How does Spectrophotometer work? Step by step practical. General operating procedure

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  • เผยแพร่เมื่อ 29 ต.ค. 2024

ความคิดเห็น • 8

  • @PriyankaYadavPY
    @PriyankaYadavPY ปีที่แล้ว

    Please share calibration of spectrophotometer by using k2cr2O7 and CuSO4 and analysis of Sulphate and nitrate in water sample

  • @FarooqAhmed-oz5js
    @FarooqAhmed-oz5js 2 ปีที่แล้ว

    Hi. Could u please explain how u can calibrate the Labomed Double beam UVD 32000. FOR G6P-DH .MEANS Where I can load my references sample . And after set wavelength. Which model should select from 1 to 3 in main screen

    • @Laboratoryanalysisofwater
      @Laboratoryanalysisofwater  2 ปีที่แล้ว +1

      Very simply go to photometric measures set parameters, take absorbance and make calibration curve in excel and find out concentration

  • @jayashridutta26
    @jayashridutta26 2 ปีที่แล้ว

    Sir you haven't uploaded the spectrophotometer part of the fluoride determination.. Can you please tell how much absorbance can occur for 1,2,3,4,5 ppm standard.. Are the absorbance fix or vary with the instruments

    • @sufiyaseen2294
      @sufiyaseen2294 2 ปีที่แล้ว

      go to photometric mode . enter one cuvette in referance cell containing ref solution. and another cuvette containg standard 1ppm , take obsorbance meter will prompt you "do y want to enter more samples" than carry on with 2,3,4,5ppm and than sample n than blank.

    • @jayashridutta26
      @jayashridutta26 2 ปีที่แล้ว

      @@sufiyaseen2294 I understand the operation of the instrument, but i want to know whether the absorbance for a particular concentration is specific in preparation of standard curve. Because we can't prepare the curve after trying 5) 6 days. Our curve come on fourth quadrant

    • @Laboratoryanalysisofwater
      @Laboratoryanalysisofwater  2 ปีที่แล้ว

      may vary slightly which depends on instrument accuracy and human error during standard preparation, environment factor etc.

    • @civil7813
      @civil7813 7 หลายเดือนก่อน

      ​Hi sir flouride test can we do in quatitation...uv 1900 spectrometer By keeping 0.5, 1,1.5, 2, 2.5ppm and then sample..it shows correct flouride in sample or no...t.​@@sufiyaseen2294