Very nice presentation and straight forward procedure. I will soon have 2 undergraduate students try this kit for research projects on salivary exosomes. We'll definitely get in touch soon.
@@CellGS we ordered 2x EX01-25L Exo-spin™ kits (24 columns), unfortunately the kits too long to arrive due to Covid-19 travel restrictions and cold chain was disrupted. Upon receipt, we stored columns and buffer at 4oC but our fridges broke down over Christmas holidays and we only noticed when we reopened. The students had fun with the kits though, procedure was straight forward but the samples did not elute from the column, although we strictly followed low-speed centrifugation steps. I think the functionality of the columns was affected by inappropriate temperatures during transportation and storage. We'd like to try again with this year's group. Thanks for asking
@@moseskoobotse1875 Sorry to hear you had problems. When you are ready to re-order, please send an email to our info email address and we'll arrange a discount.
Thank you for your video. I would like to ask how much of sample volume would be sufficient for exosome isolation (how many ml of urine/serum)? Thank you.
Not possible! there is no difference between them at all, what they call viruses are actually exosomes! We have been deceived big times The remarkable resemblance between EVs and viruses has caused quite a few problems in the studies focused on the analysis of EVs released during viral infections. Nowadays, it is an almost impossible mission to separate EVs and viruses by means of canonical vesicle isolation methods, such as differential ultracentrifugation, because they are frequently co-pelleted due to their similar dimension [56,57]. To overcome this problem, different studies have proposed the separation of EVs from virus particles by exploiting their different migration velocity in a density gradient or using the presence of specific markers that distinguish viruses from EVs [56,58,59]. However, to date, a reliable method that can actually guarantee a complete separation does not exist. www.ncbi.nlm.nih.gov/pmc/articles/PMC7291340/
Only size based separation cant purify from microvessicles and apoptotic bodies...... Then why you called it exosome? It should be EV for clarification.
@@sharatbarman3352 good point, but I don't think there is a kit on the market that would allow you to separate exosomes from smaller ApBs, MVs or even ectosomes. This is why you ALWAYS need to verify by WB your desired ESCRT biomarkers
Hi Mohammad, Yes it's very effective for both plasma and sera. There are larger sizes for bigger samples and a 96 well version for purifying large numbers of samples.
Chemically do exosomes have anything to do with the immune system other than changes in chemistry per say? I don't know if it would work? Would the use of the trillions per second camera combined with electron microscopes be able to see chemistry in us?
Exosomes transport molecules between all cells, including immune cells. Cytokines are transported in exosomes between immune cells to help orchestrate the immune system. There are many techniques that allow the study of exosomes both in-vitro and in-vivo. They can be visualized with electron microscopy.
Hi :), do you have also something for EV separations at variable sizes? Like 100/200 up to 1000 nm. We need to isolate exosomes as well as microvesicles. Thanks :)
@@CellGS thanks for your reply, but why? Everything bigger than 30nm will be in a void volume anyway so all EVs together if iam not mistaken :). Also when i will the use precipitanion buffer, in the manual it is written that the polymer in the buffer causes that LC-MS downstream method can not be used. Does it mean that it can not be used only immediately after precipitanion or also desalting on a spin column wouldnt help? Thanks :), we need to use it for the LC-MS method.
@@matusjurcik6974 Good question. The reason larger particles >200nm don't come through is because the space between the resin beads isn't big enough. THe 20-200 nm range is because smaller things soak into the cracks in the individual resin beads and larger things just don't get through the gaps between the resin beads efficeintly. So that creates a window of sizes that are purified dependent on the circumference of the resin beads. If you are using LC-MS downstream, we recommend concentrating the sample with other methods such as a micro concentrator. If your starting sample is blood, you won't need to concentrate. You can just add the sample directly to the column. If you have any more questions, please contact tech@cellgs.com to speak with an expert.
@@CellGS Thanks :), ok so it means that there is only Vvoid + Vpore (small contaminants/proteines) and retarded Vvoid alone because some ways can be blocked by bigger (more than200nm) particles right? because bigger particles will stuck ... Also we try to extract EVs from the plasma to screen biomarkers of deseases and we want to use the LC-MS for that.
@@matusjurcik6974 You're rigth. The bigger particles will eventually block the passage of the target exosomes. That's at least in part why there's a limit to the number of times you can reusue the columns. They get progressively slower. If your using plasma, there's no need to concentrate the sample. For screening biomarkers where you're likely to be using large numbers of samples, Exo-Spin 96 is worth looking at.
Dear Sylvia, Exosomes are lipid bilayer particles with a range of diameters from 30 to 150 nm, which are released by most cell types and have an important role in the cell-to-cell communication.
Although not recommended and they are economical enough for single use, it is possible if you flush them out with PBS, if you're not too worried about sample carryover.
Hi John, We only offer solutions for exosome isolation and characterization for R&D purposes. If you are interested in exosomes diagnostics and therapeutics, we recommend that you look into therapeutics companies. Thanks!
This product is available from www.cellgs.com and is for research use only. We sell to research institutes, pharma and biotech companies. There is a lot of research on the regenerative potential of exosomes. As with stem cell therapy, there's also hype and nonsense out there. Just using exosomes purified from any source will not work. I'm not aware of any completed clinical trials showing exosomes work therapeutically. Until properly organized trials have been completed, we can't be sure they work.
Hi Julia I want to use the protocol that you have been demonstrated last time using column chromatography would you please! share me the the type of chemicals(reagent), column chromatography?. And also would you send me the protocol and the required chemicals and instrument for the method I want to isolate in large scale for therapeutic application from the cell culture . Thank you Julia!!!
Hi Stefan, Thank you for your question. This technique may not be how you would go about isolating viruses. Nevertheless, I think it's reasonable to assume that any viruses present in a sample would co-elute with exosomes in an SEC column based on their size. I have no problem with the concept that beneficial viruses exist. But that does not mean pathogenic viruses don't exist. Pathogenic viruses do exist. Vaccines help the body to fight them.
Pathogenic virus organisms exist? Where is the proof of that? Where is the proof that a vaccine stops these highly contagious deadly mutating pathogenic virus organisms from getting inside the body and being pathogenic inside the body? If that was a true statement by you then then those who didn't get a vaccine would be dead of virus organism infection and that's definitely not the case as many people I know have never been vaxxed against any pathogenic virus organism and have no issues living life. Many people have beeng around people who have been told they have highly contagious deadly mutating virus organisms growing by the billions inside their bodies exploding their cells even though they too feel absolutely fine and have been told they are asymptomatic but have lots of virulent highly contagious deadly mutating virus organisms growing inside them but are not sick… Some of these people have been vaxxed and still are told they have these pathogenic virus organisms growing in them so how does that work? Should we all run and hide from these people or accept the fact that the germ theory of disease is a fairytale and so are highly contagious deadly mutating pathogenic virus organisms.
Everything you said was wrong, exosomes and corona are one and the same, exosomes are pieces of a dead cell, the corona, and it can be used as delivery system for the brain, it can carry nano technology, graphene, for brain / machine interface to the quantum computer, with the help of 5g towers.
Thank you. Good video. Simple enough for this old guy to understand :)
Very nice presentation and straight forward procedure. I will soon have 2 undergraduate students try this kit for research projects on salivary exosomes. We'll definitely get in touch soon.
Hi Moses, Just reading back through the comments and wondering how you got on With Exo-spin?
@@CellGS we ordered 2x EX01-25L Exo-spin™ kits (24 columns), unfortunately the kits too long to arrive due to Covid-19 travel restrictions and cold chain was disrupted. Upon receipt, we stored columns and buffer at 4oC but our fridges broke down over Christmas holidays and we only noticed when we reopened. The students had fun with the kits though, procedure was straight forward but the samples did not elute from the column, although we strictly followed low-speed centrifugation steps. I think the functionality of the columns was affected by inappropriate temperatures during transportation and storage. We'd like to try again with this year's group. Thanks for asking
@@moseskoobotse1875 Sorry to hear you had problems. When you are ready to re-order, please send an email to our info email address and we'll arrange a discount.
@@CellGS Will do, thanks a lot.
Can u please say the cost of the kit?
Thank you for your video. I would like to ask how much of sample volume would be sufficient for exosome isolation (how many ml of urine/serum)? Thank you.
Great job Julia - thanks!
very nice demo and I wish I could see your result of exosome density or yield
thank you, amazing video! we need more ladies like this
I think very good presentation. Thank you for your video.
Is the kit suitable for obtaining milk exosome?
How are exosomes differentiated from viruses?
Not possible! there is no difference between them at all, what they call viruses are actually exosomes! We have been deceived big times
The remarkable resemblance between EVs and viruses has caused quite a few problems in the studies focused on the analysis of EVs released during viral infections. Nowadays, it is an almost impossible mission to separate EVs and viruses by means of canonical vesicle isolation methods, such as differential ultracentrifugation, because they are frequently co-pelleted due to their similar dimension [56,57]. To overcome this problem, different studies have proposed the separation of EVs from virus particles by exploiting their different migration velocity in a density gradient or using the presence of specific markers that distinguish viruses from EVs [56,58,59]. However, to date, a reliable method that can actually guarantee a complete separation does not exist.
www.ncbi.nlm.nih.gov/pmc/articles/PMC7291340/
@@yoshicoool6460 what a load of absolute shit.
@@gurpchirp Spoken like a proper moron.
Says 3 replies but none appear. I'm guessing censorship hiding the fact that they are one and the same. Exosomes = so called viruses.
Virus organisms are fakebelieve exosomes are actual things that exist in reality..
Did you do some test to verify the protein contamination in the small vesicles isolation?
Bbmcn s
J l
Is it good for treatment of human diseases ?
Hi can I have more information please how to contact you to order the things for my clinic ?
What is price? What’s made in from
Do exosomes can help in regeneration of teeth in future dentistry
Hi Julia,
How do you isolate microvesicles and apoptotic bodies in the exosome range (30-200nm), please?
Hi Gungun Barman, Exo-spin kits are optimized for isolating exosomes in this size range. Just follow the standard protocol and you'll achieve this.
Only size based separation cant purify from microvessicles and apoptotic bodies......
Then why you called it exosome? It should be EV for clarification.
@@sharatbarman3352 good point, but I don't think there is a kit on the market that would allow you to separate exosomes from smaller ApBs, MVs or even ectosomes. This is why you ALWAYS need to verify by WB your desired ESCRT biomarkers
How much this kit and what's agency
Hi Mohammed, You can see the kits and where to but them here www.cellgs.com/items/exosomes/exospin.html
It is quite effective video regarding the exosomes isolation. Is it suitable for blood serum and plasma?
Hi Mohammad, Yes it's very effective for both plasma and sera. There are larger sizes for bigger samples and a 96 well version for purifying large numbers of samples.
Can anyone buy the kit?
Yes, the kit is available from www.cellgs.com
How to contact you ?
Chemically do exosomes have anything to do with the immune system other than changes in chemistry per say? I don't know if it would work? Would the use of the trillions per second camera combined with electron microscopes be able to see chemistry in us?
Exosomes transport molecules between all cells, including immune cells. Cytokines are transported in exosomes between immune cells to help orchestrate the immune system. There are many techniques that allow the study of exosomes both in-vitro and in-vivo. They can be visualized with electron microscopy.
Hi :), do you have also something for EV separations at variable sizes? Like 100/200 up to 1000 nm. We need to isolate exosomes as well as microvesicles. Thanks :)
Hi Matus, Exo-spin is designed to isolate exosomes in the 20-200 nm size range only.
@@CellGS thanks for your reply, but why? Everything bigger than 30nm will be in a void volume anyway so all EVs together if iam not mistaken :). Also when i will the use precipitanion buffer, in the manual it is written that the polymer in the buffer causes that LC-MS downstream method can not be used. Does it mean that it can not be used only immediately after precipitanion or also desalting on a spin column wouldnt help? Thanks :), we need to use it for the LC-MS method.
@@matusjurcik6974 Good question. The reason larger particles >200nm don't come through is because the space between the resin beads isn't big enough. THe 20-200 nm range is because smaller things soak into the cracks in the individual resin beads and larger things just don't get through the gaps between the resin beads efficeintly. So that creates a window of sizes that are purified dependent on the circumference of the resin beads. If you are using LC-MS downstream, we recommend concentrating the sample with other methods such as a micro concentrator. If your starting sample is blood, you won't need to concentrate. You can just add the sample directly to the column. If you have any more questions, please contact tech@cellgs.com to speak with an expert.
@@CellGS Thanks :), ok so it means that there is only Vvoid + Vpore (small contaminants/proteines) and retarded Vvoid alone because some ways can be blocked by bigger (more than200nm) particles right? because bigger particles will stuck ... Also we try to extract EVs from the plasma to screen biomarkers of deseases and we want to use the LC-MS for that.
@@matusjurcik6974 You're rigth. The bigger particles will eventually block the passage of the target exosomes. That's at least in part why there's a limit to the number of times you can reusue the columns. They get progressively slower. If your using plasma, there's no need to concentrate the sample. For screening biomarkers where you're likely to be using large numbers of samples, Exo-Spin 96 is worth looking at.
Hi, Julia! This kit can be used for exosome isolotion from culture of leishmania?
Hi Gabriela, Yes it will work for exosomes from any source. If you need additional guidance, please contact tech@cellgs.com
What is in exosme ???
Covid 19 lol.
Anything toxic the body is,trying to get rid off.
Louis Alvarez The body’s cells release exosomes when there is disease/illness. It’s not ONLY covid19 that causes exosomes....
Dear Sylvia,
Exosomes are lipid bilayer particles with a range of diameters from 30 to 150 nm, which are released by most cell types and have an important role in the cell-to-cell communication.
Viruses are exosome?
Dr Andrew Kaufman
th-cam.com/video/KGGd7-vvd9Y/w-d-xo.html
th-cam.com/video/fvcEIarencM/w-d-xo.html
Outstanding Mam
Can this column be reuse?
Although not recommended and they are economical enough for single use, it is possible if you flush them out with PBS, if you're not too worried about sample carryover.
@@CellGS thanks
@@CellGS I see you have the Exo-spin™ mini-HD columns, can this one be reuse for 1 more time? Thanks
@@OneStereoSound Yes, you can reuse them. Flush out with plenty of PBS to remove the particles retained in the first use.
At that point, it might be cheaper than the pbs used to clean them lol
How does one reintroduce the exosomes back into the body, intravenously i presume
Hi John,
We only offer solutions for exosome isolation and characterization for R&D purposes. If you are interested in exosomes diagnostics and therapeutics, we recommend that you look into therapeutics companies.
Thanks!
Where can I purchase this cool technology? Does exosomes repair heart and stomach and other organs etc?
This product is available from www.cellgs.com and is for research use only. We sell to research institutes, pharma and biotech companies.
There is a lot of research on the regenerative potential of exosomes. As with stem cell therapy, there's also hype and nonsense out there. Just using exosomes purified from any source will not work. I'm not aware of any completed clinical trials showing exosomes work therapeutically. Until properly organized trials have been completed, we can't be sure they work.
@@michaeljones2781 thanks
@@michaeljones2781 I've found one of the clinics in USA, exosome does work I've tried it , I didn't need to take tablets again
@@icecoolaid2003 really? How much did it cure your illness?
@@lahorepigeon5996 let's talk in mailbox
Is any kind of license needed to use exosomes?
Yes, a pilot's license
it is true exosomes is a nature in our body??
Hi Ahmad, Yes, it is. All cells make and secrete exosomes.
Hi Julia I want to use the protocol that you have been demonstrated last time using column chromatography would you please! share me the the type of chemicals(reagent), column chromatography?. And also would you send me the protocol and the required chemicals and instrument for the method I want to isolate in large scale for therapeutic application from the cell culture . Thank you Julia!!!
Thank you
Very nice
I should've been a scientist ... but instead i became a truck driver 😔
So why can we Isolate these and not viruses? 😂
Hi Stefan, Thank you for your question. This technique may not be how you would go about isolating viruses. Nevertheless, I think it's reasonable to assume that any viruses present in a sample would co-elute with exosomes in an SEC column based on their size. I have no problem with the concept that beneficial viruses exist. But that does not mean pathogenic viruses don't exist. Pathogenic viruses do exist. Vaccines help the body to fight them.
Hi, where is Stefan’s question? Would be nice to see. Thanks.
Pathogenic virus organisms exist? Where is the proof of that?
Where is the proof that a vaccine stops these highly contagious deadly mutating pathogenic virus organisms from getting inside the body and being pathogenic inside the body?
If that was a true statement by you then then those who didn't get a vaccine would be dead of virus organism infection and that's definitely not the case as many people I know have never been vaxxed against any pathogenic virus organism and have no issues living life.
Many people have beeng around people who have been told they have highly contagious deadly mutating virus organisms growing by the billions inside their bodies exploding their cells even though they too feel absolutely fine and have been told they are asymptomatic but have lots of virulent highly contagious deadly mutating virus organisms growing inside them but are not sick…
Some of these people have been vaxxed and still are told they have these pathogenic virus organisms growing in them so how does that work?
Should we all run and hide from these people or accept the fact that the germ theory of disease is a fairytale and so are highly contagious deadly mutating pathogenic virus organisms.
Everything you said was wrong, exosomes and corona are one and the same, exosomes are pieces of a dead cell, the corona, and it can be used as delivery system for the brain, it can carry nano technology, graphene, for brain / machine interface to the quantum computer, with the help of 5g towers.
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NICE EXOLANATION
exosome
:0 wow
thank you
Thank you