation
www.jstage.jst.go.jp/article/plmorphol/29/1/29_15/_article/-char/ja
Copy the title and search for the English version. It's available in Pdf format
Thanks. One more question: I need to find fibers of a filament in the series of images. each image might have hundreds or thousands of these fibers and I have hundreds of images. What is the systematic approach that can find these fibers in the image?
@@zeynabmousavi1736 it depends on what you are trying to investigate in the first place. For instance, in the above article the systematic approach is based on 4 parameters of the microfilaments. According to your test you must decide what the precise effect that you are suspecting to observe. Then you will be able to make analysis for the whole area of your images or for certain fields of your choice. Since you have plenty of them, then you must be comparing something against another. The values you get from the image analysis software can be translated into readable data using a statistical test of your preference.
@@andromeda3780 Lets explain in different way: I have fibers of the cytoskleton scattered inside an image. There are hundreds of those fibers in the image. Assuming that fibers are linear, I want the software to find all those fibers and find a specific characteristics of those fibers (for example find the angle between each fiber a vertical line in the image. So I would extract hundreds of angles from one image and I want to repeat this with several other images that I have. Also I might need to section an image and do the above analysis on a section of the image. Any recommendation in this case would be appreciated.
@@zeynabmousavi1736 in this case try this article journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.1005063
But any way the approach is the same whether you have one image, section of an image or hundreds of them. You must know how to run the analysis using software.